EMAGE mouse gene expression spatial database

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EMAGE:13379

Pak3 p21 protein (Cdc42/Rac)-activated kinase 3 ( MGI:1339656)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017534. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017534_01	euxassay_017534_02	euxassay_017534_03	euxassay_017534_04

euxassay_017534_05	euxassay_017534_06	euxassay_017534_07	euxassay_017534_08	euxassay_017534_09

euxassay_017534_10	euxassay_017534_11	euxassay_017534_12	euxassay_017534_13	euxassay_017534_14

euxassay_017534_15	euxassay_017534_16	euxassay_017534_17	euxassay_017534_18	euxassay_017534_19

euxassay_017534_20	euxassay_017534_21	euxassay_017534_22	euxassay_017534_23	euxassay_017534_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13379_wholemount_strong.wlz

13379_wholemount_moderate.wlz

13379_wholemount_weak.wlz

13379_wholemount_possible.wlz

13379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8071
Entity Detected:	Pak3, p21 protein (Cdc42/Rac)-activated kinase 3 ( MGI:1339656)
Sequence:	sense strand is shown >T8071 AAGAGGTAATCCCCAGCCCATCTGAGTTCACTTTGCATCTCAATTTTGTTCTTCAACCTCTTTGATCCTC TGCCAGCTTTGAGTCATCTTCAGACGTGGAGCTGTGAAATCAGCTGCAACTGAAAATGTCTGACAGCTTG GATAACGAAGAAAAACCCCCAGCTCCCCCACTGAGGATGAACAGTAACAACCGAGACTCTTCAGCACTCA ACCACAGCTCCAAACCACTGCCCATGGCCCCGGAAGAGAAGAATAAGAAAGCCAGGCTTCGCTCTATCTT CCCAGGAGGAGGGGATAAAACCAATAAGAAGAAAGAGAAAGAGCGCCCAGAGATCTCTCTTCCTTCAGAC TTTGAGCATACGATTCATGTGGGTTTTGATGCAGTCACCGGGGAATTCACTGGAATTCCTGAGCAATGGG CACGACTACTCCAAACCTCCAACATAACAAAGCTGGAACAGAAGAAGAACCCACAAGCTGTTCTTGATGT TCTCAAGTTCTACGACTCCAAAGAAACGGTCAACAACCAGAAATATATGAGCTTTACGTCAGGAGATAAA AGTGCCCATGGATACATAGCAGCACATCAGTCGAATACCAAAACAGCTTCGGAACCTCCTTTGGCTCCTC CTGTATCTGAAGAAGAGGATGAAGAAGAGGAAGAGGAAGAAGATGACAATGAACCTCCGCCTGTCATTGC ACCAAGACCAGAGCATACAAAATCAATCTATACTCGCTCCGTGGTTGAGTCAATTGCTTCACCAGCTGCA CCAAATAAAGAAGATATCCCACCTTCTGCTGAGAATGCCAATTCCACCACTTTGTACAGGAATACAGATC GGCAAAGAAAAAAGTCCAAGATGACGGATGAGGAGATCCTAGAGAAGCTGAGAAGCATTGTGAGTGTTGG GGACCCAAAGAAGAAATATACGAGATTTGAAAAAATTGGCCAAGGGGCATCAGGAACTGTTTACACAGCA CTAGACATTGCGACTGGACAAGAGGTGGCCATAAAGCAAATGAACCTTCAACAGCAGCCCAAAAAGGAAT TAATTATTAATGAAATTCTTGTCATGAGGGAAAATAAGAACCCCAATATTGTCAATTATTTAGATAGCTA CTTAGTGGGTGATGAACTGTGGGTAGTCATGGAATACTTGGCTGGTGGCTCTTTGACTGATGTGGTCACA GAAACCTGTATGGATGAAGGACAGATAGCAGCTGTCTGTAGAGAGTGCCTCCAAGCTTTGGATTTCTTGC ACTCAAACCAAGTGATCCACAGAGACATAAAGAGCGACAACATTCTCCTCGGGATGGATGGTTCTGTTAA ACTGACTGATTTTGGGTTCTGTGCTCAAATCACTCCTGAGCAAAGTAAACGAAGCACTATGGTGGGAACT CCCTATTGGATGGCACCTGAAGTGGTAACTCGAAAAGCTTATGGTCCAAAAGTTGATATCTGGTCTCTGG GAATCATGGCCATTGAAATGGTGGAAGGTGAACCCCCTTACCTTAATGAAAATCCACTCAGGGCCTTATA TCTGATAGCCACTAATGGGACCCCAGAGCTCCAGAATCCTGAGAGACTGTCAGCTGTATTCCGTGACTTC TTAAATCGCTGTCTTGAGATGGATGTGGATAGAAGAGGATCTGCTAAGGAGCTTTTGCAGCATCCGTTTT TAAAATTAGCCAAGCCTCTGTCTAGTCTCACTCCTCTGATTATCGCCGCAAAGGAAGCAATTAAGAACAG TAGCCGTTAGAACTGCAAGCCTTACCCCTCACGGTCTCCAGGATGAGTAAGACTGAAATAAAACTCTGCT GCAGGATCCACAGAAGAAGAGACAGTCAAATGGAGTGGGGGCTCTTTAACTTTTAAGTGAATAGAAACTT CTTATAAACCTTTTTCCTACTCCCTCAGATTATGTAATTTATTTGTAAGCCTGAACTGCAGCCCACACAG GGCAGCAATGTCGAAGTAGCCATTAAGTGGCCACTTCCACCGTGAAGCGAAAGAGCCAGTAGTGAATCCC CTCATTTTGTGCATTTACTTTGAAGAAAAAAAGATTTCTCAAAGATGCACACTCCCTCTTCATAGTGCTG TGTTTGTTTTTAAGTTAGAGAGTAGTCCCTCTTTCATTCGAACCTCTTTCAAAATCCCTTACCCAACGTG ATGTTTTTTCACTTGCATTGTCATTAGATGTTCAGAAAAATAAAAGATGTCAAAATGTTTTTTTAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30060082 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30060082 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13381 same embryo
	EMAGE:13382 same embryo
	EMAGE:13377 same embryo
	EMAGE:13380 same embryo
	EMAGE:13378 same embryo
	EurExpress:euxassay_017534 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source