Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13703

Amy2a3 amylase 2a3 ( MGI:3714985)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703
"Pseudo-wholemount" of euxassay_019249. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019249_01 euxassay_019249_02 euxassay_019249_03 euxassay_019249_04
EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703
euxassay_019249_05 euxassay_019249_06 euxassay_019249_07 euxassay_019249_08 euxassay_019249_09
EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703
euxassay_019249_10 euxassay_019249_11 euxassay_019249_12 euxassay_019249_13 euxassay_019249_14
EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703
euxassay_019249_15 euxassay_019249_16 euxassay_019249_17 euxassay_019249_18 euxassay_019249_19
EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703 EMAGE:13703
euxassay_019249_20 euxassay_019249_21 euxassay_019249_22 euxassay_019249_23 euxassay_019249_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13703Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13703_wholemount_strong.wlz
13703_wholemount_moderate.wlz
13703_wholemount_weak.wlz
13703_wholemount_possible.wlz
13703_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13703_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pancreas
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 10 17 18 weak expression: see section 09
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18 19 weak expression: see section 08 09
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 13 14 15 16 17 18 19 20 21
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 12 13 14 15 16 17 18 19
pons marginal layer
moderate moderate
regionalmoderate expression: see section 10 11
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 21 22 weak expression: see section 04 20
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 09 19 weak expression: see section 20
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 05 06 07 09 17 18 19 21 22 23 weak expression: see section 04 20
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 09 18 19
ventral grey horn
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17 18
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 10 11 13 17 18 19 weak expression: see section 12 16 20
neural retina
weak weak
regionalweak expression: see section 01 02 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8164
Entity Detected:Amy2a3, amylase 2a3 ( MGI:3714985)
Sequence:sense strand is shown

>T8164
GCAAAATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCCACATACTTC
AGATGGGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCTGGGTTGATATTGCCAAGGAATGTGAGCGA
TACTTAGCTCCTAAGGGATTTGGAGGGGTGCAGGTCTCTCCACCCAATGAAAACGTTGTAGTTCATAACC
CATCAAGACCTTGGTGGGAAAGATACCAACCAATCAGCTATAAAATCTGCACAAGGTCTGGAAATGAAGA
TGAATTCAGAGACATGGTGACAAGGTGCAACAATGTTGGTGTCCGTATTTATGTGGATGCTGTCATTAAC
CACATGTGTGGCGCAGGCAATCCTGCAGGAACAAGCAGTACCTGTGGAAGTTACCTCAATCCAAATAACA
GGGAATTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGGAGAAATTGATAA
CTACAATGATGCTTATCAGGTCAGAAATTGTCGTCTGACTGGCCTTCTGGATCTTGCACTTGAGAAAGAT
TATGTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGACATTGGAGTAGCAGGGTTCAGACTTG
ATGCTGCTAAGCACATGTGGCCTGGAGACATAAAGGCAGTTTTGGACAAACTGCATAATCTCAATACAAA
ATGGTTCTCCCAAGGAAGCAGACCTTTCATTTTCCAAGAGGTCATTGATCTGGGTGGTGAGGCAATTAAA
GGTAGTGAGTACTTTGGAAATGGCCGTGTGACAGAATTCAAGTATGGTGCAAAACTTGGCACAGTTATCC
GCAAGTGGAATGGCGAGAAGATGTCCTATTTAAAGAACTGGGGAGAAGGTTGGGGTTTGGTGCCTTCTGA
CAGAGCCCTTGTGTTTGTGGACAACCATGATAATCAGCGAGGACATGGTGCTGGAGGATCATCCATCCTG
ACATTCTGGGATGCTAGAATGTATAAAATGGCTGTCGGATTTATGTTGGCTCATCCTTATGGATTCACAA
GAGTAATGTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACC
ACCCAATAACAATGGAGTAACAAAAGAAGTGACCATTAATGCAGACACTACTTGTGGCAATGACTGGGTC
TGTGAACACAGATGGCGTCAAATCAGGAACATGGTTGCCTTCAGGAATGTGGTCAATGGTCAGCCTTTTT
CAAACTGGTGGGATAATAACAGCAACCAAGTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTCTTTAA
CAATGATGACTGGGCTTTGTCAGCCACTTTACAGACTGGTCTTCCTGCTGGCACATACTGTGATGTCATC
TCTGGAGATAAGGTCGATGGCAATTGCACTGGACTTAGAGTGAATGTTGGCAGTGATGGCAAAGCTCACT
TTTCCATTAGTAACTCTGCTGAGGACCCATTTATTGCAATCCATGCTGACTCAAAATTGTAAGAATCTAT
ATTAAAGAGATTTGGATTAAGCATCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6433956 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6433956 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13700 same assay
 EMAGE:13701 same assay
 EMAGE:13702 same assay
 EurExpress:euxassay_019249 same experiment
 EMAGE:13706 same embryo
 EMAGE:13705 same embryo
 EMAGE:13707 same embryo
 EMAGE:13704 same embryo
 EMAGE:31607 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS