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EMAGE:13390

Cd24a CD24a antigen ( MGI:88323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390
"Pseudo-wholemount" of euxassay_017776. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017776_01 euxassay_017776_02 euxassay_017776_03 euxassay_017776_04
EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390
euxassay_017776_05 euxassay_017776_06 euxassay_017776_07 euxassay_017776_08 euxassay_017776_09
EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390
euxassay_017776_10 euxassay_017776_11 euxassay_017776_12 euxassay_017776_13 euxassay_017776_14
EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390
euxassay_017776_15 euxassay_017776_16 euxassay_017776_17 euxassay_017776_18 euxassay_017776_19
EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390 EMAGE:13390
euxassay_017776_20 euxassay_017776_21 euxassay_017776_22 euxassay_017776_23 euxassay_017776_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13390Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13390_wholemount_strong.wlz
13390_wholemount_moderate.wlz
13390_wholemount_weak.wlz
13390_wholemount_possible.wlz
13390_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13390_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T540
Entity Detected:Cd24a, CD24a antigen ( MGI:88323)
Sequence:sense strand is shown

>T540
CCTCGAGNCTGTTGGCCTACTGGGTTGTTTCGTTACTACTAATTTAATACCGTAAGCTCTAGGTAAAGTT
CCATGTTGTTGAACTCTGACTGTTCTCTTTGGAATTGAACGTTTTGCATCCTCCTCCTGTGGCTTTAGGT
CTGACATTGTATTTGACCTTTACTAGTAATTAACATGTGCCAGGCAATGGTGGATTGGAACCCATCCCCA
AGTCCAGCCACCACTGAATAAATCTGATTTCAAAAGTCAAACAGTAGACATTTCCCATTGTCGTTTCTCA
CTCACCACAAGCACCAAATTCACTAGAGTACACTGGTTCCAGAGAGCAGAATCATGTTGGCCTTGGCTAA
TTTCAAAATGCTGTCTTTTACTTTGGTATATGTTGAGGGCTTTTCATAATTTAAAGTGTGTTCTGTTAGC
AAGGCAAAAATTATGAGTCTTAATTCTACAGGCAAATATGCAAAGGAGCCAAAACTGTAAACCCAGCATT
TGGGATGTGAAGACTGGAAGCTAACTCTCATTGAATTCACAAAGTCTTTTATACAATTTCTGTA
Notes:The probe template was PCR amplified from IMAGE:1498883 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1498883 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13389 same embryo
 EMAGE:13388 same embryo
 EurExpress:euxassay_017776 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS