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EMAGE:13304

Tmem88 transmembrane protein 88 ( MGI:1914270)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304
"Pseudo-wholemount" of euxassay_017633. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017633_01 euxassay_017633_02 euxassay_017633_03 euxassay_017633_04
EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304
euxassay_017633_05 euxassay_017633_06 euxassay_017633_07 euxassay_017633_08 euxassay_017633_09
EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304
euxassay_017633_10 euxassay_017633_11 euxassay_017633_12 euxassay_017633_13 euxassay_017633_14
EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304
euxassay_017633_15 euxassay_017633_16 euxassay_017633_17 euxassay_017633_18 euxassay_017633_19
EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304 EMAGE:13304
euxassay_017633_20 euxassay_017633_21 euxassay_017633_22 euxassay_017633_23 euxassay_017633_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13304Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13304_wholemount_strong.wlz
13304_wholemount_moderate.wlz
13304_wholemount_weak.wlz
13304_wholemount_possible.wlz
13304_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13304_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40166
Entity Detected:Tmem88, transmembrane protein 88 ( MGI:1914270)
Sequence:sense strand is shown

>T40166
CTGGGTCTGAGGTGCTTAGAGTCAAGAATCCTGTCGATTGCCCTCCTGGTCTCTCAAGGACTTGAAACAA
GCCCAGGGGTGTACTGCCCAAGCCGGTTCCTTACCGCGGCCTGAGCCGGATCCTACCATCCCTTTGATGG
AACAGCTGAATGTGGACGTAGAGCTGGCGAAAATTCCCATGTCCCAGAAACGTCATTCACATTTGATTCC
TGAACGTTTGGGCTGGACCCTGCACATCTTCTTTCCCTTCCCTATTGTAAATAAGAGAGCTGGACCTTGT
GCCTTCCTTTGCTGGTGCAGCTCCTCCAAAATTTACAGGACTGAGGGACAGGGCTCACACAAATAAGAGA
GAAGCCATCACAAATAATAAAAAAAAATTATAAAATGAATGCACTCTGTGAATACAGGGGTGGCACATAT
CCAATCGCTCCTGCACTGGGCTCAGGAGTAGGAAACGAATCCTCAAGACTACAAATGGGCAAATCAGAGA
GCATCTCTCAGCATCCTGCCTTTTCCTTATCGCATTAGTCCATTCTTCCTGATCAGACCCCAGAGTTGTC
TGGCCCTGACCCCAAAGTCACTTCCCCATTAATAGCGCAGAGAGGGCCTTGTTTCGGCTGCCTACCCAGC
TCCCCTTGGGGACCCGAGCCCGTTCTCTGACCCCAGCTTAAAGGAACTGAGTGTTGCCAGGCCGGGGTGG
GAAACAATGGATCTTCCGGGGCAGGATGACACCGCTTCCCGCTAGAAACTGAAGCAGGAGCGTGGGAGGG
AAGATGGAGGGGCCCACCCTAGGGCCTGCTGCCTTAAGCTCCTAACTTGAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94899. Forward Primer - name:094899_F_cDNA_2600017H02Rik, sequence:CTGGGTCTGAGGTGCTTAGAGT; Reverse Primer - name:094899_N_SP6_cDNA_2600017H02Rik, sequence:CCTCAAGTTAGGAGCTTAAGGCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13306 same embryo
 EMAGE:13305 same embryo
 EMAGE:13303 same embryo
 EurExpress:euxassay_017633 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS