EMAGE mouse gene expression spatial database

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EMAGE:13202

Setx senataxin ( MGI:2443480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017357. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017357_01	euxassay_017357_02	euxassay_017357_03	euxassay_017357_04

euxassay_017357_05	euxassay_017357_06	euxassay_017357_07	euxassay_017357_08	euxassay_017357_09

euxassay_017357_10	euxassay_017357_11	euxassay_017357_12	euxassay_017357_13	euxassay_017357_14

euxassay_017357_15	euxassay_017357_16	euxassay_017357_17	euxassay_017357_18	euxassay_017357_19

euxassay_017357_20	euxassay_017357_21	euxassay_017357_22	euxassay_017357_23	euxassay_017357_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13202_wholemount_strong.wlz

13202_wholemount_moderate.wlz

13202_wholemount_weak.wlz

13202_wholemount_possible.wlz

13202_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13202_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9246
Entity Detected:	Setx, senataxin ( MGI:2443480)
Sequence:	sense strand is shown >T9246 CGCACTTCAGTTATGCGCAGTGGGAAAGCTGAGTGTTCATTATGCATACAGACACAAGATACCTTGCCAG CCAGTGTAAAAAATCTTACAAGATGCATTGTGATCAGTTCTCTGGTAACTACACAGAGGAAATTGAAAGC CATGTCTCTGTTGAGTAGTCGGAACCAGTTGGCCAGAGCTGTTCTAAATCCAAACCCCATGGACTTCTGT ACCAAAGATCTACTAACTACAACTTCTGAAAGAATTGTTGCCTACTTAAAAGACTTTAATGAAGACCAAA AGAAAGCCATAGAAACTGCATATGCCATGGTGAAACACTCACCATCCGTTGCCAAAATCTGTCTGATTCA TGGACCACCTGGAACAGGAAAATCCAAAACTATTGTTGGTCTCTTGTACCGCTTACTGACAGAGAACCAG AGGAAGGGCCATTCAGATGAAAATTTCAATGCCAAAATCAAACAAAATCGAGTCCTCGTGTGTGCACCTT CCAATGCGGCGGTTGATGAACTTATGAAAAAAATTATCCTCGAATTTAAAGAAAAATGTAAAGACAAGAA GAATCCTTTGGGAAACTGTGGAGATATAAATTTAGTACGACTAGGTCCAGAAAAGTCTATTAATACAGAG GTCTTAAAATTCAGTTTGGACAGCCAAGTCAACCATAGAATGAAAAAAGACTTGCCTTCTCATATTCAAG AAATGCTTAGAAGAAAAGAAATTCTAGATGCTCAACTTGATGAGCTTTCTCGTCAGCGAGCTCTGTGCCG AGGTGGGCGGGAGATGCAGAGGCAAGAATTAGATGAACACATTGCCATAGTCTCAAAAGAAAGGCAAGAA CTTGCTTCGAAAATTAAAGAGGTTCAGGGACGCCCACAGAGAGCACAGAATACCATCATCTTGGAGTCCC ATGTCATCTGCTGCACATTGAGCACTAGTGGTGGTTTATTGCTTGAGTCTGCTTTTCGAGGGCAAGGGGG TGTCCCCTTCAGTTGTGTCATCGTAGATGAGGCTGGGCAGTCTTGTGAAGTTGAGACACTTTCTCCACTC ATCCACCGCTGCAATAAACTTATCCTAGTAGGAGACCCCAAACAGCTCCCTCCCACAGTCATCTCTATGA AAGCACAGGAGTATGGCTATGACCAGTCGATGATGGCCCGATTCTGCAAGCTGCTGGAAGAGAATGTGGA GCAGAACATGATTGGCAGGCTGCCGGTGTTACAGCTTACCATTCAGTACAGGATGCATCCAGACATATGT CTTTTCCCTTCTAATTACGTTTATAACAAAAACCTGAAGACAAATAGATTGACTGAATCCATTCGATGTT CATCAGAGTGGCCATTTCAGCCCTACCTCGTGTTTGATGTTGGAGATGGTTCAGAACGACGGGATAATGA CTCATATATAAATGTTCAAGAAATAAAGTTGGTGATGGAAATAATTAAACTTATTAAAGAGAAAAGAAAA GATATTTCCTTCCGAAATATTGGCATAATAACCCATTACAAGGCTCAGAAGACGATGATCCAGAAGGATT TGGAGAAAGAATTTGATAAGAAAGGACCTGCAGAAGTGGACACAGTAGATGCCTTCCAGGGCCGGCAGAA AGACTGTATCATCGTTACCTGTGTCAGGGCAAGTGCTGTGCAAGGCTCAATAGGATTCCTGGCAAGTTTG CAGAGATTGAATGTCACCATTACACGAGCCAAGTATAGCCTCTTTATCCTTGGACATTTGAGGACTCTGA TGGAAAACCAGCATTGGTATGAACTGATTCAGGATGCTCAGAAGCGTGGGGCCATTATTAAGACCAGTGA CCCAAACTATAGACATGACGCAATGAAGATTCTGAAACTGAAGCCTGTGCTACAGAGGAGTCTGACCCAT CCCCCAGCCACTGCCCCGGAGGCACCCAGACCCCAGGGTGGCTTGCCCAGCAACAGACTGGACAGTGGAC TTGCCACAACATCTTTTGCTGCTTCTTTGTATCACACACCCTCTGATACTGTCACTTCAAAGGGACCTGA AAGGCCACTTCTTCAAGACCGACTTCGAGATCCACGACTACTCAGGAGATTGGATGCTGAAGCCAAGGGA ACATTCCTGAAGGATCCACAGCCCGTTAGCCCCCAGCTCCCCGGGGTAGTTCATCTCTTGGGAGAGCCTG GCTTCCCTGTGGTTTTCCAGGACCTAGGTTTTGTTGTGCCGCCATCCACTGCCATTGTTGCTCCTCTGGG CAGCCACAGGTCACCTATGCAGGCTGAGCCACCACCTGCTCACCCTGCTGCTGCCGCCTCCACAAGTAAG AGGAAGTACAGTGACCCAGATGCTGGGCTCAGCCACAAAAGAGAACCCAGGGCCTTCAGTGGAGAGCAGG GGAGGCATGGCTCTGTGACTCATCACGTGCTGAGAAGCACTGACTGGGACAGGAGGAGGCTGGATGACAG CAGTGCCAAGAGGAGACAATTTCTATAGTAGCACCTCGTGAGGAGACTGTTGTCCACAGGCCAGCTAGTG GTACCCTACTGAGAAGTTGGGTTTTGGTTTTGTTTCCCTTGAAGGGTCGCTGTTAGAGGATGGAAGTAAC TTCTAATTCTTGATCTGTTTGTTGGTCTTGTTTTCAGTACTTTTTGCCAGTTGTATACACTTGGAGAGGG AATTTGTATGCCTGTAATCTTGTTCTTGAGGTCAGAAATTCAAAACATTGGGAGCTTTTGTTGTAAAGGT TAAACTGTGAATCCATATAGCAAATGCAGATCCTTTTACAGTGTAAACCACATTTCCTGCCTCAGCCTAA AGCACTGGTCATTTTCCAACAGAACAAACTTCCAATTTAAAGAGAGAGAGGAGGACAGATCCTTTCAACC ATTGTGTGGTAGATGAATTTAACAGCCTCAGTACTGGGAGTTAGTCAAGTTTGAACAAGTAGCTAGTGTC TTCAGCTGGGGTTTCGTGGGCCTTTAGAAAGCAGCACGTTGGGAGTTGTGCTCATAAGTGCACTTTGAAA CAAGGCAGCTTGGCACAGGGCATGACTGTGACAAGTTCATTTACCTATATTGGTGTATAGGTTTTCTGGC