EMAGE mouse gene expression spatial database

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EMAGE:13627

Foxf1a forkhead box F1a ( MGI:1347470)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019516. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019516_01	euxassay_019516_02	euxassay_019516_03	euxassay_019516_04

euxassay_019516_05	euxassay_019516_06	euxassay_019516_07	euxassay_019516_08	euxassay_019516_09

euxassay_019516_10	euxassay_019516_11	euxassay_019516_12	euxassay_019516_13	euxassay_019516_14

euxassay_019516_15	euxassay_019516_16	euxassay_019516_17	euxassay_019516_18	euxassay_019516_19

euxassay_019516_20	euxassay_019516_21	euxassay_019516_22	euxassay_019516_23	euxassay_019516_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13627_wholemount_strong.wlz

13627_wholemount_moderate.wlz

13627_wholemount_weak.wlz

13627_wholemount_possible.wlz

13627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 21 moderate expression: see section 09 10 11 18 22 weak expression: see section 08 19 20

vibrissa

weak

regional

weak expression: see section 06 07 21 22 23

esophagus

moderate

regional

moderate expression: see section 15 16 weak expression: see section 17

stomach

strong

regional

strong expression: see section 05 06 07 09 10 moderate expression: see section 03 weak expression: see section 08

hindgut

strong

regional

strong expression: see section 14 15 16 17

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 20

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 weak expression: see section 14 16 17 18

lower jaw molar

moderate

regional

moderate expression: see section 09 21 weak expression: see section 08 19 20

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 weak expression: see section 11 14 16 17 18

upper jaw molar

moderate

regional

moderate expression: see section 09 21 weak expression: see section 08 19 20

bladder

moderate

regional

moderate expression: see section 13 14 15 16 17

urethra of male

moderate

regional

moderate expression: see section 13 14 15 16

lung

strong

regional

strong expression: see section 05 06 07 09 10 11 13 14 15 16 17 18 19 21 22 23 moderate expression: see section 12 20 24 weak expression: see section 08

axial skeleton tail region

strong

regional

strong expression: see section 09 10 moderate expression: see section 11 weak expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55054
Entity Detected:	Foxf1a, forkhead box F1a ( MGI:1347470)
Sequence:	sense strand is shown >T55054 ACGCCGTTTACTCCAGCTCTGCAGCAGCCTGGCCGCCCGCGGCCTCCGCGGCTCTCAACAGCGGGGCCTC CTACATCAAGCAACAGCCTCTGTCCCCTTGCAACCCAGCCGCCAACCCCCTGTCTGGCAGCATCTCCACG CACTCCCTGGAGCAGCCATACCTTCACCAAAACAGTCACAACGGGCCGGCAGAACTGCAAGGCATCCCTC GGTATCACTCACAGTCGCCCAGCATGTGTGACAGAAAGGAGTTTGTCTTCTCTTTCAATGCCATGGCCTC TTCTTCTATGCATACAACAGGCGGAGGATCTTACTATCACCAGCAGGTCACCTACCAAGACATCAAGCCG TGTGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACGCCGTTTACTCCAGCTCT; Reverse Primer - name:unspecified, sequence:ACACACGGCTTGATGTCTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13628 same embryo
	EMAGE:13626 same embryo
	EMAGE:13625 same embryo
	EMAGE:13624 same embryo
	EurExpress:euxassay_019516 same experiment
	MGI:4824904 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source