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EMAGE:13693

Atf5 activating transcription factor 5 ( MGI:2141857)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693
"Pseudo-wholemount" of euxassay_019493. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019493_01 euxassay_019493_02 euxassay_019493_03 euxassay_019493_04
EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693
euxassay_019493_05 euxassay_019493_06 euxassay_019493_07 euxassay_019493_08 euxassay_019493_09
EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693
euxassay_019493_10 euxassay_019493_11 euxassay_019493_12 euxassay_019493_13 euxassay_019493_14
EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693
euxassay_019493_15 euxassay_019493_16 euxassay_019493_17 euxassay_019493_18 euxassay_019493_19
EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693 EMAGE:13693
euxassay_019493_20 euxassay_019493_21 euxassay_019493_22 euxassay_019493_23 euxassay_019493_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13693Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13693_wholemount_strong.wlz
13693_wholemount_moderate.wlz
13693_wholemount_weak.wlz
13693_wholemount_possible.wlz
13693_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13693_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
axial musculature
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 07 23 24
adrenal medulla
moderate moderate
regionalmoderate expression: see section 10 11 19 21 weak expression: see section 12 18
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 09 10 21 weak expression: see section 07 08 18 19 20
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 21 22 23 weak expression: see section 08 20
hypothalamus mantle layer
strong strong
regionalstrong expression: see section 10 11 15 moderate expression: see section 16
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 16 17 weak expression: see section 12
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02 12 14 24
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 22 23
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 19
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 05 06 07 08 09 18 19 21 22 23 weak expression: see section 04 20
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 13 17 18 19 weak expression: see section 12
neural retina
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 23 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
vomeronasal organ
strong strong
regionalstrong expression: see section 12 13 15
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 08 09 10 11 13 14 15 17 18 19 20 21 22 23 weak expression: see section 04 12 16 24
lung
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55008
Entity Detected:Atf5, activating transcription factor 5 ( MGI:2141857)
Sequence:sense strand is shown

>T55008
GCAGCACCTAGGGTACAGGTCCAGGCTGGAGAGGGAGGTCTCGTGTACGTCTGTCTCCAGCGTCACACTA
CCCCTCCATTCCACTTTCCTATCCAGTCCATCTCTAGGCTTCCCCTCTGCCTAACCCACTGTGGGCTATT
TGGCAGAGTCAGTTTCTGTACCCCTTCAGTGCAACTGAGAAAGCAAGGCCCTCAGGGAACTGGAGGAGGA
GCCAGCCTGATCCCTGGCCTCTGGAGTCTTAAAGCAAGTGTGTTTTTGCAGGTAGTCCTAGTTGGGTGTA
GGGGGAGGCTGCCAGGCCTGGCATGAACATAAAGCAGGAAGCCACTCTGGGTGATGGCAGATGAGAACCT
AGTCAGGGAGGGAGGGTTTTAAGGAGGGGACTTGGAACACAAGTGAGAAGCCAGTCAAGAAAAGGGAGCC
AGGACCTCAGAATTTATACTGGCCTAGGGGACAGGAAGGGGTGATTGTAGTTGGTAACTAGGGGTCATTT
TAGCTCTGTGAGAGAATCAGTGTTTTGTGAGGGTGTGGGGGAAGGGTTGCATCTAGTGGAGGCTGATCCT
CTAGGGGTTATGGGCACAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCAGCACCTAGGGTACAGGT; Reverse Primer - name:unspecified, sequence:TTGTGCCCATAACCCCTAGA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13694 same embryo
 EMAGE:13692 same embryo
 EMAGE:13691 same embryo
 EMAGE:13690 same embryo
 EurExpress:euxassay_019493 same experiment
 MGI:4823292 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS