EMAGE mouse gene expression spatial database

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EMAGE:13535

Plekhf2 pleckstrin homology domain containing, family F (with FYVE domain) member 2 ( MGI:1919051)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017583. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017583_01	euxassay_017583_02	euxassay_017583_03	euxassay_017583_04

euxassay_017583_05	euxassay_017583_06	euxassay_017583_07	euxassay_017583_08	euxassay_017583_09

euxassay_017583_10	euxassay_017583_11	euxassay_017583_12	euxassay_017583_13	euxassay_017583_14

euxassay_017583_15	euxassay_017583_16	euxassay_017583_17	euxassay_017583_18	euxassay_017583_19

euxassay_017583_20	euxassay_017583_21	euxassay_017583_22	euxassay_017583_23	euxassay_017583_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13535_wholemount_strong.wlz

13535_wholemount_moderate.wlz

13535_wholemount_weak.wlz

13535_wholemount_possible.wlz

13535_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13535_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4256
Entity Detected:	Plekhf2, pleckstrin homology domain containing, family F (with FYVE domain) member 2 ( MGI:1919051)
Sequence:	sense strand is shown >T4256 GGTGAAGTCACCTGCGAGCGGCTGCGCCCGCCGCGTCCGGATCGCGCCGGCCTCGCGGGGCCCCACGCAA CCCTGCGAGGCTGGGGTCGGGGAGCAGGCGGAGTCCGCGCCCGGAGTCGGCGACTAGGAGGACTCGCACC CTCGGCGCCGCCGCCCGCGCGGGGTCGGGTTATTAGCAAAAGATGGTGGATCGCCTGGCAAACAGTGAGG CGAATACTAGACGCATAAGTATTGTGGAGAGCTGTTTTGGAGCCGCTGGTCAGCCCTTGACCATCCCTGG GCGAGTGCTTATTGGAGAAGGAGTGTTGACTAAGTTGTGCAGAAAGAAGCCTAAAGCAAGGCAGTTTTTC TTATTTAATGATATTCTAGTATACGGCAATATTGTTATCCAGAAGAAAAAATACAACAAACAGCATATTA TTCCCTTGGAAAATGTCACCATTGATTCCATCAAAGATGAAGGGGAATTACGGAATGGATGGCTTATTAA GACACCGACTAAGTCGTTTGCAGTTTATGCTGCCACTGCCACGGAGAAGTCAGAGTGGATGAATCACATA AATAAGTGTGTCACTGATTTACTCTCCAAAAGTGGGAAGACGCCCAGCAACGAGCATGCTGCTGTCTGGG TTCCTGACTCTGAGGCCACCGTGTGTATGCGCTGTCAGAAAGCAAAATTCACACCAGTCAATCGGCGGCA CCATTGCCGCAAATGTGGCTTTGTTGTTTGTGGTCCCTGCTCTGAAAAGCGATTTCTTCTTCCCAGCCAG TCTTCTAAGCCTGTGCGGATTTGTGACTTCTGCTATGACCTGCTTTCCACGGGGGACATGGCCGCGTGTC AGCCGACTAGATCAGACTCTTATAGTCAGTCGTTGAAGTCTCCTTTAAATGATGCATCTGATGACGACGA CGATGATGATAGCAGTGACTAAGGATATGCTGTGAAGTATTTAATTGACTGCCTGAGAGTCAGCTTTGGG GGCGCTCTCAGTTTGCTTTAGCCATGACTGCCTGAGAAGTCTTAACCTCTCTGCCTCTCTGTATCCTATG GAAAGCAGCCCCATGCTTTCTGCTCCCCTCTGCACTCTCTCACAGGGACAAACTTGCAGATACATCCGAT AGGGTCTTGGCTTTCTACTGTCCTTATTTCTAGATTATTTCCTTGTAAGTTGGAAAGAATGTTTATTTAA CAGATCTTGTATTACATTTATTTCCCCCTGATGTGGAAAATCTAAAAGCACAGAATGATGGGAACAAGAG TATGATGTGCTTGGTATCTGGCTTTTCCCCAAGCAGTCGATGTCAAGGTTACGACACCAGTGACAGAGCA TGGTTTGGAAATCCTTTATTCTCTTTTCTCAATACCAAGTGGTGCCTTACTGTGGCAGCAGTGTCACTGT CACTGAAATAAACGTAAGCCCTACCTTCTTTCTTGTTTGATTGATTTAGACACACTGGTGCTCTTACAGG TGGAGAATGAGTGTTTGATGGATATAGGTAAGCATATTTATTTGACAGCTATGAGGAACTAAATTTAGAG GTTTCTCCAATCTTATATGAATATTTTTCATAAAATAGTTGTGATTATATTTTTACATCTTTTATGCACC AAATTATAATGGTCACATATGTTTAGCCCGTTGCCACTGTTCGGGATTCGATTTGAAACTCATCACTAGT ATAGAAGTGGCATATCCCACTAGCTTCTACTTTCCTGATATATTTTTACAAATAACTTTATTTGCTTTAC ATTATTTCTAAAAGGAAAAATCTGAGGAAACCCTTATATTTTCCACAGGGTTCCTCTGTGTAGCTTTGCT TATCCTGGAACTCGATTTGTTGACCAGGCTGGCCTTGAATTCTCAAAAATCCATCTGCCTCTGCCTTTGC CTCCCCAGGCACCACTACCACCTGACAAAAATGAAAAGTCTTAAGAAAATCCTCCCAGGAGAACATGGAG TCTTAGAGATCTGGAAGAGATCGGAGTTTGACTAGGTTTCCTGTCTGCAAGTCAATTTTTTTTGTGAAAT TATCTTGATTTACAACGTGGTATTGTATTTTACAGAGTTCATGAAGCCCCAAACAATTTTTATTTTTGTA AAACAATTGTATGTTTAATCTGTTTCTGAAATCATTTTGCAATATATGGAAATAGAGTAGCAGTTGATAT TTCTAAATTGTGACCATTGTCCGAGATTGCTTTGAGAACTGGTAGTTATTCTCTTTATGTTTGGAGGCAG CCATTAGGCTGTAGGCCTGCTTACTGCATAGCACTGGACGTGCCAGGCACATTCTGGCCCCTGGCATGCT TAAGTAACTAGGTTTTCAAATGTGGGAAACCAAGCTGAAAGGTCTGTTTTGTTTTGTTGTTGTTTTGTTT TGGTTTGTTTTTTTTTTTTTTTTCAGAGTTCTTTTCAAACCCAGTTTCCCTTTTTCAAACTTTAAAACCT GCATAATTCTGCAGATGTCCTAGGGTTTGAGTCAGTAACTTGTGAATGAAAGTCATTGGTATTTGGCTTT GTGGTTACAGTACTAATTTTACTTTGCTGCTCAGTTTTGAAAGTGTGTGAGCTACATAGGTCTCCCTGTA GGAAATGTGAAAAAAAGCACAATAGAACCAGGCTAGGGGCTTTGTTCCTGCTTTTTGTTTTGTTTGGTTT TACTATCAGATGGATGGATTTGTGTTTGTCTCAGAGCAGCATATACATGCAGTCAGTAAGCATGTTTGAA AGGACCATAAAAACCAGAGAAATGCTGATGGGAACTTGGAAGTTCAGAAACCAAGGGGAAATGTCCTGAG ATAACATTTGCATTGTCGACCTCAGTTGACTGTTTTTACAATAAAAATATTTTGCAGCTTAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503757 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503757 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13534 same embryo
	EurExpress:euxassay_017583 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source