EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13353

Pde1a phosphodiesterase 1A, calmodulin-dependent ( MGI:1201792)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017536. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017536_01	euxassay_017536_02	euxassay_017536_03	euxassay_017536_04

euxassay_017536_05	euxassay_017536_06	euxassay_017536_07	euxassay_017536_08	euxassay_017536_09

euxassay_017536_10	euxassay_017536_11	euxassay_017536_12	euxassay_017536_13	euxassay_017536_14

euxassay_017536_15	euxassay_017536_16	euxassay_017536_17	euxassay_017536_18	euxassay_017536_19

euxassay_017536_20	euxassay_017536_21	euxassay_017536_22	euxassay_017536_23	euxassay_017536_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13353_wholemount_strong.wlz

13353_wholemount_moderate.wlz

13353_wholemount_weak.wlz

13353_wholemount_possible.wlz

13353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9301
Entity Detected:	Pde1a, phosphodiesterase 1A, calmodulin-dependent ( MGI:1201792)
Sequence:	sense strand is shown >T9301 GCTAAGAGATGCTACTGTGTAACGCTTGAGATTTGTAACGGACTGAGGAATGCTTGTCCTAGCCGCTGAA ACTGACTGCACCTGTGTTGTCCTAGGAACATCATCATGGGGTCTACTGATACGGACATCGAAGAATTGGA AAACGCCACTTACAAGTATCTCATTGGAGAACAGACCGAGAAGATGTGGCAGCGCCTGAAGGGAATACTA AGATGCTTAGTGAAGCAGCTGGAGAAAGGTGATGTCAACGTTGTTGACCTAAAGAAGAATATCGAATATG CAGCATCAGTACTGGAAGCTGTTTACATCGATGAGACAAGAAGACTGCTGGACACAGAGGATGAACTCAG CGACATCCAGACTGACTCCGTCCCATCGGAAGTCCGGGATTGGTTGGCTTCAACTTTCACACGCAAAATG GGGATGATGAAAAAGAAGCCTGAAGAAAAACCAAAGTTTCGCAGCATTGTCCATGCTGTCCAGGCTGGGA TTTTTGTGGAAAGGATGTACAGGAAGAACTATCACATGGTTGGTTTGACATATCCTGCAGCTGTCATAGT GACACTCAAGGAAGTTGATAAATGGTCTTTTGATGTATTTGCCTTAAATGAAGCAAGCGGGGAGCATAGC CTGAAGTTTATGATTTATGAACTCTTTACCAGATATGATCTTATCAACCGCTTCAAGATTCCTGTTTCTT GCCTAATTGCCTTTGCAGAAGCCTTAGAAGTTGGTTACAGCAAGCACAAAAATCCATATCATAATTTGGT CCATGCAGCTGACGTCACTCAAACTGTGCATTATATAATGCTTCATACAGGTATCATGCACTGGCTCACT GAACTGGAAATTTTAGCCATGGTCTTTGCAGCTGCCATTCATGATTATGAGCACACAGGAACAACAAACA ACTTCCATATTCAGACGAGGTCGGACGTTGCTATTCTGTACAACGACCGCTCAGTGCTTGAGAATCACCA CGTCAGCGCAGCCTACAGACTTATGCAAGAGGAGGAAATGAATATTTTGGTAAATTTGTCTAAAGATGAC TGGAGGGATCTTCGGAACTTAGTGATTGAAATGGTCTTAGCGACAGACATGTCAGGGCATTTTCAGCAAA TTAAAAACATAAGAAACAGCTTGCAGCAGCCGGAAGGGATTGACAGAGCCAAAACTATGTCCCTGATACT CCATGCAGCTGACATCAGCCACCCAGCCAAAACTTGGAAGTTGCACTACAGATGGACCATGGCCCTAATG GAGGAGTTTTTCCTGCAGGGAGACAAAGAAGCTGAATTAGGGCTTCCATTTTCTCCACTTTGTGATCGGA AGTCAACAATGGTTGCCCAGTCGCAAATAGGTTTCATTGATTTCATAGTGGAGCCAACGTTTTCTCTCCT GACGGACTCAACAGAGAAAATTGTCATTCCTCTTATAGAGGAAGCTTCAAAGTCACAGTCTTCAAACTAC GGGGCAAGCAGCTCGTCCACCATGATTGGGTTCCATGTTGCTGACTCTCTGAGGCGGTCAAATACAAAAG GCTCCGTGTGTGATGGGAGCTATGCCCCAGACTACTCGCTCTCAGCTGTGGATCTGAAGAGTTTCAAAAA CAACCTGGTGGACATCATTCAGCAGAACAAAGAGAGGTGGAAAGAGTTGGCTGCCCAAGGTGAACTTGAT CTTCATAAGAACTCAGAAGAGTTAGGAAATACTGAAGAAAAGCATGCTGACACACGTCCATAGGTGTGAA CAAACTTTAAGAGTTCAGTTGTTTTAAACACAACAGGAAAATGAAAGCAGCATGAAAAACACACAAAGAC CTTGATTTTCTAAAAAAATAAAATAAAATGTTTGGCTCAGTCCATTTTAGTGGACTCCTCTGTAAGGAAG AAAAGCATAAATTTCAGTCTAGTAACTGAGCCACAAAGTTCCCACGAGTTATCATTAGGATCCTGGCCAC TGTTTTTGCCATGGTTTTCCTTCCATGATTTGGTCATCTTAGCCAGAGAAATCTTTTAAGGCTGAAAGCT GGATGCTTTCAAGTTTTCATCATTTTTAACTTGTATAGAAGATGGAATGGATTTCAATATACAACTTGCA CTGTGTTACTCCAGAGTGACGCAACAACACAATTGTCAACAAGCAAGGACAACAAGAAGAAAAACAAACA AACAAACAAAAAAAGAGTAAAAGAAAGGCAAAACAAAATGATGAAAACCCTGTTTTCTTGCAATGCTGAT CTGGTAAGACTGTCTTGTTTTAAATGCATGGGAACAGAATGAGAATCAGATCTGTCTTCAGAAGCGCCAG CAACAGCTGACCGTAAGACCTGCCGCACAGTGATTGGATGAACGCCTCTATGGGCCTCCAGCTGGGAAAC TACAGTGCCATCTTCTCCTGAAGTCATCGACCATCCTGCAGCCATCCACTGAGTTTTAAGGCACTAGTGT ATCACCCATGTGTGTGTGGTTATATTATAGGTCGCAGTTTGCTGTAGGGCCAATGTAACAAGGAACATCA GCCATTGCAGGAGAAGGGCACTCAAAGAGAAGACAGAATTTTCCTTCTGGGATGTTGTTACCACTAGAAT ATAAAGATATATTACAGGCTGAGGCATAAGATGTGTGTGTGTGTGTGTGTGTTTGTGTCTGTGTGTCTGT GTGTCTGTGTGTGTGTATGTTGTAGATGTGTGCACTCGTGCTCAGAGGTGTATATGGAAACACATACTTT TCTGTACAGCTAAAACTGTTATGTGATGTGACTTTTTAATTTTACTGAAGAGAAATGAACTGCAACCCTC AGAGGCCCTGCTTCTTGGTCATGTTTTGCCTGAGCATGTGTGAAGTAGCTAACAGGAATGGTCAAGCCCA ATTAGATGTTGTAGTATTTTCACTGTACATACAGTTCAGGGATTGCGAACTTCGTGAAAGGTGACAATTA GATGGAATGAACAGTTACTGTCAATTAGGTGATGTGCAGAAAGGTTCACCCATCCTAATGAGTTGCTTCT CGCACCTGTGTTTCCCCCCAGGTTCCTCAAGGCTAAGCAATATTTCAGAGAGGGACATGACAAGAAGCTT TTAAGCCTAAGTTGTGAGGTCTTTATAACTCACAATTTAGATCTACTTTAATGTGTAAGCCATATTCTCT ATGCATATTTAGTATGCATTTATTGTATTTAAGATATATTGTGAGTAGACTGTATAAAATGCTTTTAAAT GTTCTATGAATTAGATTACTAGTGTTATTATTGTCTAGATCTTGTTAAATAATTGAATTGTATTATAGCC ATTATAATGTGATGGATATTTTAAATATCAACAGTAAAGATTGATCTATAATAATGTGTTGAGATTAATA GAAATGTGTATGTTAGTAGCCTTTGTCCTTTAAAATGGACATTTTATTTCTATTTATGGAGAAACTGTAA CTACTGCTTTTCAAATCTTCTTTTTAAAAATGAGATATTTACTATCTAGAAAACATTCTGCTTTCAAAGT AAAATCTGAGAGTCTAAGAAGTCTAATTACTATTGTTCCTAATTGAGCCAGTTGTCTTTCAGTAAAGCTA GAATCTAAGGAGAGAAGAATAAAATGAAGCTCTGCTCCTCTCATGGCGTAGACAATACATCTATTTTGTT CCTTTGATCTCTATGCTTGGTTAATGTAAATTAAAGAATATGCACATTGATCAGTGTATGTCTGCTAGCC TAATTGTCCAGGCTATGTGACTTACAGAGCACTGTGACCAACCGACTTGCAGGGGTAGCTGGGGAGGCAC ACTGCTTCAGAGCCATCACACTGCCTGGACCATGCACGCTACTCCTCACCTCCCTCCCTTTTCTTCCTGT CTCCACGATGCCCAATGTGTCTGATATTTTGCCTTTGGCCTTCCACACTCCTTCCTTCCTCTCTCCACAG GACAGCTCCTACTGGAAGATAATAAGTAGGGGTCACACTGGAGATCACATGAGCCTCCAAATATACAGAT AATGGTGTGAAAGGCCACACCATGAGGCGGGACTATCTCTCTCCAAAGGCTGCTGCACATGCTTTGCATT TGTACTCACAGTGACCCACCATATACTTGCGAGTTCATCTGCCTTTTCATTTAGCCAGAACTTTACTTTT TAACATGACAACCTATGATTCTTGTGTTTTTATTATGCTGCGACTCTCTGTATTTCCTACTATATATGAT TGCTGAATAAATCATATTCGATGGAAATTATCCTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6842675 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6842675 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13349 same embryo
	EMAGE:13354 same embryo
	EMAGE:13351 same embryo
	EMAGE:13350 same embryo
	EMAGE:13352 same embryo
	EurExpress:euxassay_017536 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source