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EMAGE:13577

4833420G11Rik RIKEN cDNA 4833420G11 gene ( MGI:1918877)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577
"Pseudo-wholemount" of euxassay_017828. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017828_01 euxassay_017828_02 euxassay_017828_03 euxassay_017828_04
EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577
euxassay_017828_05 euxassay_017828_06 euxassay_017828_07 euxassay_017828_08 euxassay_017828_09
EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577
euxassay_017828_10 euxassay_017828_11 euxassay_017828_12 euxassay_017828_13 euxassay_017828_14
EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577
euxassay_017828_15 euxassay_017828_16 euxassay_017828_17 euxassay_017828_18 euxassay_017828_19
EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577 EMAGE:13577
euxassay_017828_20 euxassay_017828_21 euxassay_017828_22 euxassay_017828_23 euxassay_017828_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13577Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13577_wholemount_strong.wlz
13577_wholemount_moderate.wlz
13577_wholemount_weak.wlz
13577_wholemount_possible.wlz
13577_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13577_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40413
Entity Detected:4833420G11Rik, RIKEN cDNA 4833420G11 gene ( MGI:1918877)
Sequence:sense strand is shown

>T40413
AGCAGAGCTTTCCTTACCTCCTGGACAGGTGGCTTTTTAACATCATTGTGAGATGTAACCTCCCATCTGG
GGTAAGGATAAGAATAAGGTAGAGGAGTTTCAGGAGAGACGTGCCTTTGGTTAAGAAGACATGACAAATA
ATCATGAAGACCAGAGTCCGGCACCCAGAAAAGTCAGGCATCCCCCAAATTCCTGTCACTTCAGTTCCAA
GGAATCTGATGTCCTCTTTGGCCTCCACATACACATAGGCAGGCAGGCACAAGAACACACACACACATAC
ACACACGTCTTTTTTTAAGGCAGAGGAAAAGGAAGTTTTAATTTTCAGGTAGCTAATAGATTAAATGAAA
CAGAAGTTGGGAAGGAGTATCGAAGATTAAGTGTTTGTTTTTAAAGCACCATATAAAGCCAGGCATGGTG
GCTCCTGCCTGCAATTACAGCATTTACAAGGCTGAGGAAGGGTTTTTCCAGTCCAGCCAAGGCTATATTA
TACATAAGATCTTTTCTTATATGAAAAAGTACCAGATAGCTAGCATATTTGTATCTTTTGTTAATATATA
TATATGATTACTTATATAGCTAATCACTTTGACGTGTTCTTAAAATTAGAGGGCATGAGATGTATAGTAA
TGGAGGTTCTCTCTGTAGGGATTATCCTCAAATACACAGGAGGCTTGTTGCATGCATAAAATGTTCAGTG
GCTGTAAGACAAGGAGTTGACAATTTACCAACATTTCAGTCAGTAGCTTAGCTGATGCCACAGATACCAG
TGACTCTGGAGAACGTCAAATAGAACTGCTTGCTTATCCATGGTCTGTTAGTGCCTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 153542. Forward Primer - name:153542_F_cDNA_TC1495998, sequence:AGCAGAGCTTTCCTTACCTCCT; Reverse Primer - name:153542_N_SP6_cDNA_TC1495998, sequence:TAGGCACTAACAGACCATGGATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13578 same embryo
 EMAGE:13575 same embryo
 EMAGE:13574 same embryo
 EMAGE:13576 same embryo
 EurExpress:euxassay_017828 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS