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EMAGE:13620

Six5 sine oculis-related homeobox 5 homolog (Drosophila) ( MGI:106220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620
"Pseudo-wholemount" of euxassay_019524. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019524_01 euxassay_019524_02 euxassay_019524_03 euxassay_019524_04
EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620
euxassay_019524_05 euxassay_019524_06 euxassay_019524_07 euxassay_019524_08 euxassay_019524_09
EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620
euxassay_019524_10 euxassay_019524_11 euxassay_019524_12 euxassay_019524_13 euxassay_019524_14
EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620
euxassay_019524_15 euxassay_019524_16 euxassay_019524_17 euxassay_019524_18 euxassay_019524_19
EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620 EMAGE:13620
euxassay_019524_20 euxassay_019524_21 euxassay_019524_22 euxassay_019524_23 euxassay_019524_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13620Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13620_wholemount_strong.wlz
13620_wholemount_moderate.wlz
13620_wholemount_weak.wlz
13620_wholemount_possible.wlz
13620_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13620_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55176
Entity Detected:Six5, sine oculis-related homeobox 5 homolog (Drosophila) ( MGI:106220)
Sequence:sense strand is shown

>T55176
GCCCTTGCTACTCACAGGAGGAAGTGCCCCTCAACCACAGCCCAGTCTCCAAGGGGTCAGTGAGGCCAAG
AATTCTCTGGTCCTGGACCCTCAGACAGGAGAGGTTCGACTGGATGAGGCTCAGTCTGAGGCCCCTGAGA
CCAAAGGGGTTCATGGGACTACTGGAGAGGAAATCCCAGGAGCCCTGCCCCAAGTAGTCCCAGGCCCCCC
ACCTGCCTCCACCTTTCCTCTGACCCCGGGAGCTGTGCCTGCTGTGGCTGCTCCTCAGGTTGTACCGCTC
TCCCCTTCTTCTGGGTACCCAACAGGCCTGAGCCCCACCTCCCCACGGCTGAACTTGCCCCAGGTGGTGC
CCACCTCTCAGGTGGTAACCCTGCCTCAGGCTGTGGGGCCACTCCAGCTGTTGGCAGCTGGGCCAGGCAG
TCCTGTGAAGGTGGCAGCTGCAGCGGGGCCTACCAATGTGCACCTGATAAACTCTAGTGTGGGAGTGACT
GCGCTGCAACTTCCCTCGTCCACTGCTCCAGGAAACTTCCTCCTGGCCAACCCTGTGTCTGGTAGCCCCA
TTGTCACTGGGGTAGCTGTGCAGCAGGGCAAGATCATCCTCACTGCCACCTTCCCCACCAGCATGCTCGT
CTCCCAGGTCCTGCCTCCTGCCCCCAGTCTAGCCCTGCCCCTGAAGCAAGAGCCAGCTATCACAGTGCCT
GAAGGAGCTCTCCCAGTGGGCCCCAGCCCCACCCTCCCAGAGGGTCACACTCTGGGGCCAATCTCTACTC
AGCCACTGCCACCTGCTTCTGTTGTCACCTCTGGCACCAGCCTGCCTTTCTCCCCGGACTCCTCTGGCCT
GCTTTCCAGCTTCTCAGCACCCCTACCTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCCCTTGCTACTCACAGGAG; Reverse Primer - name:unspecified, sequence:CAGGTAGGGGTGCTGAGAAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13618 same embryo
 EMAGE:13623 same embryo
 EMAGE:13622 same embryo
 EMAGE:13619 same embryo
 EMAGE:13621 same embryo
 EurExpress:euxassay_019524 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS