EMAGE mouse gene expression spatial database

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EMAGE:13327

Grm7 glutamate receptor, metabotropic 7 ( MGI:1351344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017413. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017413_01	euxassay_017413_02	euxassay_017413_03	euxassay_017413_04

euxassay_017413_05	euxassay_017413_06	euxassay_017413_07	euxassay_017413_08	euxassay_017413_09

euxassay_017413_10	euxassay_017413_11	euxassay_017413_12	euxassay_017413_13	euxassay_017413_14

euxassay_017413_15	euxassay_017413_16	euxassay_017413_17	euxassay_017413_18	euxassay_017413_19

euxassay_017413_20	euxassay_017413_21	euxassay_017413_22	euxassay_017413_23	euxassay_017413_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13327_wholemount_strong.wlz

13327_wholemount_moderate.wlz

13327_wholemount_weak.wlz

13327_wholemount_possible.wlz

13327_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13327_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9372
Entity Detected:	Grm7, glutamate receptor, metabotropic 7 ( MGI:1351344)
Sequence:	sense strand is shown >T9372 AGAGCGCGAGCGGCAAGCCAGGGAGCGGGGAGCGCAAGCTGGTGCACGGGCCAGCGGAGCTACAGTGCCA GGTGCCAAAGACTGGCTGACGCCCTGGGACCCCCCACCCCTCTCCTGCTGCACCTCCTCTGTCTCCTCCA CCCTCTGTGCCTACAGAAGTCCCCGGGGCTTCCCCCGAAAGGAGCTCACCTCGAGAAGCCGGGACCTGGG AGAGCCCACCAGCATCCCCTCCAGCGCCGCCGCCGCTACCGCAGCAGCATCCGGAGCGGCATGGTCCAGC TGGGGAAGCTGCTCCGCGTCCTGACTTTGATGAAGTTCCCCTGCTGCGTGCTGGAGGTGCTCCTGTGCGT GCTGGCGGCGGCGGCGCGCGGCCAGGAGATGTACGCCCCGCACTCGATCCGGATCGAGGGGGACGTCACC CTTGGGGGGCTGTTCCCAGTGCACGCCAAGGGTCCCAGCGGAGTGCCCTGCGGCGACATCAAGAGGGAGA ATGGGATCCACAGGCTGGAAGCTATGCTTTATGCTCTCGACCAGATCAACAGCGATCCCAACCTGCTGCC CAACGTAACGTTAGGCGCGCGGATCCTGGACACTTGTTCCAGGGACACTTATGCGCTCGAACAGTCGCTC ACTTTCGTCCAGGCGCTTATCCAGAAGGACACCTCCGACGTGCGTTGCACCAACGGAGAGCCCCCGGTTT TCGTCAAGCCAGAGAAAGTAGTTGGAGTGATTGGGGCTTCGGGGAGCTCCGTCTCCATCATGGTAGCCAA CATCTTGAGGCTTTTCCAGATACCTCAGATTAGTTATGCATCCACGGCTCCTGAACTCAGTGATGACCGT CGCTATGACTTCTTCTCTCGCGTGGTCCCACCTGATTCCTTCCAAGCCCAAGCTATGGTAGACATTGTAA AGGCATTGGGCTGGAATTATGTATCTACTCTTGCATCTGAAGGAAGCTATGGAGAGAAAGGTGTGGAGTC CTTCACACAAATTTCCAAAGAAGCAGGTGGACTCTGCATTGCCCAGTCCGTGAGAATTCCCCAAGAACGC AAAGACAGGACCATTGACTTTGATAGAATTATCAAACAGCTCTTGGACACCCCCAACTCCAGGGCTGTCG TGATTTTTGCCAACGATGAGGATATAAAGCAGATCCTTGCAGCAGCCAAAAGAGCTGACCAAGTAGGACA TTTTCTCTGGGTTGGGTCAGACAGCTGGGGTTCCAAAATCAACCCACTGCATCAGCATGAAGATATTGCA GAAGGAGCCATCACCATCCAACCTAAGAGAGCGACTGTGGAAGGATTTGATGCTTACTTCACATCCCGGA CACTTGAAAACAACAGGAGAAATGTATGGTTTGCCGAATACTGGGAAGAAAACTTCAACTGCAAGTTGAC AATTAGTGGATCCAAAAAAGAAGACACGGATCGCAAATGCACAGGACAGGAGCGAATTGGAAAAGACTCT AATTATGAGCAGGAGGGTAAAGTACAGTTTGTGATCGATGCTGTCTATGCCATGGCACATGCCCTTCATC ACATGAATAAGGATCTGTGTGCTGACTACCGGGGAGTGTGCCCAGAAATGGAGCAAGCAGGGGGCAAGAA GTTGTTGAAGTATATCCGCAATGTTAACTTCAATGGTAGTGCTGGAACCCCAGTGATGTTTAACAAAAAT GGTGATGCTCCAGGACGATATGACATCTTCCAGTACCAGACAACAAACACTACCAATCCTGGTTATCGTC TCATTGGGCAGTGGACAGATGAACTGCAGCTCAATATAGAGGACATGCAGTGGGGCAAAGGAGTCCGGGA GATCCCACCCTCTGTGTGCACATTGCCATGCAAGCCTGGGCAGAGGAAGAAGACACAGAAGGGAACCCCT TGCTGCTGGACTTGTGAGCCCTGTGATGGATACCAGTATCAATTTGATGAGATGACATGTCAGCATTGTC CCTATGACCAGAGGCCCAATGAGAACCGAACTGGCTGTCAGAACATCCCAATCATCAAACTGGAGTGGCA CTCCCCCTGGGCTGTAATTCCTGTCTTCCTGGCAATGTTGGGGATCATTGCCACCATCTTTGTCATGGCA ACCTTCATCCGCTACAATGATACACCCATCGTCAGGGCATCTGGGCGGGAACTCAGCTATGTTTTATTGA CAGGCATCTTTCTCTGCTATATCATCACCTTCCTAATGATTGCCAAGCCAGATGTGGCAGTGTGTTCCTT CCGACGTGTCTTCTTGGGGTTGGGTATGTGTATTAGTTATGCAGCCCTTTTAACAAAAACCAATCGGATT TATCGCATATTCGAGCAGGGCAAGAAATCGGTGACAGCTCCCAGACTCATAAGCCCAACATCACAACTGG CAATTACCTCCAGTTTAATATCGGTGCAACTTCTAGGTGTCTTTATTTGGTTTGGGGTTGACCCACCCAA CATCATCATAGACTACGATGAGCATAAGACAATGAACCCAGAACAAGCCAGGGGAGTTCTCAAATGTGAC ATCACAGACCTTCAGATCATTTGTTCTTTGGGATATAGCATTCTTCTCATGGTCACATGTACTGTGTATG CCATCAAGACTCGGGGTGTACCAGAGAATTTTAATGAAGCCAAGCCCATTGGATTCACTATGTACACGAC TTGTATCGTATGGCTTGCCTTCATCCCAATATTTTTTGGCACAGCGCAATCAGCAGAAAAGCTCTACATA CAAACCACCACGCTTACAATCTCCATGAACCTAAGTGCATCAGTGGCGCTGGGGATGCTATACATGCCGA AAGTGTACATCATCATTTTCCACCCTGAACTCAATGTCCAGAAACGGAAGCGAAGCTTCAAGGCCGTAGT CACAGCAGCCACCATGTCATCGAGGCTGTCACACAAACCCAGTGACAGGCCCAATGGTGAGGCAAAGACA GAACTTTGTGAAAATGTAGACCCAAACAGCCCTGCTGCAAAAAAGAAGTATGTCAGTTATAATAACCTGG TTATCTAACCTGTTCCATCCCATGGAACCACAGAGGAGGAAGACCTTGAATTATTCTGTCACCCAGCCTG GCGTAGGACTCTTTTGGTCCTGCCTGCTTCCTATCTCTGGAGGAGCTTCCCCACGCGGGAGAGCAATGGC AGAGGATCCACACATCCTGAACAGCTGCTTTATGAGACACCTTTACTTTATCTTGGCTTAAGAAGTCATT GGCATCAGCACTGCCATCTTGGCTGCAATTCTGGACTCCCTACAGCAAAGGGAGAGTTGAGATTCAAGTC CCACCCGGCTCTTTTGGATGAACCGCTGAGAGCCTCAGGACTGTTTTTGGGGGGCTGACTTGTCTTTCTA CGTACGTACTTCCAGGCTGCAAGGTTTTGAAATTTTCTGTACAGTTTGTGAGGACATTTGCACTTTGCCA CTCAATGTCGTACCTCGGTTCACTGTTTGTTTTTGAATGCCCTGTTTTCATAGAATCCTATCCTCTCAGA TGGTGGAATCTTTGGGGAAACTTTAAAGTAACTAATTTTTATTTAAAAAAAAAATCTCGGCAGACATAAG AGAACACAACCCCCCATGCACATCTGTAAATGACTGTATGGTTATGATTATAGTACCACGAAAAATCATG TTATTTTTTTTTAAGACACACACAAAAAGATACTTAAAGACCAAAAACTGTGCTGGGAAGATATGCCCCA CCTATCTTTGGTACATGGTAGGTGTTGTGATGGAAATGGAAAGCATTGGTTAAATGGAATAGAGGTCTTG ATCTTTGGAATGCATGCCGGTAATGTATTTTACAGTACATGTTAATAATTTATGTTTAATATTTGTATTT GTGTTCTCTTTTGTTATTTTACTTAGGGTATATGGATATTTTGCAATAATTTTAATGATTCTTAAGCTAT TTGAAGGAAAGAATATGGATTTCTCATGTCTTGAGGTTTTGTTCATGCCCCTATGACTGACCAATGTGAT AAGGACTTTAAAAAAGAAGCATGTATGTTTTTTACTGTTTGTAAGAAGTACTTTCCTTAATCTTGCTGCT TATGTGTCAATTTAGTGGAAAAGAAATCCCTTGCTGAAAAAAAAAATCCCTTTCCATTCTCTTCCAGTTC TGTGGTATTGTCCAAGAATGTATCAATAAAATACTTTGGTTAACTTTTTTAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853901 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13329 same embryo
	EMAGE:13326 same embryo
	EMAGE:13328 same embryo
	EurExpress:euxassay_017413 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source