EMAGE mouse gene expression spatial database

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EMAGE:13524

Bclaf1 BCL2-associated transcription factor 1 ( MGI:1917580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017610_01	euxassay_017610_02	euxassay_017610_03	euxassay_017610_04	euxassay_017610_05

euxassay_017610_06	euxassay_017610_07	euxassay_017610_08	euxassay_017610_09	euxassay_017610_10

euxassay_017610_11	euxassay_017610_12	euxassay_017610_13	euxassay_017610_14	euxassay_017610_15

euxassay_017610_16	euxassay_017610_17	euxassay_017610_18	euxassay_017610_19	euxassay_017610_20

euxassay_017610_21	euxassay_017610_22	euxassay_017610_23	euxassay_017610_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13524_wholemount_strong.wlz

13524_wholemount_moderate.wlz

13524_wholemount_weak.wlz

13524_wholemount_possible.wlz

13524_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13524_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4314
Entity Detected:	Bclaf1, BCL2-associated transcription factor 1 ( MGI:1917580)
Sequence:	sense strand is shown >T4314 GGGAAATGGCCGCTGTGTAGTTAGCACGGAGGTAAACCCTGCACGCACATCGTGCTTCGGCGTGTCAGGT ATTTGAATTTAGAGTTTAGTTTCCCATAACATTTTCTACAAGAAGAATCTTGGTAGTGTCTCAAAGACAT CACTGACTTCTGGATCCTGCAGAAAGCCCAAGGTGAAAAGAAATGGGCCGCTCCAATTCTAGATCACATT CTTCAAGATCCAAGTCTAGATCACAGTCTAGTTCTAGATCAAGATCAAGATCACATTCTAGAAAGAAGAG ATACAGGTCTCGTTCCAGAACATATTCGAGGTCTCGTAGTAGAGATCGTATTTATTCTAGAGATTATCGT CGAGATTACAGGAATAATAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGGTATTATC AAGGAGGAGGAGGGAGATACCATCGAGGTGGCTATAGACCTGTCTGGAATAGAAGGCACTCTAGGAGTCC TAGACGAGGTCGGTCACGGTCCAGGAGTCCAAAAAGAAGATCCGTGTCTTCTCAAAGATCCCGAAGCAGA TCTCGCCGGTCATATAGATCCTCTAGGTCTCCAAGATCATCATCATCTCGCTCTTCATCCCCATATAGCA AATCTCCTGTCTCTAAAAGACGAGGGTCTCAGGAAAAACAAACCAAAAAAGCTGAGGGGGAACCCCAAGA AGAGAGTCCTTTGAAAAGCAAATCACAGGAGGAACCAAAGGATACTTTTGAACATGATCCATCTGAATCT ATTGATGAGTTTAATAAATCTGCTACTTCTGGTGATATTTGGCCTGGCCTTTCAGCTTATGATAATAGTC CAAGGTCACCTCATAGTCCTTCACCTATTGCTACACCACCCAGTCAGAGTTCATCATGCTCAGATGCCCC CATGCTTAGTACAGTGCACTCTGCCAAAAATACCCCTTCTCAGCATTCACATTCCATTCAGCACAGTCCT GAGAGGTCTGGATCTGGCTCTGTTGGAAATGGGTCGAGTCGATATAGTCCTTCTCAGAATAGTCCGATTC ATCACATCCCATCACGAAGAAGCCCTGCCAAGACAATCACACCACAGAATGCTCCAAGAGAGGAATCTAG GGGGCGGTCCTCCTTTTATCCTGAAGGAGATCAGGAAACAGCAAAGACAGGAAAGTTTTTAAAAAGGTTC ACAGATGAAGAGTCTAGAGTATTCCTGCTTGATAGGGGTAATATCAGGGATAAAGAGGCTCCGAAGGAGA AAGGGTCAGAGAAAGGCAGGGCAGATGGAGATTGGGATGACCAGGAAGTTCTAGATTACTTTAGTGATAA AGAGTCTGCAAAACAAAAATTCCATGACTCTGAAGGGGATGACACAGAGGAGACAGAGGATTATAGACAG TTTAGGAAATCAGTCCTAGCAGATCAGGGCAAGAGCTTTGCTACTTCATCTCACCGGAATACTGAGGAGG AAGGACCCAAGTACAAGTCCAAAGTTTCATTAAAAGGCAATAGAGAAAGTGATGGATTTAGAGAAGAAAA AAATTATAAACTGAAAGAGACTGCATACATAGTCGAAAGGCCTAGCACTGCAAAGGACAAGCACAAGGAA GAAGACAAAGGCTCTGATAGAATAACAGTGAAGAAAGAAGTGCAGTCACCGGAGCAGGTAAAGTCTGAAA AGCTCAAAGAGCTCTTTGATTACAGTCCCCCTCTACACAAGAGTCTGGATGCACGAGAAAAGTCCATCTT CAGAGAGGAGAGCCCACTGAGGATCAAAATGATAGCCAGTGATTCTCACCGTCCTGAAGTCAAACTCAAA ATGGCACCTGTTCCTCTTGACGATTCCAACAGACCTGCCTCCTTGACTAAAGACAGGCTACTTGCTAGCA CGCTTGTCCATTCTGTCAAGAAAGAGCAAGAATTCCGATCCATCTTTGACCACATTAAGCTGCCTCAGGC CAACAAAAGCACTTCAGAATCATTTATACAACACATTGTATCCTTGGTTCATCATGTGAAAGAACAATAC TTCAAATCACCTGCAGTGACCCTAAACGAGCGGTTCACTTCATACCAGAAAGCTACTGAAGAACATAGTA CCCGGCAAAAGAGCCCCGAGATACACAGGAGGATTGACATCTCTCCCAGCGCTCTGAGGAAGCACACCCG GCTAGCAGGAGAGGAGAGAGGTTTTAAAGAAGAAATTCAGAAGGGAGATAAAAAGTTAAGGTGTGATTCG GCTGACCTTCGGCACGACATTGACCGTCGCCGTAAAGAGAGAAGTAAAGAGCGGGGAGATTCCAAGGGCT CCAGGGAGTCCAGTGGGTCCAGAAAGCAAGAAAAAACACCAAAGGATTACAAGGAGTATAAACCTTACAA AGATGACAGCAAACATAAAGGTAGAGAGCGGGACCATTCCCGGTCATCGTCCTCGTCAGCATCGCCGTCC TCGCCCAGTTCTCGAGAAGAAAAAGAGAGTAAGAAAGAGAGAGAAGAAGAATTTAAAACTCACCACGAGA TGAAAGACTACTCAGGATTTGCAGGAGTTAGCAGACCACGAGGAACCTTTTTTCGAATTAGAGGCAGAGG AAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAACAACTCAAATACCACTTTTCAAAAGAGA CCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGCAAGAAGTACTTCTTGCACGATGACAGAG ATGATGGTGTGGATTACTGGGCCAAAAGAGGAAGAGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTT TAATTTCAAAAAATCAGGTAGCAGTCCAAAATGGACTCATGACAAATACCAAGGAGATGGGATTGTTGAA GATGACGAAGAGACCATGGAAAATAATGAAGAGAAGAAGGACAGACGCAAAGAAGAAAAGGAATAGTCTC AAGATTGCAACAGGGAGCAGAACTTGACACTTACATTTTTTTAAACCTGATTTTTGTTTTCAAATAAGAA TGTAAGCATTTTACTTAAACTGTACTGTTTGCAAGTAGTTTATAGAAATTTTGTTTTAAGTCTTCAAATA CCTTGAAAAAAAATAGACTGTATGTTGAAAATTGTATTGAAATAAAGTAGAAAATGTTCGGTAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4507876 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4507876 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13523 same embryo
	EMAGE:13526 same embryo
	EMAGE:13522 same embryo
	EMAGE:13525 same embryo
	EurExpress:euxassay_017610 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source