EMAGE mouse gene expression spatial database

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EMAGE:13591

Mir434 microRNA 434 ( MGI:3619405)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019189_01	euxassay_019189_02	euxassay_019189_03	euxassay_019189_04

euxassay_019189_05	euxassay_019189_06	euxassay_019189_07	euxassay_019189_08	euxassay_019189_09

euxassay_019189_10	euxassay_019189_11	euxassay_019189_12	euxassay_019189_13	euxassay_019189_14

euxassay_019189_15	euxassay_019189_16	euxassay_019189_17	euxassay_019189_18	euxassay_019189_19

euxassay_019189_20	euxassay_019189_21	euxassay_019189_22	euxassay_019189_23	euxassay_019189_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13591_wholemount_strong.wlz

13591_wholemount_moderate.wlz

13591_wholemount_weak.wlz

13591_wholemount_possible.wlz

13591_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13591_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

weak

regional

weak expression: see section 04 05 06 23 24

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 24

hindlimb digit 2 phalanx

weak

regional

weak expression: see section 24

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 02 24

hindlimb digit 3 phalanx

weak

regional

weak expression: see section 02 24

femur

weak

regional

weak expression: see section 20 24

meckel's cartilage

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 15 16 19 20 21 23 24

orbito-sphenoid

weak

regional

weak expression: see section 05 06 07 08 09 19 20 21 24

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 05 06 24

pelvic girdle skeleton

weak

regional

weak expression: see section 02 03 04 05 06 08 09 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70022
Entity Detected:	Mir434, microRNA 434 ( MGI:3619405)
Sequence:	sense strand is shown >T70022 TTTGAACCATCACTCGACTCCT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-434-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13592 same embryo
	EMAGE:13593 same embryo
	EMAGE:13590 same embryo
	EurExpress:euxassay_019189 same experiment
	MGI:4826284 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source