EMAGE mouse gene expression spatial database

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EMAGE:13411

Elmod2 ELMO domain containing 2 ( MGI:2445165)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017410. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017410_01	euxassay_017410_02	euxassay_017410_03	euxassay_017410_04

euxassay_017410_05	euxassay_017410_06	euxassay_017410_07	euxassay_017410_08	euxassay_017410_09

euxassay_017410_10	euxassay_017410_11	euxassay_017410_12	euxassay_017410_13	euxassay_017410_14

euxassay_017410_15	euxassay_017410_16	euxassay_017410_17	euxassay_017410_18	euxassay_017410_19

euxassay_017410_20	euxassay_017410_21	euxassay_017410_22	euxassay_017410_23	euxassay_017410_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13411_wholemount_strong.wlz

13411_wholemount_moderate.wlz

13411_wholemount_weak.wlz

13411_wholemount_possible.wlz

13411_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13411_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9388
Entity Detected:	Elmod2, ELMO domain containing 2 ( MGI:2445165)
Sequence:	sense strand is shown >T9388 GGAGCGCTCCACGTAGAGCGTCGTTTCCGTTGCCGGTGTTTGCAGCTGGGGAAACCGAGGCAGTGCCAGC TTCCCCCTAGTTCTTCCGCTCCTGTGAGGGAAACATGTTCGTGTCTCTGTGGGAGTTTTTCTATGGACAC TTTTTCCGATTTTGGATGAAATGGCTCTTACGGCAGATGACTGGGAAATGTGAATTGCAGCGAATTTTTG ACACCTATGGAGGGGCACAGAGGACCTACAGAACAGAAAATTCCTTGACATATTCCAAGAATAAGGTCCT ACAGAATGCAACACGAGTTGCCCAGAGTGAACTGGACAGGTGTATAGCGAACATCATGAAGGAAAAGAAC ATCTGCTCCGAGAAGGACACAAGTTTTCAGATCTGCATGCGGACGTGCTTACTGCAGATAACTGGCTATA AACAGCTCTACCACGATGTAGAAAATGTGAGGAAAAAGCCCTATGACTCTGCCAATGCACAGCACGAGAA GATGCTCCTCAAGCTTTGGAGTCTTCTGATGCCAACGAAAAAGTTGACGGCCCGGATCTCCAAGCAGTGG GCAGACATTGGTTTCCAAGGTGACGATCCCAAAACAGACTTCAGAGGCATGGGCATACTCGGACTGATCA ATCTCGTGTATTTCAGTGAAAACTACACCAGCGAAGCGCATCAGATTCTCTCCCGCTCAAATCACCCCAA ATTAGGGTATTCTTATGCAATAGTTGGAATCAACCTTACAGAGATGGCTTATAGCTTACTGAAGAGCGAG GCCCTGAAGCTTCACCTCTACAACTTTGTCCCTGGGGTACCAACGATGGAGCACTTCCACCAGTTTTACT GTTACCTTGTCTATGAATTTGACAAGTTTTGGCTTGAGGAAGAGCCAGAAAGCATTATGTACTTCAACTT GTACAGAGAGAAGTTTCACGAGCGGATCAAAGGACTCTTGATGGATTGCAATGCTGTGCTCACTTTGAAA ACATGAACCATCCGTGTATCTTCACTTTCTACCACATTTTGCACACACAACAGAATTTATATGTTATTAT AGAAATTATCTGATTAACTACACTCATATAATTTCCTACAAAAATACTTCAGAAACTCTATTTAAGAAAG CTAGCGGACAATCAGTGTATGTTTACAGTTGTTTATACACTGTTCTCCAAAGGTCCCTTACCCTTCCAAA GAGCCCTTCCGGAGGTACATGAACTACAGTTTGGAACTTGGGTCTTAGCCTGTTAGTTTAAAAGTATAAA TCAGGTATATTTATAGTTAGCCAATTGATCACAATGTGCACAAGTCTGTTGTCATTTTATGTGTGGTGCT CCCAAGCTGGTGTCTTTTTGAAAATTCTGCAGGGTCATTGTTTGCTCTGGAAGTGGCTTTTGAATGCCCT TTGTCTTTTTATTTCTAGGCAAATCAGCACAGGGTGCCCTCTGAGGCTTTCTTTGTGTCTTTAGCTCTTG TCAAGATGTCCTGTCCTTGGAGCAGAGGGCACATGACTCTGTCAGGGAAGGTGGACAATCATGGCTTCCT TTTGATTTTTCTGGTCACTGACTAAAGAACCGAGACGTGGCAGTACTGATGGCTGCTTCTTACTTATAAG TAGCCCGTGTGGGAGGTTTTATCCTAAGGAGTCTAGGCATTTACTCCAGGTCAGTATTTGTTTTTATCAG CTGTAGAAGGAACCTTCCTCAGAGCCCCCTGGGAGAACCCCGCCTGTGTGTTTAGAAAGGAGGTGCCTCT GCTGAGAAAATCTGGACTGCATTTGAAACATAGTTTCTTTTTCGATAAAACTGTCTTTGAAACTTACACA AGAAGAAAACCGAGAGCTTTTTAGACATCTGTTCCCTATCATTGGTGTGTTTGACCTGCTGTTCACTTTT GCTTGCCCTTGATTTGACTCTGGTGTTTCTGGGTAATATTGTAGGGACCTGGGTTAGTGGGCTGGGACAT GCTACCATAGCCTGGATCATGACTGTGCATTTTTGTATAGCTTTATTCTCCAAAGTCTCAGGAGGGCAGC AAGTATTGTCGAGGATTCTATGTGAGGGGATTCTAATGGGGTGGTTTCCTCTTGGCATGCTTTAGCAATT TGTTCTCAGAGCAAAACAAAAAGAGCCTAAAGATAAGAAAGATGATTGGACTTAGAAGCACTGAGTACGT CCCGTGGAAACCAGTCTACAACATGATAGGTTAACCTAAAACATGGTGCTGTTTTGCACATCTTTTAATA TCAGGTATTTAAAGACTAGCTTGTGTGATAGCGGAAAGTTTGCTGTTTTCTCACAATTTGTTCATGTTTT CTGTCAAGCCTGTCTTCCCACTTGTGGGGTTGGGGGGAGAGAGCAAGCATGAGAAAGTCATGTGCAGTAC GGATGTCCCATCAGAGAGCCACAGCTGGAGTGTTGTATAGCACTGTGTTGCAGAGCATGGGTTCAAATTG CTTTTCCTTAAGGAAGATAAAACTTTTTTTAAAAGTAGCTTTGTTTTATAATACTAGAAAGGATTCAGCT TTTCAGTATTATAGGAATTTAATAATTGATTTCTTATAGGGGACTTGTTGACCCTGGTAATACAGTAATT TCATTTTTGAGAAATCTTTTGTCACCCAGGTTTTAGGATGCATTGTGTACCAAAACCCTAACAAGGAGCC CATGGTTACCATTGTTGTCCACTGTATTATAGTGTGAAGTCCTCCATGGAAAGCAGCTGTGTTAGTCTTT AACTTTTGTGCTAAAAGTACTTATGCATGTATTTTAATCTTTGAGCAGCCTAGATCTTGAACTGATTTAA CTCTTTATCAGCATATGTAACAAAAAGCATACTTGAAGTATGAAAGAGGAAGTAATAATAATAATAATAA GTAATTATTTTCTGGCAGAAAAAAATCTCTAATGATGTTAAGAACATATTGTCATGGTTTGGTTAATGTC ACAGTCCTTAAATCCTCCTTTGTACATTACAGATATTGTTTGTTTTTTTTTGTTTGTTTGTTTATTTGTT AAAATTATCATTAGCCATTTCAGATAATTATATAGTGAACTGATGTGTTAGCCAATTCCAGCTTCAAAGT TCTGATCTTCTGGGAGCTTGACTGTCACTAGTTGTATACTAGAATGGTTCCCTTACTTGCAGCAGACATC AGAACTTCATGCAGTTTTAAAACGCATGTTTAGATGCTCCCCTGTGTCTAGGACTTGTAAAATTACAATG TAGCAATTGGTAGTAATGGCATTGAAGTACAAGAAAGTTACGTTATTTTAGATTAAATCCAGTAGAATCG ATGTGGAGATGGATTCTTGTACATGTGACGATGAAAATATTTCACCAGTGACAAGAGCAGCATAAGAATA GAACCACAACATTTAACAGTTGAGATGCTCTGAAAGACAATCTTTTAAACATGGGAGACACTTGGGTATC ATGGATCCTAGACCAGGCTCTTTGTAAATAAATGTAACAGTACAGGTAGGTATAAAAATGAACTAGCACC ATTTATAGTTCAAAACTCAGACTGTTTTTCTAAAAAATTTCTTCCCTGTTTATTCTTCCTTTCTATTTTA TAGCTACTTAGATTTGTGTTACTTTTTATGGAAATGTATGCAGCGTTTATTCTTCCCTCAGTGTTAGCCA TCATTCATATCATTCTGTCCCTTGAGAATATTCAGCAAAAGGGATTTTGATTACAGGTTTTGCTCAAAGA AAAACAATTTCTTGGGTGTGAAAAGCAGCTCTTCATTTCTGCAGCAGAGGGGAGCAACAGCATAGAAGAT GGAGCTTTGCCTAGGAAGGTGATGGTTTATTATTGTGCTCATAACTGGGGGCCACAGCTGCATCCCCAGC ACAGGTGCTCTACTCTAATCCACAAAGGATGGTGCATCTGGGGAGGCTTTGTCGTCACGTTCATGTGTAC TGCCTCTCCACACAAATGGCTTTTAAATGAAGATGGCTCTTTCACACTCTGAGAAAGATCATGATGTGTA TTTTTGTTTTCTGTCTCCAATTTTTGATGAGATAAAAGTTTAAATGTAGTGGAGGTAGGACCCAACAACC ACATTGACGTTGGTAGAGTAGTTCTATGTTTTGTTAAAAGTACTGACAAATATTTAAGTTGTAGTGTTCC CTGGAGGGAAAGCACCAGTTGGTAACTTAAATTTTTGTCTCTTTTGCATCAAGCATTTGAATTTCATGTA AAAGCAATAGTGTGAGATGTGACGTCCTTTTGTTTGTGATGTTAAGTGAGAGAATAAAATCACAGCTGAA ACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6855187 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6855187 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13410 same embryo
	EMAGE:13412 same embryo
	EMAGE:13407 same embryo
	EMAGE:13408 same embryo
	EMAGE:13409 same embryo
	EurExpress:euxassay_017410 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source