EMAGE mouse gene expression spatial database

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EMAGE:13408

Smc4 structural maintenance of chromosomes 4 ( MGI:1917349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017379_01	euxassay_017379_02	euxassay_017379_03	euxassay_017379_04	euxassay_017379_05

euxassay_017379_06	euxassay_017379_07	euxassay_017379_08	euxassay_017379_09	euxassay_017379_10

euxassay_017379_11	euxassay_017379_12	euxassay_017379_13	euxassay_017379_14	euxassay_017379_15

euxassay_017379_16	euxassay_017379_17	euxassay_017379_18	euxassay_017379_19	euxassay_017379_20

euxassay_017379_21	euxassay_017379_22	euxassay_017379_23	euxassay_017379_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13408_wholemount_strong.wlz

13408_wholemount_moderate.wlz

13408_wholemount_weak.wlz

13408_wholemount_possible.wlz

13408_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13408_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9294
Entity Detected:	Smc4, structural maintenance of chromosomes 4 ( MGI:1917349)
Sequence:	sense strand is shown >T9294 GTTTGGATTTTAGGCGCCATTTTCGAGTGAAGGACCCGGAGCCGAGACACCGGGAGGAGCGAGGCGGGGG GTCGGCCGTGCCACCATTTCAGCCCGGCTCGGAGTGGACCCGCCGACAGCCACCATGCGGCGTAAGGGCA CCAAGCCCTCCACTGCCTGCCACCAGGAGGAAGGGCCGCCGCCCTCCCAGGACGGTGCCCACAGCGACGA AGAGATGGAGCAACCGGCCGGCGAAGCGGAGAGCGCTGCACCCGCAAAGCCTCCAGGTGAGGAGCTGGAT AACAGAAGTTTAGAGGAGATTTTGAATAGTATCCCTCCACCCCCACCTCCAGCCATGGCCAGTGAAGCTG GAGCTCCTCGTCTCATGATAACTCATATTGTAAACCAAAACTTCAAGTCCTATGCTGGAGAGAAAGTTCT GGGACCTTTCCATAAGCGCTTTTCCTGTATTATTGGACCAAATGGCAGTGGAAAATCCAATGTTATTGAT TCCATGCTTTTTGTGTTTGGCTATCGAGCACAAAAAATAAGGTCTAAAAAGCTATCGGTACTGATACATA ATTCTGATGAACACAAGGATATTCAGAGTTGTACTGTAGAAGTTCATTTTCAAAAGATAATTGATAAGGA AGGAGATGATTACGAAGTCCTTCCTAACAGCAACTTCTATGTATCCAGAACCGCCTACAGAGATAGCACT TCTGTCTACCACATAAGTGGGAAGAAGAAGACGTTTAAAGATGTTGGAAATCTCCTTAGAAGCCATGGCA TTGACTTAGACCATAATCGATTTTTAATCTTGCAGGGTGAAGTTGAACAGATTGCTATGATGAAACCCAA AGGCCAGACTGAACATGATGAAGGTATGCTTGAGTATTTAGAAGATATAATTGGCTGTGGACGGCTAAAT GAACCTATTAAAGTCTTGTGTCGGAGAGTTGAAATATTAAATGAACACAGAGGAGAAAAGTTAAATAGAG TGAAAATGGTAGAAAAGGAGAAGGATGCACTAGAGGGAGAGAAAAACATAGCCATTGAGTTCCTTACCCT GGAAAATGAAATGTTTAAGAAAAAGAATCACATCTGTCAATACTACATTTATGATCTGCAGAATAGAATT GCTGAAATAACGACTCAAAAGGAAAAAATTCATGAAGATACTAAAGAAATTACTGAGAAGAGCAATGTGC TTTCCAATGAAATGAAAGCGAAGAATTCAGCTGTAAAGGATGTAGAAAAGAAGCTCAATAAAGTTACAAA ATTTATCGAACAGAATAAAGAAAAATTTACACAACTAGATTTGGAAGATGTTCAAGTTAGGGAAAAATTA AAACATGCTACAAGTAAAGCCAAGAAACTGGAGAAACAACTTCAAAAAGATAAAGAAAAGGTTGAAGAGC TGAAAAGTGTACCTGCCAAGAGTAAGACTGTCATAAATGAGACAACAACTAGAAACAATTCGCTTGAGAA AGAAAGAGAGAAAGAAGAAAAAAAATTAAAAGAGGTCATGGACAGCCTTAAGCAGGAAACACAGGGGCTT CAGAAAGAAAAGGAGATTCAAGAAAAGGAACTTATGGGTTTTAACAAATCAGTAAATGAAGCACGTTCAA AGATGGAAGTTGCTCAGTCAGAACTTGATATCTATCTCAGTCGACATAATACAGCAGTGTCTCAATTAAG TAAGGCGAAGGAAGCCTTAATTACAGCTTCTGAGACTCTCAAGGAGAGGAAAGCTGCAATCAAGGATATA AACACAAAGCTCCCTCAGACGCAACAGGAACTAAAGGAGAAAGAAAAAGAACTTCAGAAACTGACACAAG AAGAAATAAACTTAAAAAGTTTGGTTCATGATCTTTTCCAAAAAGTTGAAGAAGCAAAGAGTTCCTTAGC AATGAATCGAAGTAGGGGGAAAGTACTTGATGCAATAATTCAAGAAAAAAAATCAGGCAGGATCCCAGGA ATATATGGAAGATTGGGAGACTTAGGAGCTATTGATGAAAAATATGACATTGCTATTTCATCCTGTTGCC ATGCATTGGACTACATTGTTGTTGATTCTATTGATACAGCCCAAGAATGTGTAAACTTCCTTAAAAAGCA TAATATTGGGATTGCAACTTTCATAGGTTTGGATAAGATGACAGTGTGGGCCAAAAAAATGAGCAAAATT CAAACTCCTGAAAATACTCCTCGGTTATTTGATCTAGTTAAAGTGAAAAATGAGGAAATCCGCCAAGCCT TTTACTTTGCTTTACGAGATACCTTGGTGGCTAACAATTTGGATCAAGCTACAAGAGTAGCGTACCAAAG AGACAGACGCTGGAGAGTAGTGACTCTGCAGGGACAGATCATAGAACAGTCAGGTACAATGAGTGGCGGT GGAAGCAAGGTGATGAGAGGAAGGATGGGCTCTTCGGTTATCGATGAAATCTCTGTAGAAGAGGTAAATA AGATGGAATCCCAGTTGGAAAGGCATTCTAAACAAGCAATGCAAATCCAAGAACAGAAAGTACAACATGA AGAAGCAGTAGTTAAGTTACGGCACAGTGAACGAGACATGAGAAATACACTGGAAAAGTTCGCTGCAAGC ATCCAGGGCTTATCAGAGCAAGAAGAGTATCTGTGTGTACAAATTAAAGAACTGGAAGCTAACGTTCTTA CTACGGCCCCTGACAGAAAACAACAGAAACTGCTGGAAGAAAATGTCAGTGTTTTCAAAAAAGAATATGA TGCTGTGGCTGAGAAAGCTGGTAAAGTTGAAGCTGAGATTAAACGGCTACACAATACCATCATAGATATC AACAACCGAAAACTCAAGGCCCAGCAAAACAAACTTGACACGATAAATAAGCAGCTTGATGAATGTGCTT CTGCTATTACTAAGGCCCAAGTAGCAATCAAGACTGCTGACAGAAATCTTAAAAAGGCACAGGATTCTGT TTGCCGCACAGAGAAAGAAATAAAAGACACTGAAAAAGAAATAAATGACTTAAAGACAGAGCTAAAAAAT ATTGAAGACAAGGCAGAAGAGGTCATAAACAATACAAAAACTGCAGAGACATCCTTACCAGAAATCCAGA AAGAACATCGTAATCTACTTCAGGAACTAAAAGTTATTCAAGAAAATGAACATGCTCTTCAGAAAGATGC ACTTAGTATTAAGTTGAAACTTGAACAGATAGATGGCCACATTTCTGAACATAACTCAAAAATAAAATAC TGGCAAAAAGAGATTTCAAAAATAAAATTGCATCCTGTTGAAGATAATCCTGTTGAGACAGTAGCAGTTC TAAGCCAGGAGGAGCTTGAGGCAATCAAGAATCCAGAGTCTATAACAAATGAAATTGCACTTTTGGAAGC TCAGTGTCGTGAAATGAAACCAAACCTTGGAGCCATCGCTGAGTATAAAAAAAAGGAAGATTTATATTTG CAAAGAGTAGCCGAACTGGACAAAATTACTTCTGAAAGAGATAATTTTAGACAAGCATATGAAGATCTTC GAAAACAAAGGCTGAATGAATTTATGGCTGGTTTTTACGTAATAACAAATAAACTAAAAGAAAACTACCA GATGCTCACATTGGGAGGAGATGCTGAACTGGAGCTTGTGGACAGTTTAGATCCTTTTTCTGAAGGAATC ATGTTCAGTGTTCGGCCACCTAAGAAAAGTTGGAAGAAGATCTTTAACCTCTCAGGAGGCGAGAAAACCC TTAGTTCCTTGGCCTTAGTGTTTGCTCTTCATCACTACAAGCCCACTCCCCTCTACTTCATGGATGAGAT TGATGCAGCTCTGGATTTTAAAAATGTGTCTATTGTTGCATTTTATATATATGAGCAAACAAAAAATGCC CAGTTCATAATAATCTCTCTTCGAAATAATATGTTTGAGATTTCAGACAGACTTATTGGCATCTATAAAA CATATAATAGTACAAAAAGTGTTGCAGTGAACCCAAAACAAATTGCGTCTAAAGGACTCTGTTGAAGTTG TTCACACTGAAAATTTTCAAATCTAGATTGGGTGTTCTAGTTTTGTCTGTAAAAGACTGTAAGTTGTATA AAATACATTTTTCTAAATTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6841276 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6841276 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13410 same embryo
	EMAGE:13412 same embryo
	EMAGE:13407 same embryo
	EMAGE:13409 same embryo
	EMAGE:13411 same embryo
	EurExpress:euxassay_017379 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source