EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13516

Chmp3 charged multivesicular body protein 3 ( MGI:1913950)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017677. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017677_01	euxassay_017677_02	euxassay_017677_03	euxassay_017677_04

euxassay_017677_05	euxassay_017677_06	euxassay_017677_07	euxassay_017677_08	euxassay_017677_09

euxassay_017677_10	euxassay_017677_11	euxassay_017677_12	euxassay_017677_13	euxassay_017677_14

euxassay_017677_15	euxassay_017677_16	euxassay_017677_17	euxassay_017677_18	euxassay_017677_19

euxassay_017677_20	euxassay_017677_21	euxassay_017677_22	euxassay_017677_23	euxassay_017677_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13516_wholemount_strong.wlz

13516_wholemount_moderate.wlz

13516_wholemount_weak.wlz

13516_wholemount_possible.wlz

13516_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13516_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T987
Entity Detected:	Chmp3, charged multivesicular body protein 3 ( MGI:1913950)
Sequence:	sense strand is shown >T987 TCCTCGAGCCTGTTGGCCTACTGGAGGCGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGG AAGGGGTAAGAGGAGTTCAGTTCATCATGGGACTCTTTGGAAAAACCCAGGAGAAGCCTCCCAAAGAGCT GGTTAATGAATGGTCACTGAAGATCAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGAGATATCCAA AGAGAAGAAGAGAAAGTAAAACGGTCTGTGAAAGATGCAGCCAAGAAGGGCCAAAAGGAAGTCTGTGTGG TTCTGGCCAAGGAGATGATCAGGTCAAGGAAAGCCGTGAGCAAGCTCTATGCCTCCAAAGCACACATGAA CTCTGTGCTCATGGGCATGAAGAACCAACTCGCGGTCCTGCGAGTAGCTGGTTCTCTGCAGAAGAGCACA GAAGTGATGAAGGCAATGCAGAGTCTTGTGAAGATCCCAGAAATCCAGGCCACCATGCGGGAGCTGTCCA AAGAGATGATGAAGGCTGGAATCATAGAGGAGATGTTAGAGGATACATTTGAAAGCATGGATGATCAAGA AGAAATGGAAG
Notes:	The probe template was PCR amplified from IMAGE:1972738 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972738 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13518 same embryo
	EMAGE:13517 same embryo
	EMAGE:13519 same embryo
	EurExpress:euxassay_017677 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source