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EMAGE:13651

Mir384 microRNA 384 ( MGI:3619394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13651 EMAGE:13651 EMAGE:13651 EMAGE:13651 EMAGE:13651
"Pseudo-wholemount" of euxassay_019166. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019166_01 euxassay_019166_02 euxassay_019166_03 euxassay_019166_04
EMAGE:13651 EMAGE:13651 EMAGE:13651 EMAGE:13651 EMAGE:13651
euxassay_019166_05 euxassay_019166_06 euxassay_019166_07 euxassay_019166_08 euxassay_019166_09
EMAGE:13651 EMAGE:13651 EMAGE:13651 EMAGE:13651 EMAGE:13651
euxassay_019166_10 euxassay_019166_11 euxassay_019166_12 euxassay_019166_13 euxassay_019166_14
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euxassay_019166_15 euxassay_019166_16 euxassay_019166_17 euxassay_019166_18 euxassay_019166_19
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euxassay_019166_20 euxassay_019166_21 euxassay_019166_22 euxassay_019166_23 euxassay_019166_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13651Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13651_wholemount_strong.wlz
13651_wholemount_moderate.wlz
13651_wholemount_weak.wlz
13651_wholemount_possible.wlz
13651_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13651_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70009
Entity Detected:Mir384, microRNA 384 ( MGI:3619394)
Sequence:sense strand is shown

>T70009
TGTAAACAATTCCTAGGCAATGT
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-384-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13652 same embryo
 EMAGE:13650 same embryo
 EurExpress:euxassay_019166 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS