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EMAGE:13466

Cln8 ceroid-lipofuscinosis, neuronal 8 ( MGI:1349447)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13466 EMAGE:13466 EMAGE:13466 EMAGE:13466 EMAGE:13466
"Pseudo-wholemount" of euxassay_017778. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017778_01 euxassay_017778_02 euxassay_017778_03 euxassay_017778_04
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euxassay_017778_05 euxassay_017778_06 euxassay_017778_07 euxassay_017778_08 euxassay_017778_09
EMAGE:13466 EMAGE:13466 EMAGE:13466 EMAGE:13466 EMAGE:13466
euxassay_017778_10 euxassay_017778_11 euxassay_017778_12 euxassay_017778_13 euxassay_017778_14
EMAGE:13466 EMAGE:13466 EMAGE:13466 EMAGE:13466 EMAGE:13466
euxassay_017778_15 euxassay_017778_16 euxassay_017778_17 euxassay_017778_18 euxassay_017778_19
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euxassay_017778_20 euxassay_017778_21 euxassay_017778_22 euxassay_017778_23 euxassay_017778_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13466Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13466_wholemount_strong.wlz
13466_wholemount_moderate.wlz
13466_wholemount_weak.wlz
13466_wholemount_possible.wlz
13466_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13466_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T282
Entity Detected:Cln8, ceroid-lipofuscinosis, neuronal 8 ( MGI:1349447)
Sequence:sense strand is shown

>T282
GGTGGGTGTGCTTCTGTCACTGGGACGCCCTCACCAGCAGCCTGCACCTGCCCCACTGGGCACTGTTCCT
TTTCGGGCTGGCCCTGCTCACGGCCGTCATTAACCCTTACTGGACACACAAGAAGACCCAGCAGCTCCTC
CATCCCGTGGATTGGAACTTTGCACAGGAGGAAGCCAAGGGCAGCAGGCAAGAAAGGACCAATGGCCAGG
TGCCTCGGAAAAAGAGGTTGTAGCGGCGCCCACCCAGGGACTGGGTGGACCATGATGCCGACTGGGGAGT
GTCCAGGGTTGGACAAAGCTCCACAGTGACTCTTTACGTATGGATGGGGACATAGATAAGTTTATATCTA
GGTGAATACTAACTTGTTCCTTACTATGAATTCTAAAGTACCCATGAAGTATTGCTGGGGCCTCGACGTG
CCTGGAAGAGGTCTTTGCCATCATGACTTTCCTCTGAGTCTTCTGGCGTGGCCC
Notes:The probe template was PCR amplified from IMAGE:2655827 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655827 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13465 same embryo
 EMAGE:13468 same embryo
 EMAGE:13470 same embryo
 EMAGE:13467 same embryo
 EMAGE:13469 same embryo
 EurExpress:euxassay_017778 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS