EMAGE mouse gene expression spatial database

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EMAGE:13690

Znhit3 zinc finger, HIT type 3 ( MGI:3051596)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019512. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019512_01	euxassay_019512_02	euxassay_019512_03	euxassay_019512_04

euxassay_019512_05	euxassay_019512_06	euxassay_019512_07	euxassay_019512_08	euxassay_019512_09

euxassay_019512_10	euxassay_019512_11	euxassay_019512_12	euxassay_019512_13	euxassay_019512_14

euxassay_019512_15	euxassay_019512_16	euxassay_019512_17	euxassay_019512_18	euxassay_019512_19

euxassay_019512_20	euxassay_019512_21	euxassay_019512_22	euxassay_019512_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13690_wholemount_strong.wlz

13690_wholemount_moderate.wlz

13690_wholemount_weak.wlz

13690_wholemount_possible.wlz

13690_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13690_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 12 13 14 15 16

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 17 18 19 20 21

pancreas

weak

regional

weak expression: see section 09 10 11 12 16 17 18

vibrissa

weak

regional

weak expression: see section 06 07 20 21 22

liver

moderate

regional

moderate expression: see section 01 02 03 05 weak expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55232
Entity Detected:	Znhit3, zinc finger, HIT type 3 ( MGI:3051596)
Sequence:	sense strand is shown >T55232 ATGGCGTCGCTGAATTGTAGGACTGCGGTCTGTGTGGTCTGTTTGGAGAAGCCGAAATACCGTTGCCCGA CTTGCCGCGTGCCCTACTGTTCGGTCCCCTGTTTTCAGAAGCACAAAGAGCAGTGCAGCTCTGAAGCCCG ACCTGTTGAGAAGAGAAGAGCAGGGCCTCCTGTGAGGTCTGAGGAGAGCAAAGATGATGACTCCTCCGTA GCTGATTTCCTCAACAGTGATGAGGAAGAAGACAGAGTGTCTCTGCAGAATTTAAAGAATCTAGGTGAAT CGGAAACTTTAAGAAGCTTGCTGCTGAACCCACACCTGAGGCAGTTGATGATTAGCCTCGATCAGGGTGA CAACAAAGCAAAGCTGATGCGAGCCTGTATGCAGGAGCCCCTTTTCGTGGAGTTTGCAGACTGCTGTTTA GGAATCGTGGAACCATCCCAGAAGAGGGATTCCTAAGACTGGATGTGTTCTCTGCTCAAGTTACCTGCTG CTGCTCCAAGAGGTGGCTGTTTTCAAGGCCACCATACGCAGCATGCACACGTGGGTCTTCCTCTACCACA TGGCTCGCTGCAGCAACTTCATGAAGAGGAAACTTGTAGCCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATGGCGTCGCTGAATTGTAG; Reverse Primer - name:unspecified, sequence:GGGCTACAAGTTTCCTCTTCA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13693 same embryo
	EMAGE:13694 same embryo
	EMAGE:13692 same embryo
	EMAGE:13691 same embryo
	EurExpress:euxassay_019512 same experiment
	MGI:4829362 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source