EMAGE mouse gene expression spatial database

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EMAGE:13200

Sema4f sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain ( MGI:1340055)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017395. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017395_01	euxassay_017395_02	euxassay_017395_03	euxassay_017395_04

euxassay_017395_05	euxassay_017395_06	euxassay_017395_07	euxassay_017395_08	euxassay_017395_09

euxassay_017395_10	euxassay_017395_11	euxassay_017395_12	euxassay_017395_13	euxassay_017395_14

euxassay_017395_15	euxassay_017395_16	euxassay_017395_17	euxassay_017395_18	euxassay_017395_19

euxassay_017395_20	euxassay_017395_21	euxassay_017395_22	euxassay_017395_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13200_wholemount_strong.wlz

13200_wholemount_moderate.wlz

13200_wholemount_weak.wlz

13200_wholemount_possible.wlz

13200_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13200_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9234
Entity Detected:	Sema4f, sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain ( MGI:1340055)
Sequence:	sense strand is shown >T9234 GTCGAGCTCGGCCGAGGGTACCCGAGAGGGGCCGAGGCCCGCGCAGTAGCGGTACTGAGTAGAGGCTGCC CGACGCGCCCCACCCGGCACCAGGCGGAGCCAGAGATGCTGGCCAGGGCCGAGCGGCCCCGCCCGGGCCC CCGGCCGCCTCCGGTCTCTCTCTTCCCGCCGCCGTCGTCGCTGCTGCTGCTGCTGCTGGCGATGCTGAGC GCCCCGGTGTGCGGCCGCGTCCCCCGCTCGGTGCCCAGAACCTCGCTGCCCATCTCCGAGGCTGACTCCT ATCTCACCCGGTTTGCGGCCCCTCATACGTACAATTACTCTGCTCTCCTGGTGGATCCTGCTTCTCACAC ACTTTACGTCGGTGCACGGGACAGCATCTTCGCTTTAACCCTGCCCTTCTCTGGGGAAAAGCCTCGAAGG ATCGACTGGATGGTGCCCGAGACTCACAGACAGAACTGTAGGAAGAAAGGCAAGAAAGAGGACGAATGTC ACAATTTTATCCAGATTCTCGCCATTGCCAATGCCTCTCACCTCCTCACGTGTGGCACCTTCGCTTTTGA TCCGAAGTGCGGGGTTATTGATGTGTCCAGTTTCCAGCAGGTTGAAAGACTTGAGAGTGGCCGGGGGAAA TGTCCTTTTGAGCCAGCTCAACGGTCAGCAGCTGTAATGGCTGGGGGCGTCCTCTACACCGCCACTGTGA AAAACTTCCTGGGGACAGAGCCGATTATCTCCCGAGCTGTGGGTCGAGCTGAGGACTGGATTCGAACAGA GACCTTGTCATCCTGGCTTAATGCCCCAGCCTTTGTCGCAGCTATGGTCCTGAGCCCGGCTGAGTGGGGG GATGAAGATGGAGACGATGAAATCTTCTTTTTCTTCACGGAGACCTCCCGAGTCTTGGACTCATATGAGC GCATCAAGGTCCCACGAGTGGCCCGTGTGTGTGCGGGGGACCTTGGGGGCCGGAAGACCCTTCAGCAGAG ATGGACGACGTTTCTAAAGGCTGACCTGCTGTGTCCAGGGCCCGAGCATGGAAGGGCCTCGGGGGTTCTG CAGGATATGACAGAGCTTCGACCTCAGCCTGGCGCGGGGACCCCCCTCTTTTATGGCATCTTTTCCTCCC AGTGGGAAGGAGCCGCCATTTCTGCTGTGTGTGCCTTCCGACCCCAAGACATCCGGGCAGTGCTGAATGG TCCCTTTAGAGAGCTAAAACATGACTGCAACAGGGGACTACCTGTCATGGACAACGAGGTGCCCCAGCCC AGACCTGGAGAGTGCATCACCAACAACATGAAGTTCCAGCAGTTTGGATCCTCACTCTCCCTGCCAGACC GTGTGCTCACCTTTATCAGAGACCACCCGCTCATGGACAGGCCCGTGTTTCCAGCTGATGGCCGCCCCCT GCTGGTCACTACGGATACAGCCTATCTCAGAGTTGTGGCTCACCGGGTGACCAGCCTCTCAGGGAAAGAA TATGATGTGCTCTACCTGGGGACAGAGGATGGGCACCTCCATCGGGCTGTGCGCATCGGAGCTCAGCTCA GTGTCCTGGAGGATCTGGCCTTGTTCCCGGAAACACAGCCGGTTGAAAGCATGAAATTGTACCATGATTG GCTTCTGGTGGGCTCCCATACTGAGGTGACACAAGTGAACACCAGCAACTGTGGCCGTCTCCAGAGCTGC TCAGAATGTATCCTGGCCCAGGATCCCGTGTGTGCCTGGAGCTTCCGGCTCGATGCTTGTGTGGCCCATG CAGGCGAGCACCGTGGGATGGTTCAAGACATAGAGTCAGCAGATGTCTCTTCTTTGTGTCCAAAAGAACC TGGAGAACATCCGGTAGTGTTTGAAGTTCCAGTGGCTACGGTGGGCCACGTGGTCCTGCCGTGCTCCCCC AGTTCTGCCTGGGCATCCTGTGTGTGGCACCAGCCCAGTGGAGTGACTTCGCTCACCCCACGAAGGGATG GGCTAGAGGTGGTGGTGACCCCAGGGGCCATGGGGGCTTATGCTTGCGAGTGTCAGGAGGGTGGAGCCGC CCGTGTGGTGGCTGCTTATAGCTTGGTGTGGGGCAGCCAGAGGGGGCCCGCAAACCGGGCCCACACCGTT GTGGGGGCTGGGCTGGTTGGCTTTTTCCTGGGTGTTCTAGCAGCATCTCTCACTCTCCTCCTGATTGGTC GCCGTCAGCAGCGGCGGCGACAGAGGGAGCTTCTAGCTAGAGACAAGGTGGGCTTAGATCTGGGGGCTCC ACCTTCTGGGACCACAAGCTATAGCCAAGACCCTCCCTCTCCTTCACCTGAAGATGAACGGCTGCCCCTG GCCCTGGGTAAGCGGGGCAGTGGTTTTGGTGGCTTCCCTCCACCCTTCCTGCTGGATTCTTGCCCGAGCC CAGCCCACATCCGGCTCACTGGGGCTCCTCTGGCCACGTGCGATGAGACGTCTATCTAAAGCCAGGAAAA TGACTGCCAGCCATGAGCAATCTCTGGAACTAGCGGCTACGAAGACCAGGGTCACTGAACCTGGGAGACG ATCATGGCTGCTCCATTCTCTTGGAGTCTGTGTGATCACACGTCAGTGTCTGTCCTCTGGACCTGGATGA GCTGAGGGCCAGACCTTTGCCTGATTCCTGATTCTCATGAGAGATCAACCCTGTAGCCCTCTGCTATGGC CTCTTGTCTTGGGCCCATCATCAGCTTGTGGGGTGGAGCAAGGACACAGGCTCTGATTTGCTTATGGTGG GCCCCCGGAAAGAGGGAGCACTGCGGGGTGGTTGGAGCGCGTGTGCCCTGCATCCGTGTGGTGCCTGTAT GATGATTTTCCAGTTTGCTGACTCTGGGGAGCGCATGATCCCCGGACTGCCTTGAGATCTCTCCCAACTC AGTTTCCCTTTGTCCCTTGAAGAGTGTGTGTCTATACACTGGTGTGCCTCGAAGACCTGTCCAGGTGTGT GTGGATGACAGGGCCGGTGCCTAGGTGCTTTTGGGGGGTCGGAGAGGAAGGTTGGAACGGGGGACAACTT TACCCTCGGTAGCCAGTGAGGGAAACCACACGACCCCATCACCCCACAGCGCTTCTTCAACTTTGAGCAA AGTTCCCAAAGTGACCTTCTGGTGGGAAGGGCAGCAGGACACGTGGCCCCGTCCTTCTCCTCATCTTTCT CCTCTGGCTGCCACCACTGCCGTGCCACCGCTGCTTTTTCCCTGGCTGGAGTGGAGGCTGAGCCCTCTTT CCTTGGCTTCCATTTAAAATGAACTTCACAACATTCTAGCTATTGAGGGATGACAAAAGGAACCCAGAAA AGTGTGTAGGAAATTACAAGCAGGTTCAAGAAGATTTGCCTCAGTGACTTTCACCCTTGCCCTAAAGCAG GAGTCCCCTAGCAGGACTCCCTGAAATTGTATGCAAAGAAATGTCTGTCCATGTGTGGCATGTGTGCGTG CGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATGGCTTTCATCAGATTCTCAAGG CCTTAATGAGGTTAAAGGACCATGGTCCATAGTCACCACACTTGGGCCACATAAATGGAGGAGGTGTTGC TGTGAGGCAGTTCCTCCCTGGCCTGCTGCCTGAGGCCAGTCCCTGGACACACTGCTGCTGGAGATCCCAC CTCTCTCCAGTCCTCCTCGGAAGCTAGTGAAGCTAGGCTCTGCACGTGCTTGAAGACACCATCGCCTCCT TTCTTGCTTCGTTATTCCTGTGTGCGCTGCATCTGCTCCGTCCTGCTCTTGGCCTGTGAATGTGCCTCGT CCATCCCTGGTGAGGGACCTCGAACCCCGGTGAGGATGCAGCCCTTTCCAGTTGAAGTTTCTGTTCTACT TTCCTTGACAGCAGCCTGTGAATTACTCAAGAGTCCCCTTGGGTTTGGAGTTACCAGTGGCTTTGAGTAG GATCTTTGGCACGTAGTCTAACCTAGGGACATTGGTTATTCATCTGTAAAGTGGGGATATACCTACCTCA GGGTTGCTGCAAGGATCAAACGAGGAAACGTATGAACAATAAAGCATTACCCAGAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6834138 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834138 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13201 same embryo
	EMAGE:13202 same embryo
	EMAGE:13199 same embryo
	EurExpress:euxassay_017395 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source