EMAGE mouse gene expression spatial database

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EMAGE:13628

En1 engrailed 1 ( MGI:95389)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019515. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019515_01	euxassay_019515_02	euxassay_019515_03	euxassay_019515_04

euxassay_019515_05	euxassay_019515_06	euxassay_019515_07	euxassay_019515_08	euxassay_019515_09

euxassay_019515_10	euxassay_019515_11	euxassay_019515_12	euxassay_019515_13	euxassay_019515_14

euxassay_019515_15	euxassay_019515_16	euxassay_019515_17	euxassay_019515_18	euxassay_019515_19

euxassay_019515_20	euxassay_019515_21	euxassay_019515_22	euxassay_019515_23	euxassay_019515_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13628_wholemount_strong.wlz

13628_wholemount_moderate.wlz

13628_wholemount_weak.wlz

13628_wholemount_possible.wlz

13628_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13628_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15 17

submandibular gland primordium

moderate

regional

moderate expression: see section 07 10 11 weak expression: see section 08 09 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 05 06 07 21 22 weak expression: see section 08 09 20

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 15 moderate expression: see section 10 11 12

pons mantle layer

strong

regional

strong expression: see section 09 17 18 19 moderate expression: see section 16 weak expression: see section 11 12

midbrain mantle layer

strong

regional

strong expression: see section 09 13 14 15 16 17 18 19 moderate expression: see section 10 11 12 weak expression: see section 08 20

esophagus

weak

regional

weak expression: see section 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16 17

lower jaw molar

moderate

regional

moderate expression: see section 07 08 19 weak expression: see section 20

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16 17

upper jaw molar

moderate

regional

moderate expression: see section 07 08 19 weak expression: see section 20

metanephros

weak

regional

weak expression: see section 09 10 11 18 19 20 21 22 23

lung

weak

regional

weak expression: see section 05 06 07 09 10 11 12 13 15 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55042
Entity Detected:	En1, engrailed 1 ( MGI:95389)
Sequence:	sense strand is shown >T55042 CGAGCAAGAGAAAGCAATCCTCCGAGTGGACATTCACATAGGAACAAAACGGTTTTTGAAACGGGAGTAA GACTCGGACAGGTGCTATGGGGGAAAAATAAACATCTATTCTCTAACTCACTGTATAAGATGAAACTGCG AATTCCTTAAAGCTCTATCTAGCCAAACTGCTTTCGACCGTGTATATATTTAATTTCAGCAGCCCAGTTT CGTTTTCGTTGCTCTTTTCCTTTGAATGTTTTTGCTTCTCTGGGTACCTCCTGCACCCCCAACGCTGGCC CTGGTTTCTCTGGGACTTTTCTTTGTGTGAGTGTGAGTGTGTTTCCTTGTGTGTCTGCCCCTCGCCTCTT CTCTACCCACCCAGGATTCTTCTATTGGTCTTGTCTATCCCTCCCGTAAATCCCCTTCCTTTTCTGGAGA CTCCTTGAGAAATACAACCCCACAGACTGCGAGACTGAACCGCCG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CGAGCAAGAGAAAGCAATCC; Reverse Primer - name:unspecified, sequence:GGCGGTTCAGTCTCGTAGTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13627 same embryo
	EMAGE:13626 same embryo
	EMAGE:13625 same embryo
	EMAGE:13624 same embryo
	EurExpress:euxassay_019515 same experiment
	MGI:4824549 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source