TAAAAATCTTTCCCTTCTCAAAACGGAAGACCAATTCTTGAATGGTCATTCATGCTTGCAAAGTTTTGTG AGGTTGATGTAACTGGGGAATTGTAGACAGCTTAGCAAATGGAGGATTTGTGCTGGCTTGGTTGGTCTGG CCTGGAAGAGTCCATTTAGGATGCTTGTGAAGGCTTGAGAGTTGCCCGGAAAATTAAAAAGGATGCATTT TAAGGGATCCCATGGGAGCTACACAGGGGCAGACCAGATAGCATATCTCAGGCAGGAGAGAAACTCCATG AGAAAGAAGAGCAGCCGTCTGGGCTGCAGGACCATCCAGTAGGTAGGTGAAAGTGCAGTCTATGTTGGGT CACTGCAGGCACTGGACTCCATCAGACCTGGTGTGGTTGTGAGAGGAAAGCAGAGGGGCCTGGAAGTCAA GTCGGGACAAGGGAGGGAGGGCTGTTTAACCAGTCTCTATGTAGAAGGGCATGTGAGTGTGAGGTCTCTG GTAACTACTGTGTGCACAGCCCGTTAGCTGCCTTGGGGCAGGAAGACAGCCCTAAAACTTAGTGTTTGCT GTTAGGAGGGATTTTTGCCATCTGTTCTTACACAAGGGTTTTGTGTTAGAAATTCTGCTGAAGGAGGTTT TGTTTGTTAAGAGCTTTGAAAAAATGCATTTTGCCAAGTTTCAACACTTGAGTTTCCTGATCTCTTTCTG CTAGTGCCCAGACATGAACCCTAATATCTGCTGAATACTTGTAAAGCTGGGGAGTAGGCACCTTCTCAGA GCCCTTCAGCCTCCTGGATGTCCCCAGCTAAGTGGGAAAGAAATCCTGCTCTGGCTAGGGCAGGGGTTCA GTAAGCTCCTGCATATGACATTGGCAGGAAACCTCACTGTCACTTGAAATTTTCTTTCCTAGTATCTGTG AGAAGTGCTCAGTTTAGCCAAAAGTGTGCTTCTGAGAGGGGAATAACAACAGCAATAGCCAGGACGTTTC CTTCTCTAAAGACCCAAGAAGTACATTTGTAAAAAGAAGATTTTCCAAAACATGTAGTTGGCTGCTCTCC AATGAGTATGTTGTAGGACATTGTGTGTTCATCTGCTGGCTCATGGCTAGCCACATCCGAGTATTGGAAT TTGTATTGCAGCCTGCTTATTTTGTGAATGTAAGGGCTGCTGGGATTAGACTCTGGCCAAGGGATCTTCC TTCTCAGCAAGCAGACTTGCCTTAGAACTGTCTCACAGCAGAATGTAGTTGCATTATATTAGATCTGAAA CCTTATTTATTCCAGTTGGTAAAGGTATTTGCAGAAAGTGTTTGCTGTGTCAAGGGGCCTTTCCTGACAG TGCTGTCAGAAATGGTCAACCCTGACAAGATGAAAAGGTCACTTTGTCCTTGCTGTGGTTAAAAGAACAT GCCCTGACTACTTTCAAATGTTATTTCAAGGTGAATATATTATACCTGTCTTACTTTGCTTCTCTCTTGA TTTACCACAGTTTGTCCAGTTATGTTTTCATGAATTATCAGGAGTAAATTTGAACAAAAACATGTGAATA ATCTCCTGTAAATGTGTTTTATAACAAGAGTGTGCTAAACTATTTTCTAAAAATTACTTGTGCATTTTGG GAATCTTTCTGTAGCTTTGTGTTTTGTATGCTCTGGATTCATAACTGGATTTTGGCAAATAGTAAACCTA TTATCTTTGTTAAAACCTAAAATTTAAGAGAAAATGGCAGAAATAAAAACAAACAAGAAAACTGAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834956 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834956 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13201 same embryo
	EMAGE:13199 same embryo
	EMAGE:13200 same embryo
	EurExpress:euxassay_017357 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source