EMAGE mouse gene expression spatial database

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EMAGE:13432

Kdm2b lysine (K)-specific demethylase 2B ( MGI:1354737)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017516_01	euxassay_017516_02	euxassay_017516_03	euxassay_017516_04	euxassay_017516_05

euxassay_017516_06	euxassay_017516_07	euxassay_017516_08	euxassay_017516_09	euxassay_017516_10

euxassay_017516_11	euxassay_017516_12	euxassay_017516_13	euxassay_017516_14	euxassay_017516_15

euxassay_017516_16	euxassay_017516_17	euxassay_017516_18	euxassay_017516_19	euxassay_017516_20

euxassay_017516_21	euxassay_017516_22	euxassay_017516_23	euxassay_017516_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13432_wholemount_strong.wlz

13432_wholemount_moderate.wlz

13432_wholemount_weak.wlz

13432_wholemount_possible.wlz

13432_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13432_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9354
Entity Detected:	Kdm2b, lysine (K)-specific demethylase 2B ( MGI:1354737)
Sequence:	sense strand is shown >T9354 GTTTCCTAGGACTTTGCAAACGGATCTGCTTAAGTGTTGGTGCAAAACTTTGTAGATATCGGCTCTGGCT TGCTCTATTTATTTATTTTTTTGCTTTCTTTTCTTTGGTCTTCCCTCCTTTCCCCCTCCGCCAAAATGCA GGGGGCAGGAGTGTTGACAATTAATTGGTGAACTCTCCTAAAAAAATGGAGGCAGAGAAAGACTCTGGAA GAAGATTGCGTGCGATTGACCGCCAGAGATACGACGAGAACGAAGACTTGTCGGACGTGGAGGAAATTGT CAGCGTCCGTGGCTTCAGCCTGGAGGAGAAGCTACGTAGCCAGTTATACCAGGGGGACTTCGTGCATGCT ATGGAAGGCAAAGATTTTAACTATGAGTACGTACAGAGAGAAGCTCTCAGGGTCCCCCTGGTTTTTCGGG ACAAGGATGGACTAGGGATCAAGATGCCAGACCCTGATTTCACAGTCCGAGACGTCAAACTCCTGGTGGG GAGCCGCCGTTTGGTGGATGTCATGGACGTCAACACCCAGAAGGGTACCGAGATGAGCATGTCCCAGTTT GTGCGCTACTACGAGACACCAGAGGCACAGCGGGATAAACTGTACAACGTCATCAGCCTCGAGTTCAGCC ATACGAAGCTGGAGCATCTGGTCAAGCGTCCCACTGTGGTGGACCTGGTCGACTGGGTGGACAACATGTG GCCTCAGCATCTAAAGGAAAAGCAGACAGAAGCCACCAATGCCCTTGCAGAGATGAAGTACCCCAAAGTG AAAAAGTACTGTCTGATGAGCGTGAAGGGCTGTTTCACTGACTTCCACATTGACTTTGGAGGCACCTCCG TGTGGTACCATGTGTTCCGTGGTGGCAAGATCTTTTGGCTGATCCCTCCAACCCTGCACAACTTGGCTTT GTACGAGGAGTGGGTGCTGTCTGGCAAACAGAGCGACATCTTTCTGGGAGACCGCGTGGAACGCTGCCAA AGAATTGAGCTGAAGCAAGGCTACACCTTTTTCATCCCTTCCGGTTGGATCCATGCGGTTTATACGCCTG TGGACTCTCTGGTGTTCGGCGGGAACATCCTGCATAGCTTCAACGTGCCCATGCAGCTGCGGATCTACGA GATCGAGGACAGGACCCGGGTTCAGCCCAAGTTCCGTTACCCCTTCTACTATGAGATGTGCTGGTATGTC TTGGAGAGATATGTGTACTGTGTGACCCAGCGCTCCTACCTCACTCAGGAATACCAGAGAGAATTAATGC TTATTGATGCCCCAAGAAAAACCAGTGTAGACGGCTTTTCATCCGACTCCTGGCTGGACATGGAGGAGGA GTCCTGCGAGCAGCAGCCACAGGAGGAAGAGGAGGAGGAGGAGGACAAGGAGGAGGAAGGAGATGGTGCA GATAAAACACCCAAGCCACCCACCGATGACCCCACCTCACCCACCAGCACCCCGCCCGAAGACCAGGACA GCACAGGGAAGAAGCCTAAAGCCCCTGCCATACGGTTCCTCAAGAGGACGTTGTCCAATGAGTCAGAGGA AAGTGTCAAGTCGACCTCGATGCCCACGGACGATCCCAAGACGCCCACGGGCTCCCCGGCCACCGAGGTT TCTACCAAGTGGACTCACCTTACCGAATTTGAACTGAAGGGCTTGAAAGCCCTGGTTGAAAAGCTAGAGT CCCTTCCGGAGAATAAGAAGTGTGTCCCTGAGGGAATCGAGGACCCCCAGGCCCTCCTGGAAGGTGTAAA GAATGTACTGAAAGAGCACGTGGATGACGACCCCACCCTGGCCATCACCGGGGTCCCTGTGGTCAGCTGG CCAAAGAAAACTGCAAAGAACCGGGTGGTGGGTCGGCCTAAGGGCAAGTTGGGCCCGGCCTCAGCGGTGA AGTTGGCTGCCAACCGAACAACAGCAGGAGCTCGCAGGCGCCGGACGCGATGCCGCAAGTGCGAGGCCTG CCTGCGGACGGAGTGTGGAGAGTGCCACTTTTGCAAGGACATGAAGAAGTTTGGAGGTCCTGGGCGCATG AAGCAGAGCTGCATCATGCGGCAGTGCATCGCGCCAGTGCTGCCCCACACCGCCGTGTGCCTTGTGTGTG GCGAGGCAGGGAAGGAGGACACAGTGGAAGAGGAAGAAGGCAAGTTTAACCTCATGCTCATGGAATGCTC CATCTGCAACGAGATCATCCACCCTGGATGCCTTAAGATTAAGGAATCGGAGGGTGTGGTCAACGATGAG CTTCCCAACTGCTGGGAGTGTCCGAAGTGTAACCATGCCGGCAAGACCGGGAAACAAAAGCGTGGCCCTG GCTTTAAGTATGCCTCCAACCTGCCTGGCTCCTTGCTCAAGGAGCAGAAGATGAACCGGGACAACAAGGA AGGGCAAGAGCCTGCCAAGCGGAGAAGTGAGTGTGAAGAGGCTCCCCGTCGGAGGTCAGACGAGCACCCC AAAAAGGTGCCTGCAGATGGCATCCTCCGCCGAAAGTCTGATGATGTGCACCTGAGGAGGAAGCGGAAAT ACGAGAAGCCCCAAGAGCTGAGTGGACGCAAGCGAGCCTCGTCGCTTCAAACGTCCCCCGGTTCCTCCTC TCACCTCTCGCCGAGGCCCCCTCTAGGCAGCAGTCTCAGCCCTTGGTGGAGATCCAGTCTCACTTACTTC CAGCAGCAGCTAAAACCTGGCAAAGAAGATAAGCTTTTCAGGAAAAAGCGGCGGTCCTGGAAGAACGCTG AGGATCGTCTGTCACTGGCCAACAAGCCCCTTCGGCGCTTTAAGCAGGAACCGGAGGACGATCTGCCTGA GGCACCTCCTAAGACCCGGGAGAGTGATCAGTCACGTTCCAGCTCACCCACTGCTGGTCCCAGCACTGAG GGAGCTGAAGGCCCAGAAGAGAAGAAAAAGGTGAAGATGCGCCGGAAGCGGCGACTTGTTAACAAGGAGC TGAGCAAAGAGCTAAGCAAGGAGCTCAACCATGAGATCCAAAAGACGGAGAGCACCCTGGCTCACGAGAG CCAGCAGCCCATCAAGTCAGAGCCTGAGAGCGAGAACGACGAGCCCAAGAGGCCCTTAAGCCACTGCGAG CGCCCCCACCGCTTCAGCAAAGGGCTCAACGGCACACCTCGGGAGCTGCGGCACTCGCTGGGACCTGGCC TGCGTAGTCCACCTCGTGTTATGTCCCGGCCCCCGCCCTCTGCATCCCCACCCAAGTGCATCCAGATGGA GCGTCACGTGATCCGGCCACCGCCCATCAGCCCCCCACCTGACTCGCTGCCCCTGGATGATGGAGCAGCC CACGTCATGCATAGGGAGGTGTGGATGGCAGTCTTCAGCTACCTCAGCCACCGAGACCTGTGTGTCTGCA TGCGGGTCTGCAGGACCTGGAACCGCTGGTGCTGCGATAAGCGGTTGTGGACCCGCATCGACCTGAACCG CTGCAAGTCCATCACACCCCTGATGCTGAGCGGTATCATCCGGCGACAGCCTGTCTCCCTTGACCTCAGT TGGACCAACATCTCCAAGAAGCAGCTGAGTTGGCTCATCAACCGGTTGCCTGGGCTCCGAGACTTGGTGC TGTCAGGCTGCTCATGGATCGCTGTCTCAGCCCTCTGTAGCTCCAGTTGTCCATTGCTCCGGACCCTGGA TGTCCAGTGGGTAGAAGGACTAAAGGATGCCCAGATGAGGGATCTCCTGTCTCCACCCACAGACAACAGG CCAGGTCAGATGGACAATCGGAGCAAGCTCCGGAACATTGTAGAGCTGCGCCTAGCTGGCCTGGACATCA CAGATGTCTCCCTGCGGCTCATTATTCGCCATATGCCCCTGCTCTCGAAGCTCCAACTCAGTTACTGTAA CCACATCAATGACCAGTCCATCAACCTGCTCACTGCCGTCGGCACCACCACCCGAGACTCGCTGACAGAG GTCAACCTATCAGACTGTAATAAGGTAACTGACCTGTGCCTGTCCTTCTTCAAACGCTGTGGAAATATCT GTCATATTGACCTGAGGTACTGCAAGCAAGTCACCAAGGAAGGCTGTGAGCAATTCATAGCTGAAATGTC TGTGAGTGTCCAATTTGGGCAAGTGGAAGAGAAACTCCTGCAAAAACTAAGTTAGTTCCAGGACAAGTAT GTAAATATGGAGGGTTGGGGGGAATACAACCAGGAAGGGTGAAAGACTGCAAAATCAAGTGGGCCTATTA TTTCCCTTTGGAATTTGAAACAAGAGACTACAGGACACAGTTCTGTTTTGCAAATCTTGCCAAGGGGGAA GCCATTCAACCACCCATTTTGGGATGAGTGAGCCAGATGTTTTCCTTTGCTCTTTCTGCATCGTAACTGC ACTGCTTTCTGGACCATTTCTAAGGCGGCCTTTAGAAGACATTCCAATTGGAAAGGAAGATGAGCTTTGG AGAAATCCTCTTAGAAGCCTAAGCTTGGTGTTTTTATCAGTAGTTTTTGTTTGGGGCACTTGTTCCTTGC AATGCTGAGGCTTCTGGCGTCAACATGGAGTGGACCACACACACACCATCTGCAGCCGTCGTTGATACAT TTTGTTTAGTTGGTTTTGTTACATCTTACATTATGCAGAACTATTTTTGTATAAATTGTTTAAAAATTAT TTATGGGAGATTTGAATGCATACCAGTGTTTTTATTATTTAGATTGCCAACTGTCCTTCAGGTAGGAGAG TTAACCTGTACTATGTCAGCATAAAAAGCCCATCTCCAGATGTGCCCAGGTGTGACAGGGGGCTATGACC TTCCACTTAAAAAGCATGTTTAACTGGATGCTGAGAGCCTACTGTGTATGTCAAGAAGCTACACAAGCAT GTTGTGGATATGTGTAAAGTATAGTCCAGGTAAAACACTTTGCCAAGTTTCAATAGACTTCACCTCTCCC TCAGACTTGAGCCTTTACACTCAGGAGGGTTTGCTCCAAGCATGAGCTCTTGTATTCTACTTACATAGGT CTAATTTATACCAAGAATCAGGTAGAATAAATGTGCCCACACCCTCTCCTTTGTCCTCCTCCTCTCAAGT GTTCAGTTCTCACTTTAGTTGGTAAGTTCTGCATCCCTTGTTGTTTGGTGGTGTTTCCTAACCTTTCCCT GATGCAGTTTGACCTTTGTTGGATTTATATAAACAATTGTACAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6851421 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6851421 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13433 same embryo
	EurExpress:euxassay_017516 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source