Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13303

Epha2 Eph receptor A2 ( MGI:95278)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303
"Pseudo-wholemount" of euxassay_017705. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017705_01 euxassay_017705_02 euxassay_017705_03 euxassay_017705_04
EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303
euxassay_017705_05 euxassay_017705_06 euxassay_017705_07 euxassay_017705_08 euxassay_017705_09
EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303
euxassay_017705_10 euxassay_017705_11 euxassay_017705_12 euxassay_017705_13 euxassay_017705_14
EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303
euxassay_017705_15 euxassay_017705_16 euxassay_017705_17 euxassay_017705_18 euxassay_017705_19
EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303 EMAGE:13303
euxassay_017705_20 euxassay_017705_21 euxassay_017705_22 euxassay_017705_23 euxassay_017705_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13303Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13303_wholemount_strong.wlz
13303_wholemount_moderate.wlz
13303_wholemount_weak.wlz
13303_wholemount_possible.wlz
13303_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13303_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31652
Entity Detected:Epha2, Eph receptor A2 ( MGI:95278)
Sequence:sense strand is shown

>T31652
AATGGATGTTCCTCGCCACAGGTCCGCAAAGGCCCCTCTATGTCCGGCTTTCCTCTCTGCCTCCCAGTCT
TGTCTCTGACCCTCCCTCTCTTCCTCTTCATCCACAGAGACATCAAAAGGATCGGAGTGCGTCTTCCTGG
CCACCAGAAGCGCATTGCCTACAGCCTGCTGGGACTCAAGGACCAGGTCAACACAGTGGGGATTCCTATC
TGAGTCCATTGGGGCTGTGCCCAACAATACTTGAAGAGCCACAGTGGTCTCCCTGCCGACCTGGTGCTGG
CCCACTGGAACTTTATTTATTTCTGTTTCCTCGTCTATGCCTCCCTGAGGACTCTGCAGGGGGCTTTTGA
ATGACACCCTGGCCTGAGCCTGGGAAACTTGGATGCTGGTCAGGGCTCTCTTTCCCCTGCAAAGGACCCA
GCTAAGCACTTAGCAGTTTGCCATGGCCTTCCCAGCATCCCCTGAGGCTAAAGTTCCACCAAGACCGTCG
A
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2811918), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61564. Forward Primer - name:061564_F_IRAV08-11_J09_Epha2, sequence:AATGGATGTTCCTCGCCA; Reverse Primer - name:061564_R_SP6_IRAV08-11_J09_Epha2, sequence:ATCGACGGTCTTGGTGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13306 same embryo
 EMAGE:13304 same embryo
 EMAGE:13305 same embryo
 EurExpress:euxassay_017705 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS