EMAGE mouse gene expression spatial database

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EMAGE:13201

Helb helicase (DNA) B ( MGI:2152895)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017320_01	euxassay_017320_02	euxassay_017320_03	euxassay_017320_04	euxassay_017320_05

euxassay_017320_06	euxassay_017320_07	euxassay_017320_08	euxassay_017320_09	euxassay_017320_10

euxassay_017320_11	euxassay_017320_12	euxassay_017320_13	euxassay_017320_14	euxassay_017320_15

euxassay_017320_16	euxassay_017320_17	euxassay_017320_18	euxassay_017320_19	euxassay_017320_20

euxassay_017320_21	euxassay_017320_22	euxassay_017320_23	euxassay_017320_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13201_wholemount_strong.wlz

13201_wholemount_moderate.wlz

13201_wholemount_weak.wlz

13201_wholemount_possible.wlz

13201_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13201_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9304
Entity Detected:	Helb, helicase (DNA) B ( MGI:2152895)
Sequence:	sense strand is shown >T9304 GTTGTTTTGAGCCAAGTTCGCCGAAGAGATAATGGCTAGGCAGGATCGTCTGCGCGAGCTCCTGGGCCCT CTGCACCCGTACAAGAGCGACGACGAGGAGGAGGATTGTGCGCAGGAGGAGGAGGGGGAGCAGGAGGAGG AGTTTGTGGATGCCGAGGAACTCTGCAGCGGGGGCATCAAGGCGGGCAGCCTCCCTGGGCGCGCGCGAGT GTCTATCCCTGATGAGTATACTAAAGAGAAATGTACCGTGTACGGACGTTTCCCACTCAAAGGTCCGTGG TGGCGCGTGAAGGTCCAGGTCCTGAAACCCCAGCGATCCAGGAGCTATCAAGTTCAAGGCTTTCCGGCAT ACTTTCTACAGGTTGATATGTCGCCACCAGATCAAAAACAAATCTGTTCGCTTTTTCTTAAAGAATGCAA CTTAGCCTCTGAGCGCATACAGGAATTTTTAAAATGGGTAGAAAAAGTGTCAAGCTTTGAAAATCTACAC TTTGAAAATCTTTGGGAAACATTAAGGCTTTTCTACAGAGAAACCGAAAAGAAAGATAAAAAGCTGTCTA CACCAAGGGAGCAGCAGGGTGAGGAGATGAGAGTGGAAAAATCATTCGCATTTATAAGTGCAATGGTAGC TCTGCAATTCCCCAAAGTCATGGAATTCCTTCCATCGCTTTTCCCTCGACACTTCAAAAGGCTCATCAGC TCGAGTTCTGATTGGGTGCTGGGATGCATAGAAGATGTTCTGGGTACACAGCCGTGGAAGCTGGGCTTCA GGAGGATAACCTACAGAGAAATGAAACTAGTGCGCTGTGAGGCCAGCTGGACAGCGTTCAGTCAGTGCCC CTCGCTGCTCCAGCTGATGACGCCCCTGCAGAAGAATGCATTGGTCATATATTCTAAGCTGAGGCAGACA TGCAGAGAAGACGGACACACGTACATTGAAGTAAAAGACTTGACCTCGGGCTTGTCGGAGCATATGTCAT TCGAGGAAGCGTGTCAGTCTCTGGCGTTTCTGAAGGACATTGACGTGGTCATTTATGAGAAGGACTATGT CTTTCTTTCTGAACTGTACGAGGCCGAGCAAGACATCGCCTCCTCCATCTGCGAGCTGATGAGCAGACCT CCGTGGCACTTAAAAGTGGACGTGAAGAACGTCCTGGCGTCAATTAGAGGCGCAAAGCCCAACGATCCAG GAAGTGCTGAGGCAGTGGAAGGGAGCAAGCCGGAGGAGGTGGGGTCAGAGCAGGGTGACAGTGTTTTAGA CGCCCAAGATGGCGATGACCATGTTAGAAGCAATGGCGAACACGTCGCAAATGCAGAAATAAACGACGTC CCACTGGATCAAGATCAGGTGGTCGCCCTGGAGACGATCTGCGCCAACGCGGTGACTGTCCTCAGTGGGA AAGGCGGCTGTGGGAAGACCACCATTGTTAGCCGTCTTTTTAAGCACATGGAACATCTGGAAGAAACGGA AGTGCAGCAAGCCTGCGAGGATTTTGAACAAGACCAGGAAGCCTCAGAAGAGTGGCTTGACTGTCCTAAG CAGAGCCCAGCCGGTGTGGACAAGGCTGTAGAAGTCTTGCTCACCGCACCTACAGGGAAGGCTGCTGGCT TATTGAGGCAGAGGACCGATCTTCCGGCTTACACGCTGTGCCAGGTCAATTACAGCTTCTATATGTGGAA AACGAAAAATGAGGTGGACAAGCCGTGGAAATTCTCCACGGTTCGAGTTCTGGTTGTGGACGAAGGGAGT TTGGTGTCTGTAGGGATCTTCAAGTCAGTGTTACAGTTGCTGTGCAAACACTCCAAGCTGTCCAAGCTCA TCATCCTTGGTGATGTTAGACAGTTGCCCAGTATTGAACCTGGTAACATGCTGCAGGATGTCTTTGAGAC TCTTAAGTCAAGACAGTGTGCCATCGAACTAAAGACGAACCACAGGACAGAGTCTCAGCTGATTGTGGAC AATGCTACAAGAATCTCCAGACGTCAGTTTCCAAAATTTGATGCTGAACTAAATATCTGTGGTAATCCTA CATTACCTCTCTCCATTCAAGATAAGACATTTATCTTTGTGAGACTCCCTGAAGAAGACAGCAGATCTCA GTCGTCTAAAGGTGAACACCGATCTAATTTATATACTGCAGTTAAAACTTTGCTTCAAGGAAAGGACTTC TGCTCTTTTGAAAGTTCAAAAACATCGCAATTTATTGCATTTAGAAGGCAAGACTGTGACCTCATCAATG ACTGCTGCTGCAAGCATTACACAGGCCACCTCATCAAAGACCATGAGAAGAAACTCATCTTTGCCGTCGG TGATAAGATCTGCTGTACCCGGAATGCATACCTCTCAGATTTACTCCCCGACAAAGACCAGGAAGCCGAG GGGAAAGGCTATGGAGACGCCCCTGACGACGATGCTAAAATCAAACAGGATTTCGAAAGCAGCATCCGCC TGTGCAACGGGGAAATATTTTTCATAACACGGGATGTAACTGACGTAACTTTCAAAAGAAAAAGACTTCT GACCATTAATAACGAGGCCGGTTTGGAAGTGACTGTGGACTTTAGCAAACTAATGGCAAACTGTCAGATC AAGCATGCCTGGGCAAGGACCATTCATACTTTTCAGGGGTCCGAGGAGAACACAGTCGTCTACGTCGTGG GGAAGGCAGGCCGCCAGCACTGGCAACATGTTTACACGGCAGTGACCCGGGGCCGCTCCAGAGTGTACAT CATCGCGCAGGAGTCTGAACTCAGGAGTGCCACCCGGAAGCGCGGTTTCCCCAGGCAGACGCGTTTGAAA CACTTCTTGCAGAAGAAGCTCTCTGGCAGCTGCGCACCCTCAACAGGTTTCGCATCCCAGCCTTCGAGCC CTCGAGTCGGAGGAAGACCTGACACCCAGCCTCCAGCGTCACACCTCTGTAGAACCCCAGACAACAAAGC CACTGCAGACAGCGCCAGAGGCGACGAAAGATGGCTGTCAGCTTCTGTTAACGATGACGTGGATACGGAT GAGGAGTCAGCACAGCTCAGAGGGTCCAAAAGAATCGGTGACGGCTTCCCGTTTGATGAAGAAAGCCCCA GTAAATTCCGTATGGTTGAAGCGCCATCTCCACAGGTGTCCTCTGTGTTTCAGAATATGAGGTTGAATAC TCTCACTCCCAGACAGCTTTTTAAACCCACCGACAATCAAGACACAGGTACGGCAGGTGTGGCGGACGAC GCCAACGATCCCAGCAATCAGGAGATGGAAATGTGAGGATCTGCGTTCATGGCCAGCCTGGGCAACATCG TAAGACCCTGCTCAGCCCATACCACCACCACCACCACCACCACTGAAAACCTCAAAGCAGAAAAAAAAAA GTTTACTTCATATTAATCAAGAAATTATTATTTTTTTCCTCCCAAGACAAGATGAACATTTTAGCTTCAA AGTTTCAAACTTCAAACTCTGAGAAGACAGCATTATGGTTTTGGGCATTTGTAATTTTGGGCACATGACC CAAAATAAAATTTTTATATTATTTTTAAATCTAAGTTTCAAAAGGAGGTATTCTAAAATCACCCAATCCA AGAAAACCATGATGATTTCAGCCTCTGTCCTCGTGAGAACAGTTGACAGGGGACACAGTTAGGGGAAAAG GTGCTGTATGCTTAAAACACTCCCGTTTTAGGGGCTGCTGGATGTTTTGTGATAATGCCATGTGATCATT GCGGATCTCCTTTGCCAGCACACTGAACCAGCAAAGGTGGACTGATTGTACCTGTTGATTGTAACACATT CAAGGATGCTGTTTGTGGCGACATCCTTGCTGTGATGCTGTGGCCATCACTCCATTCCTGTGCCTGGGGC TCTGCGTACGTGGGGCTTGGATATGCCTTTCTGAGTTGGGAGTAAGTTTAGCATATATCCCATTGTAGGG AAGACTTTCTCTTAAAGCATATTGGTTTTTAAATATTTATTACCTAGTGGTACTGTGGCTTGATCGTGTC TTCCCAAACCTGTGTGAGTTTTTTTTTGGGGGGGTGGGGGTGGGGGGTGGTTTCGAGACAGGGTTTCTCT GTACAGCCCTGGGGCTGACCTGGAACTCACTTTGTAGACCAGGCTGGCCTCAAACTCAGAAATCCGCCTG CCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCGCCACCACTGCCTGGCATGGCAGAGTGGTAGAGAG AGTTTTTCCATCTTTCTCCCTTCTTTTGGTGCTGGAACTGAAGCCAAGGTCTCATTCCTTCTAAGAAAGC ACTTTACTGCTTAATTTTATTCCTGATCCTAAACATAACTTTTATATGTACCAGGAAGTAAACAAACAAG ACCAGTCATGTGATGCAGCTGATGGTAATGCTTTTGTTATTTCGGGTGCCCTGGGACTGATCTGCAGCAT TTCTGAGATAGACCTTTGTGAGAAATAAATCCACTATTCTTTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6843107 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6843107 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13202 same embryo
	EMAGE:13199 same embryo
	EMAGE:13200 same embryo
	EurExpress:euxassay_017320 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source