EMAGE mouse gene expression spatial database

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EMAGE:13508

Zfp395 zinc finger protein 395 ( MGI:2682318)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_017764_11	euxassay_017764_12	euxassay_017764_13	euxassay_017764_14	euxassay_017764_15

euxassay_017764_16	euxassay_017764_17	euxassay_017764_18	euxassay_017764_19	euxassay_017764_20

euxassay_017764_21	euxassay_017764_22	euxassay_017764_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13508_wholemount_strong.wlz

13508_wholemount_moderate.wlz

13508_wholemount_weak.wlz

13508_wholemount_possible.wlz

13508_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13508_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8094
Entity Detected:	Zfp395, zinc finger protein 395 ( MGI:2682318)
Sequence:	sense strand is shown >T8094 AAGAGTAATCCGATGGCTGTTGGTGGACAGTGGCTGTGCAGAGCTCAGAGTCACTTGCTATGTTATTTAG CACGGGCCTCACGGCCATGAGAGGAACATGGTCCTTCTGGATTTGGCGCAGTGTGCAGGCATTGGCTCAG TGAGGCAGATGCAGTCTCACCCTCACAGGCGGAGCAGAGTATCCAGCATGCTGTCCCGACGCCTTGGTAA GCGCTCCCTCTTGGGAGCCCGGGTGTTGGGACCTAGTGCCGCTGAAGTACCATCAGGGGCCACCCTGCCT CTGGAGCCACAGATAGAAGTGCCGGAAGGAGCCATGTCCCTGTCCCCACTCACCTCTAAGGACCCTGTGT GCCAGGAGCAGCCCAAGGAGCTCCTCAAAGCTCTGGGAACCTCATGCCACCCACAGGTGGCCTTTCAGCC TGGACAGAAGGTCTGTGTGTGGTATGGAGGTCAGGAGTGCAAGGGCCTGGTGGAGCAGCACAGCTGGGCC GAGGACAAGGTGACCGTCCGGCTGCTGGACCAGAAGTTACAGTGAAGTCTCACCTGATTGTCACCTCCCC ACCCCGTGCTCAGAGCAGCACCAGGAAAGCCCGTGGAGAGGCCAAGAAGTGCCGTAAGGTGTACGGCATC GAGCACCGGGACCAGTGGTGCACAGCCTGCCGGTGGAAGAAGGCCTGCCAGCGCTTCCTGGACTGAGCCT GCCTCACTAGCCCCGCTTCTCACCCTGCCTGGCAGCCGGGAAGCCTCCAGGCCTGCAGCCATCAGCAGAA CACAGGGAGATGATGTGGCGTGGATGTGGGCAGCTGGGGCTCCATTGGCTAAGATAGAACACTTAAAAAC ACTTTTCTCCCCCTTGTTGGGAGTGCTTTATTTTTTAAAAGCAAACCTAAATGAAACTATTTTTCCCCTT AAAATAGGAGAGAGCCAAAATTGACCAAGGGTATTCTGCAGCGAACCGGAGACCAAAGAGTTACCCCTAC CCCTACCCCATTCCACCCTCTCTGGGACTACATATGCATCAAGAGTAGGACAGGATGCTGCCTTGCCTGG TTTTGTTCTGAGCTGGTGAATCCTTTGCTCTTTCAGCTATTCTAGAGTGGCCTGTGAGCAAATGAACTTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTCTCTCTCTCTCCCTTCCTTCCTTCCTTCC TTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTCTTTC TCTCTCTCCCTCCCTCCCTCTCCCTCTCCCCCCCCTTTCTCTCTCTCTCTCTCTCTTTCTTTCTTTCTTT CTTTCTTTTCTTTCAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30054630 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30054630 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13511 same embryo
	EMAGE:13509 same embryo
	EMAGE:13510 same embryo
	EurExpress:euxassay_017764 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source