Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13433

Ptbp2 polypyrimidine tract binding protein 2 ( MGI:1860489)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433
"Pseudo-wholemount" of euxassay_017537. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017537_01 euxassay_017537_02 euxassay_017537_03 euxassay_017537_04
EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433
euxassay_017537_05 euxassay_017537_06 euxassay_017537_07 euxassay_017537_08 euxassay_017537_09
EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433
euxassay_017537_10 euxassay_017537_11 euxassay_017537_12 euxassay_017537_13 euxassay_017537_14
EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433
euxassay_017537_15 euxassay_017537_16 euxassay_017537_17 euxassay_017537_18 euxassay_017537_19
EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433 EMAGE:13433
euxassay_017537_20 euxassay_017537_21 euxassay_017537_22 euxassay_017537_23 euxassay_017537_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13433Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13433_wholemount_strong.wlz
13433_wholemount_moderate.wlz
13433_wholemount_weak.wlz
13433_wholemount_possible.wlz
13433_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13433_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6792
Entity Detected:Ptbp2, polypyrimidine tract binding protein 2 ( MGI:1860489)
Sequence:sense strand is shown

>T6792
CGGACGCGTGGGCTCGCTGGCTGCGTGGCTCGGTTCTTGGGAGCGAAGCTTTGTCCGGTTCGGCAATGGA
CGGAATTGTCACTGAGGTTGCTGTTGGTGTGAAGAGAGGATCTGACGAGCTACTCTCAGGCAGTGTTCTC
AGCAGCCCCAACTCTAATATGAGTGGCATGGTAGTTACAGCCAACGGTAACGATAGTAAGAAATTTAAAG
GAGAAGATAAAATGGATGGGGCTCCCTCTCGTGTACTTCACATTCGAAAGTTACCTGGTGAAGTGACTGA
AACAGAAGTTATTGCTTTAGGTTTACCTTTTGGTAAGGTGACTAACATCCTTATGCTGAAAGAAAAAAAC
CAGGCATTTTTGGAACTGGCAACAGAGGAAGCAGCTATTACTATGGTTAATTACTATTCTGCTGTGACAC
CTCATCTTCGTAACCAACCAATTTACATCCAGTACTCCAATCACAAAGAACTAAAGACAGATAATACATT
AAACCAACGTGCGCAAGTAGTTCTTCAAGCTGTGACAGCGGTCCAGACAGCAAATACACCTCTTAGTGGC
ACCACAGTCAGTGAGAGTGCGGTGACTCCAGCCCAGAGTCCAGTACTTAGAATAATTATTGACAATATGT
ACTACCCTGTAACACTTGATGTCCTTCACCAAATATTTTCTAAGTTTGGTGCTGTATTGAAGATAATCAC
ATTTACAAAAAACAACCAGTTTCAGGCTTTGCTCCAGTATGGTGATCCGGTAAACGCTCAACAAGCCAAG
CTAGCCCTAGATGGTCAAAATATTTATAATGCTTGCTGTACCCTAAGGATTGATTTTTCCAAACTTGTGA
ATTTGAATGTAAAATACAACAATGATAAAAGTAGGGATTATACTCGACCTGATCTGCCATCTGGAGACGG
CCAGCCTGCGTTAGACCCAGCCATTGCTGCAGCATTTGCCAAGGAGACATCCCTACTAGCTGTTCCAGGG
GCTCTCAGTCCTTTGGCTATTCCAAATGCTGCTGCAGCAGCTGCTGCCGCTGCCGCTGGCCGAGTGGGCA
TGCCTGGAGTCTCAGCTGGTGGCAATACAGTCCTGTTGGTTAGCAATTTAAATGAAGAGATGGTTACGCC
CCAAAGTCTGTTTACCCTCTTCGGTGTTTATGGAGATGTGCAGCGCGTGAAGATTCTGTACAATAAGAAA
GACAGTGCTCTGATACAGATGGCTGATGGGAACCAGTCCCAGCTCGCCATGAATCATCTTAATGGGCAGA
AGATGTATGGAAAAATTATTCGTGTTACTCTCTCTAAACATCAGACTGTGCAACTACCTCGAGAGGGACT
TGATGATCAAGGGCTAACAAAAGATTTTGGGAATTCACCACTGCACCGTTTTAAAAAACCGGGATCCAAA
AACTTTCAGAACATTTTCCCTCCTTCTGCTACCCTTCACCTGTCTAACATCCCCCCTTCTGTAGCAGAAG
AGGATCTGCGAACTCTGTTTGCCAACACCGGGGGCACTGTGAAAGCATTTAAGTTTTTTCAAGATCACAA
AATGGCTCTTCTTCAGATGGCAACAGTGGAGGAAGCTATTCAGGCTTTGATTGATCTTCATAATTATAAC
CTTGGAGAAAACCATCATCTGAGAGTGTCTTTCTCCAAGTCAACAATCTAAGCACGGGAGATGAAGATGG
CGGGCAGATCCCATTGTTGGTGTCATCACCTATTGACTGTTCAGAAAAGTGGGGACCAGAGTTTGATTTT
TTTCATGCTGTTATCATTCCTTGGTTATAAAATGAAATGGCATATGTAAAGGCAGAGTTGCTAACTGCTG
TGTTTCATCTGTTCAATAGGGAAGCCATTTTTGTCTGTTTAAATTTTTAGTTTAATTTTGCTTTTTTTTT
TTTTCCAACTTAGTTGACATACGTGCCTTACAAAGGAAAACTAGTGTTGCTATTCTGCATTTACTAGGAA
AAAGGAATTGGTTGTTTAGGGCACATCGTTATGTGGGAATTAAAATATGTTTGGGCAGGGGTGTGTCAAA
AGGTTAAGTTTTTATTTCTCCTGCTTGGAACTTTCTTTGAATTATTGGCTTATCACATTTCTTTCTATTT
AATCTAATAAGATACTTGATACTGAGAGTATAAAACAGCATTTTTAGTTTTTACTATCTTAGGCTTTATT
GCTTTTGAAAACATTGGCCTTTTGTATCTCACAAATCTGGTCTAGATTCAGTTATGAATGTAGGCATTAG
TTAAAATTAACAAGATGCAGAGTATTAATTTCTTAAGACAAGTGATTTCTGTAAGTTTGAGCCCTATGTG
GAAAGCATTGTGGAACCTTAACCTTTTTGTACACACTCTTGTGGGACGTGTCATATAAATGTCAGCACTA
AGTAATGTCTTGTTTGTGGCTGAATATTTTTCGTAGATGTTTTTGAAGTTGACATGACTTACGTGCATTT
AAATATATATTGCCATCCTTAGTTTGTAATTAAGATTTGGAAAATGGTTGTGGATTTCTGAGCATGTGCA
GACTGGTCTAGCTAGTTCAGGAACTGGTGCATGTATTTTTCAAAGACAAAGAAAGTGTACTGCGAAAACT
TGCAGGAAGATTAATTTTGTGGCAGTTTTCTAAAACTGACAACCAGGTGGGACCAAAGTTTATGTGCCTT
TAGTCTTAATTTACCTTGCATTGTAATATTCAGTTTTAATAAATCTTCAAAATATTTTGTATTTAGGAAT
AGATCTGACTTTAATAAAAACATGGCTCAGAATCTACAGGTCAAATTTATTTGAACAGTTCTTGTCAATC
TGAATTGTTGATTCTGTTAAATGACCAATACTTTTTGAAATTGATGTACTTAGTTTCAAGATTCATAGAT
TCTGTTATCTATGTAGACAGAATGGTCATGTATATTTTCTATTAGTTGAGTTTTTACATCTTTAGAAATG
TAAAATTCAGTATAGTTTGAAAGCGGCACAATTAAAAATTAATTTTCTAACAAAAAAAAAAAAAAAAAAA
AAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3709255 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709255 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13432 same embryo
 EurExpress:euxassay_017537 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS