EMAGE mouse gene expression spatial database

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EMAGE:13441

Trip12 thyroid hormone receptor interactor 12 ( MGI:1309481)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017791_01	euxassay_017791_02	euxassay_017791_03	euxassay_017791_04	euxassay_017791_05

euxassay_017791_06	euxassay_017791_07	euxassay_017791_08	euxassay_017791_09	euxassay_017791_10

euxassay_017791_11	euxassay_017791_12	euxassay_017791_13	euxassay_017791_14	euxassay_017791_15

euxassay_017791_16	euxassay_017791_17	euxassay_017791_18	euxassay_017791_19	euxassay_017791_20

euxassay_017791_21	euxassay_017791_22	euxassay_017791_23	euxassay_017791_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13441_wholemount_strong.wlz

13441_wholemount_moderate.wlz

13441_wholemount_weak.wlz

13441_wholemount_possible.wlz

13441_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13441_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4305
Entity Detected:	Trip12, thyroid hormone receptor interactor 12 ( MGI:1309481)
Sequence:	sense strand is shown >T4305 GGAACAGCACGTGCCATTCAGAGAAAACCTAATCCCTTAGCCAATTCTAACACTAGTGGATATTCAGAGT TAAAGAAGGATGATGCTCGAGCACAGCTAATGAAAGAAGATCCGGAACTGGCTAAGTCTTTCATTAAGAC CTTGTTTGGTGTTCTCTATGAAGTGTACAGCTCCTCAGCAGGCCCTGCGGTCAGACACAAGTGCCTTAGA GCAATTCTTAGGATAATTTATTTTGCTGATGCTGAACTTCTGAAGGATGTTCTGAAAAATCATGCTGTTT CAAGTCACATTGCTTCAATGCTATCCAGCCAAGACCTGAAGATAGTAGTTGGAGCTCTTCAGATGGCAGA AATCCTAATGCAAAAGTTACCTGATATTTTTAGTGTTTACTTCAGGAGAGAAGGTGTGATGCATCAAGTC AAACATTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCCCCCAAGGCATGTACAAATGGGTCAGGATCCC TGGGATCTACAACACCAGCCAGCAGTGGGACTGCAACGGCAGCAACCAACGCCTCTGCTGATCTGGGGTC ACCTAGCTTGCAGCATAGCAGAGATGATTCTTTGGATCTGAGCCCTCAAGGTCGATTAAGTGATGTTCTC AAGAGAAAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAATATTCCCCTCCAAGAGATGATGACAAAG TAGACAATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCCTTCCTGGCAAGCTTGAATCC AAAAACATGGGGAAGGTTAAGCGCACAGTCCAACAGCAACAATATCGAACCAGCACGAACTGCAGGAGTT AGTGGTCTTGCCAGGGCAGCCTCAAAAGACACCATATCCAATAATAGAGAAAAAATTAAAGGCTGGATTA AGGAGCAGGCACATAAATTTGTTGAGCGTTATTTTAGTTCTGAGAATATGGATGGAAGCAACCCTGCCTT GAATGTCCTTCAGAGACTTTGTGCTGCAACTGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGC CTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCTTCATTTGAAATCCAACATAGTGGGTTTG TGAAACAACTGTTGCTGTATTTGACATCTAAGAATGAAAAGGATGCTGTCGGCAGAGAGATCCGGTTAAA ACGTTTCCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCTGTTGGAAGAGTAGAACCAGTG GGCCATGCACCTTTGCTGGCATTGGTTCACAAGATGAACAACTGTCTCAGCCAAATGGAGCAATTTCCAG TCAAAGTACATGATTTCCCTAGTGGGAACGGAGCTGGAGGAAGCTTTTCTCTCAACAGAGGATCACAAGC TTTAAAATTTTTCAACACACATCAGTTAAAATGTCAGTTACAAAGACACCCAGACTGTGCAAATGTGAAG CAGTGGAAAGGCGGGCCTGTCAAGATTGATCCCCTGGCTCTGGTGCAAGCTATTGAGAGATACCTTGTGG TTAGAGGATATGGAAGAGTAAGAGAAGATGATGAAGATAGTGATGATGATGGGTCAGATGAAGAAATAGA TGAGTCTTTGGCTGCTCAATTCTTAAATTCGGGAAATGTAAGGCACAGGCTACAGTTTTATATTGGAGAA CATTTACTACCATATAACATGACCGTGTATCAGGCAGTGCGGCAATTCAGTGTACAGGCTGAAGATGAAA GAGAATCTACTGATGATGAGAGCAATCCTCTGGGAAGAGCTGGAATTTGGACAAAGACTCATACAATATG GTACAAACCTGTGAGGGAGGATGAAGAAAGTACTAAAGATTGTGTTGGTGGCAAAAGGGGGAGAGCCCAA ACAGCTCCAACAAAAACTTCCCCCAGAAATGCAAAGAAGCATGATGAGTTATGGCATGATGGAGTGTGCC CGTCAGTAGCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATCACCTTTGAAGACCC ATCCTTAGATGTAATACTTCTTTTAAGAGTTTTACATGCCATCAGTCGATATTGGTATTACTTGTATGAT AATGCGATGTGCAAGGAGATTATTCCAACTAGTGAATTTATTAACAGTAAATTAACAGCAAAAGCAAATA GGCAGCTTCAAGACCCTCTAGTAATCATGACAGGAAACATCCCAACATGGCTCACTGAGCTAGGAAAAAC CTGCCCATTTTTCTTTCCTTTTGATACCCGACAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGG GCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGACAGCAGAGTTGCGC CTAGATTGGATAGAAAAAAACGTACTGTGAACAGAGAGGAGCTACTGAAACAAGCTGAGTCTGTGATGCA GGACCTTGGCAGCTCTCGGGCCATGTTAGAAATCCAATATGAAAATGAGGTTGGTACAGGTCTGGGGCCA ACACTGGAGTTTTATGCACTTGTTTCTCAGGAACTACAGAGAGCTGACTTGTGTCTCTGGAGGGGTGAAG AAGTAACTCTTAGCAATCCAAAAGGAAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTT TGCGCTTCCCTTTGGTAGGACAGCTAAGCCAGCTCACATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTA GGAAAATTAATGGCTAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCTCTTGGCCTGCCCTTTTATA AGTGGATGCTGCGGCAGGAAACTTCATTGACATCTCATGATTTATTTGACATTGATCCTGTTGTAGCCAG ATCAGTATATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTGGAGCAGGATAAATCCCAGACCAAA GAGAGTCTACAATATGCATTGGAAACTCTTACGATGAATGGCTGTTCAGTGGAAGATCTGGGATTGGATT TTACGCTGCCTGGGTTTCCCAACATTGAATTGAAGAAAGGAGGGAAGGATATTCCAGTTACTATCCACAA TTTGGAAGAGTACCTGAGATTGGTGATATTCTGGGCACTAAATGAAGGTGTTTGTCGACAGTTTGACTCA TTCAGAGATGGATTTGAATCGGTCTTCCCGCTCTGCCATCTTCAGTACTTCTACCCAGAAGAGCTGGACC AGCTCCTGTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACATTGATGGAATGCTGCAGGCCTGATCA TGGCTATACTCATGACAGTCGGGCTGTGAAGTTCTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAG CAGAGGTTGTTTCTCCAGTTTGTGACTGGTAGCCCACGATTGCCAGTTGGAGGATTTCGGAGTTTAAATC CACCTCTGACAATTGTGCGGAAGACATTTGAATCAACAGAAAACCCAGATGACTTCTTACCATCAGTAAT GACTTGTGTGAACTATCTTAAATTGCCGGACTACTCGAGCATTGACATAATGCGGGACAAACTCTTGATA GCAGCAAGAGAAGGCCAACAGTCGTTCCATCTTTCCTGATCACAACAAGAAATCAGTGTCTGCCTGTTAC AGCAAAAGAAAAAAATCATGATTTCTTTTCTGTGTCACCTGAGTCAAGGAAACATGTTCCTCCCTCTTGT TGTAGGAAAACGGCTTGCAGATTATAATTCGTAATGGCCCTGTGGACTTAAGTGATCAGGCCCTAAAGCA TTGTTGTGATGAGGTTTCTTTAGCAAGTTCTTGTTTAAATTATCATTTATTTGATGAGTGAAGTTTTTAA CTTGCTTTGCTGTGTGAAATTTCAAAGGGATGTTTTTTCAGGCTGGAACAATAAATGTCGCTGTGCAGTT TAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4507222 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4507222 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13444 same embryo
	EMAGE:13443 same embryo
	EMAGE:13442 same embryo
	EMAGE:13445 same embryo
	EurExpress:euxassay_017791 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source