EMAGE mouse gene expression spatial database

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EMAGE:13352

Zfp161 zinc finger protein 161 ( MGI:1195345)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017545. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017545_01	euxassay_017545_02	euxassay_017545_03	euxassay_017545_04

euxassay_017545_05	euxassay_017545_06	euxassay_017545_07	euxassay_017545_08	euxassay_017545_09

euxassay_017545_10	euxassay_017545_11	euxassay_017545_12	euxassay_017545_13	euxassay_017545_14

euxassay_017545_15	euxassay_017545_16	euxassay_017545_17	euxassay_017545_18	euxassay_017545_19

euxassay_017545_20	euxassay_017545_21	euxassay_017545_22	euxassay_017545_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13352_wholemount_strong.wlz

13352_wholemount_moderate.wlz

13352_wholemount_weak.wlz

13352_wholemount_possible.wlz

13352_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13352_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8429
Entity Detected:	Zfp161, zinc finger protein 161 ( MGI:1195345)
Sequence:	sense strand is shown >T8429 GTGGCCCGTCGCTGGCGGGCACAGCTCAGGGGAGCTTTTGTTTAGGAGAGAATGCTGAGGGCTCGCTGCA TCCGGCGGCTCATTGGCTGCGAAATGCGAATGTGGGCTCCCCACGCGCGGACTGCGCCCCATGTCTTTCG CGGGGCCCCGGATTCCCCACTCCCAAGTTGTGCGGTCGCAACAGGGAAGTGGTGTGGCCGCCATTGTCGC AGCTGCTCCGGAATAAGGAGGCGGCTGCGGCTGCTTATATCTGGCTTGCCCCTGATCAGCCTGAGTCTGA TCTGAAGATTTTGGTGTTTAAGGCATTAAGAACATAGCCTGAATTGTTCATGGAGTTTTTCATCAGTATG TCTGAAACCATAAAATATAATGATGATGATCATAAAACGCTGTTTCTGAAAACACTGAACGAACAGCGCC TGGAGGGAGAATTCTGCGACATCGCCATCGTGGTTGAAGATGTAAAGTTCCGAGCCCACCGGTGTGTCCT CGCCGCCTGCAGCACGTACTTTAAGAAGCTTTTCAAGAAGCTAGAAGTAGACAGCTCGTCCGTCATAGAG ATAGATTTCCTCCGCTCTGACATATTTGAAGAGGTGCTGAACTACATGTACACCGCCAAGATTTCCGTGA AAAAGGAGGACGTCAACTTGATGATGTCGTCCGGGCAGATTCTCGGTATCCGGTTTTTGGATAAACTGTG TTCTCAGAAGCGCGATGTGTCTAGTCCGGATGAAAGTAACGGCCAGTCGAAGAGTAAGTATTGCCTCAAA CTAAACCGCCCCATCGGAGACGCTGCTGATGCTCAGGACGATGATGTGGAAGAAATCGGGGACCAAGATG ACAGCCCTTCTGATGACACGGTAGAGGGCACTCCCCCGAGTCAAGAGGACGGCAAGTCTCCCACAACCAC TCTCAGGGTTCAGGAAGCAATTTTGAAAGAGCTGGGGAGTGAGGAAGTTCGGAAAGTGAACTGTTACGGC CAGGAAGTGGAGTCCATGGAGACTCCCGAATCCAAAGATCTGGGCTCTCAGACCCCTCAGGCCTTAACCT TTAATGATGGGATGAGCGAAGTAAAAGATGAACAGACTCCGGGCTGGACCACGGCGGCCAGCGACATGAA GTTCGAGTACTTGCTCTACGGTCACCATCGAGAGCAGATCGCCTGCCAGGCGTGTGGGAAGACGTTCTCC GATGAAGGCCGGCTGAGGAAACATGAGAAGCTCCACACCGCTGACAGGCCGTTTGTCTGTGAGATGTGCA CAAAAGGTTTCACCACCCAGGCCCACCTGAAAGAACACCTAAAAATCCATACAGGGTACAAACCCTACAG CTGCGAGGTGTGCGGAAAGTCATTCATCCGCGCCCCAGACCTGAAGAAGCACGAGAGGGTTCACAGCAAC GAAAGACCATTTGCGTGTCACATGTGTGACAAAGCCTTCAAACACAAGTCTCACCTCAAGGACCACGAGA GAAGACACAGAGGGGAAAAGCCTTTTGTGTGTGGCTCCTGCACCAAGGCCTTCGCCAAGGCCTCAGACCT GAAGAGGCATGAGAACAATATGCACAGTGAGAGGAAACAGGTCACCCCCAGTGCCATCCAGAGTGAGACA GAACAGTTGCAGGCCGCAGCCATGGCCGCTGAGGCTGAGCAGCAGCTGGAGACAATTGCCTGTAGCTAGA GGTGATGGGTTAGGAACACTGGGCCTGGGAGGTGGATGCTAAGATTGCATTGGCTGTAGTCAGTGAACAC CTTTGACCGCTTTGTGGCAATTTACAGCGTTGTGCTGGCTGGACCTAAGACAGTGCGCACTCCGGTTAGG TGATTCTCAAATTTCTCAAGGCAGTTATGTTCCTACGTTCTGACCAAACAGTTTGCTTTTTTTATCTTTT GGTTTGTGTGTGCGTGTGCGTGTGCGTGTGTGTGTTGTCTAGTATTCATATTCCCAGTAAGCTCCCCTAC CCTTCTTTATGGTTTTAATTTGAAATCTCCTTTGTCTGGTTTAAAATGAGTGGCTTCTGTTTCATTCTTA ATTCCTCAACTCTTGCCAAGCTGGTGAGTGCACTGCACAGAGTCATGGAATAAATTGATTTCCTAGTCCC AGTATATGTGACTTATGTCAAAACAGCAGTTTTAATAACTGGATACAAGACCTTTATATGGATGCAGAAA ATGTTGGCACGCGCTGACCCAGAACACTGCAGAAGTACAAAACCAAATTTGGAAAAATGGAATGGTTTTA GTTTTATATTTGGTTTGTGATTTTTAAATCTCTCTCTCTATATATATATATCATTGTTTCTCACTGTTCC TTGGACAAATAATGGTTGCTTTGCTGAAGTGTTCCTTCATCCATTACAATGGCCCCTGTACCCACCCCAA ATGCTGCAGGTCATGTATACCTCAGGATGAGTGAGACCACCAGGATGCTGGTGGTGTTGGGACTCAGTTT CCCTTTCATTCAGTGTGGCCATGCTGTCTGTGTGTGTCCGTGCAATGGACCAGCATCGACAGGAGAAGAG TCTGACACAAGGTTTTCACAGATACAAAAAACCCCTAGGTCTTGAAATCGTGACTGATTTCAGAAATGGT TTTGTATTTAAGATCAAAATTAGGACAAGCAGCAGCAATGTTTCTTCAAATGCGTTTTTGTAGTTTACAG ATGGTCAGGCCTCGGGGTCTCTGCATCTCTGCAGCACTCAATTTCCCTTGGAGCAGATGCTTCCTTAAAT ACGTCTGAAACCCTTCACGTGTGTAAATAGTATTGTTGGTCTTATGTATTTCGAGAAAAGGGACAGCTGC ATTTTTTTTCTTTCCTTTTTATTTTTTTTATACAGTGGATTAAAGTACCTTTCATTGGCAACACATATCA GACCCCTTTAACCAATATTAAAGACTATCAGACCAAATGTTTATGTTTTCCAAATATGTCTTATCACTGG TTGCAGTTTTCAATAGAAGCTGATTGCCCTGTCATAGCCATAAATCGGAATTATGAACTTTGTTTCAGAT TGGGTGTACTAAAACGCTCCTTTGACTATTTCCTTAAGGATATATTGAAATGCCCACAGAGTTTGAATTA TGTTCTGTAGGAAAGTGTAGACAGTTGTTTTAGGATGTGTACAGTTGTAGAAGGTGCCAACTTTAAAAGC TCATGTTTGGTTCTTAAGGTTTTCTTTTTAAAAAAAAAATGCATTAAAGTAATTTCGTTACATGGCCTGC TGTACTAACATCTGTTATTACTGTCGTGAGAAAAATTTTATTTTTAAAACCGGTGTCCATGTTGTGCCAG AGATTGTAGGATGGAGGGGAGGCTACCTTTAAATTTTTTTCTTTATTTATCGTTAAAATGTTTCAGCTTA AATTAATCTTTCCTGAGTGTTTTTTTTTCTGTTTTGAAGGCAATCCCATCAGTTCGTCTGTGCAGTTAGT AGGAGGGGCCGGACTGGTGATCCTAGGCTGTGTTAACCAAAGCAAATGTGAAATCAGCTGGCATCCTGTT GATAACTACTTCAACAAACCATATTTTGGGCCTTTGTTTTTGTATATAAAGATAATGATACACTCAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5697838 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5697838 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13349 same embryo
	EMAGE:13354 same embryo
	EMAGE:13351 same embryo
	EMAGE:13353 same embryo
	EMAGE:13350 same embryo
	EurExpress:euxassay_017545 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source