EMAGE mouse gene expression spatial database

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EMAGE:13509

Nvl nuclear VCP-like ( MGI:1914709)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017582. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017582_01	euxassay_017582_02	euxassay_017582_03	euxassay_017582_04

euxassay_017582_05	euxassay_017582_06	euxassay_017582_07	euxassay_017582_08	euxassay_017582_09

euxassay_017582_10	euxassay_017582_11	euxassay_017582_12	euxassay_017582_13	euxassay_017582_14

euxassay_017582_15	euxassay_017582_16	euxassay_017582_17	euxassay_017582_18	euxassay_017582_19

euxassay_017582_20	euxassay_017582_21	euxassay_017582_22	euxassay_017582_23	euxassay_017582_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13509_wholemount_strong.wlz

13509_wholemount_moderate.wlz

13509_wholemount_weak.wlz

13509_wholemount_possible.wlz

13509_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13509_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6847
Entity Detected:	Nvl, nuclear VCP-like ( MGI:1914709)
Sequence:	sense strand is shown >T6847 TAACAAGAGGCGCCCGTGCGGGGCAGCAGCATCATGAAGCCTAGACCCGGGGTTTTCGTAGACAGGAAAC TGAAGCAGCGGGTCATTCAGTACCTCAGTAGTAACAGATGTGGCAAATATGTGGACACTGGAATCTTAGC ATCCGACTTGCAAAGACTGTACAGTGTAGATTATGGTCGAAGAAAAAGAAATGCTTTCAGGATTCAGGTA GAAAAAGTATTTAGCATAATTAGTAGTGAGAAAGAACTTAAGAATCTTAAAGAACTAGAAGATGGACATC TGGCAAAAAGAGCAAGACAAGATGAAGAGGATGAGTACACTGAAAGCTATTCTGATGATGATTCGAACAT GGAAGATTACCCAGATCCACAGTCAGCAAATCCCATGAACAGCTCCCTGCTCTCTTTGTATCGGAGAGGA AACTCAGAGTCTGTTTCAACCACTCCGAAGTGGGGACAGAGAGAAGCCACCACTTCCACACCATTGCTGA CGTCTAAAACGGGCTCCGTGCCATTGAAGACCCCTGCCCGAGAATCTGAGGGAGGATGGTTTATTGACAA AACTCCCGGTGGAAAGAAAGAAAGTCTTCCCTTGGACCTATCAGATGACCAAAGCAACTCTAAAAAGCAG GATTCTGAGATTCAGATTTTAAAAGACTCTTCTCTTTTGGAAAGTGATAAGAAAAGGAAAGGTAGGGCAA AGGGCAAAGGAAACAAAAGAAAAACAGAGAATCTTCAAGAAGTAGATGGAGAAATAGAAGCTCTTCTGCA AAAGAAAGCCAAAGCAAGGAGTACAGAGTTGCAGATTTCCAATGTGAAGTTTGAAGATGTTGGAGGCAAT GATGCAACATTAAAAGAAGTCTGCAAAATGCTCATACACATGCGCCATCCAGAGGTATACCAGCACCTTG GTGTCGTGCCCCCTCGTGGAGTTCTCCTGCATGGACCACCAGGCTGTGGGAAGACATTACTTGCACACGC AATAGCAGGGGAACTTGACTTGCCGATTCTGAAAGTGGCTGCTCCAGAGATTGTGTCCGGAGTTTCTGGA GAGTCTGAGCAGAAGCTCCGTGAGTTATTTGACCAGGCTGTGTCAAATGCACCATGTATTGTTTTCATTG ATGAAATTGATGCAATTACCCCCAAAAGAGAAGTTGCTTCAAAAGATATGGAACGAAGAATTGTAGCCCA GCTTCTAACCTGCATGGATGATCTGAATAACGTGGCAGCTACAGCTCGGGTCCTAGTTATTGGAGCTACT AACAGGCCAGACTCATTGGATCCTGCTTTGAGACGTGCAGGAAGGTTTGACCGAGAAGTCTGCTTGGGTA TCCCAGATGAGGCAGCCAGAGAAAGAATTCTCCAAACGTTGTGCCGAAAACTGAGGCTTCCGGAAACTTT TAATTTTTGTCACTTAGCACACCTAACACCAGGCTTTGTTGGTGCTGATCTGATGGCTCTTTGCCGAGAG GCGGCCATGTGTGCGGTCAATAGGGTCTTGATGAAACAACAGGCGCAGCAGAAGAAAAAGCCTGAGATTG AAGGTTTGCCCTCTGAAGGAGACCAGGAGAAAAGGCTGGGAGCTGAGCCCACTTCTGAAACACAGGATGA ATTGCAAAGGCTGTTGGGGTTGCTAAGAGACCAAGATCCCCTCTCAGAGGAACAGATGCAAGGACTGTGC ATTGAATTGAACGATTTCATTGTTGCTCTAGCAGAAGTCCAGCCTTCTGCCAAAAGGGAAGGCTTTGTAA CTGTCCCTAATGTGACATGGGCTGATATTGGTGCCCTGGAAGATATTAGAGATGAGCTTATAATGGCAAT ATTGGCACCTGTTCGCAACCCAGACCAGTTCAGAACTCTTGGTTTGGGGACTCCAGCTGGCATCCTCCTA GCTGGTCCTCCTGGGTGTGGAAAAACTCTGCTAGCAAAGGCTGTGGCAAATGAGTCTGGACTAAATTTTA TATCTGTCAAAGGCCCTGAATTATTGAATATGTATGTTGGTGAGAGTGAACGTGCTGTTCGACAAGTTTT TCAGCGAGCCAAGAACTCAGCCCCCTGCGTGATATTTTTTGATGAAGTGGATGCTTTATGTCCTCGGAGA TCAGACCGAGAAACAGGTGCTAGTGTTCGTGTGGTGAATCAGCTGCTTACAGAGATGGATGGCTTAGAAA CACGCCAGCAAGTTTTTATTTTGGCTGCCACCAATAGACCAGATATCATTGACCCTGCCATTCTGCGCCC AGGCCGCCTGGACAAAACGCTCTTTGTGGGTTTGCCTCCCCCAGCAGACCGTGTTGCCATATTAAAGACT ATTACAAAAAATGGCACCAAACCTCCACTGGATGAAGATGTAAATTTGGAAACAATTGCTAATGACCTTC GTTGTAATTGCTATACGGGTGCGGACCTCACTGCTCTGGTACGGGAAGCTTCTCTCTGTGCCCTGAGACA AGAAATAACAGCTCAGAAGAATGGTGTTGGAGCAGGTGAGCTTAAGGTCAGTCATAAGCACTTTGAAGAC GCTTTCAAGAAAGTAAAACCATCCATATCAATAAAGGATCAAGTGATGTATGAAGCTCTGCAGAGGTCCC TCAGTCAGTGATGTTTGCGCTACCTGGCATGGAGGAGGTGTCTAGTGTGTGGAACTCACCACGAATCACA GTCACTCTGGAGGGTCTACTTTCAGCTTGACACCAATGATCCTTTTTTATTTTTTTAAATCAGAACCAGT TATCTCTTTATTTTTTGGAAAGTAAGTTGGCTGTGTTAGTAAAACCACATCTGCTAGTGTGGGCTGATAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964470 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964470 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13511 same embryo
	EMAGE:13508 same embryo
	EMAGE:13510 same embryo
	EurExpress:euxassay_017582 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source