EMAGE mouse gene expression spatial database

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EMAGE:13639

Slc23a2 solute carrier family 23 (nucleobase transporters), member 2 ( MGI:1859682)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019254. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019254_01	euxassay_019254_02	euxassay_019254_03	euxassay_019254_04

euxassay_019254_05	euxassay_019254_06	euxassay_019254_07	euxassay_019254_08	euxassay_019254_09

euxassay_019254_10	euxassay_019254_11	euxassay_019254_12	euxassay_019254_13	euxassay_019254_14

euxassay_019254_15	euxassay_019254_16	euxassay_019254_17	euxassay_019254_18	euxassay_019254_19

euxassay_019254_20	euxassay_019254_21	euxassay_019254_22	euxassay_019254_23	euxassay_019254_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13639_wholemount_strong.wlz

13639_wholemount_moderate.wlz

13639_wholemount_weak.wlz

13639_wholemount_possible.wlz

13639_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13639_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 09 10 11 18 19 20 21 moderate expression: see section 12

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 13 14 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2372
Entity Detected:	Slc23a2, solute carrier family 23 (nucleobase transporters), member 2 ( MGI:1859682)
Sequence:	sense strand is shown >T2372 GGCCTCGAGGCCAGATTCGGATCCTTGGTGCATACCTGAGCATGCCCAGTGCATGTTGGGTCCATGCACA ACTCCTGGACCCGAGCTTTCACCAAGCTCTGGCCTCCAGGGAGCTGTGTCTTTCTCTGTCCCATTAAGAT TTGGGCACAGATAAGACATCTTCCACTTTCCATCCTTCCTACAGTTAGAGCCCCAGTAGTGCGAGAAGGC CCCATATATGGCAGCATTCAGCTCTCACCCAACACTTGTGCTGGGCTTCCCTTCAGCAGCCTCCCAGACC TCCCCATTCTTCCAGGATGCTGTCTGCCAGGTCACCACTCTGATACCCAGCGTTCTCTGGTTGCTCTCTG TTTCCAGTGATTAAACACCCATAACCAACGTTGCTGTCTGCTGCCACTGACAGTCCAGTGAATCCTTTTC TGTCTGTTCCTAACAACTTTTATTTGACACATTAAAAAATTTCTGACTGTCCTTAAGACAGTAGAGATGT CTCTCTTTTATTTGAGTTTGTGACTGACTTTGCCTATATATCTTTCTGGGGTTTTGTTTGTGTTTAAT
Notes:	The probe template was PCR amplified from IMAGE:1181041 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1181041 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13640 same embryo
	EMAGE:13641 same embryo
	EMAGE:13637 same embryo
	EMAGE:13638 same embryo
	EurExpress:euxassay_019254 same experiment
	MGI:4828141 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source