EMAGE mouse gene expression spatial database

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EMAGE:13710

Atf6b activating transcription factor 6 beta ( MGI:105121)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019533. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019533_01	euxassay_019533_02	euxassay_019533_03	euxassay_019533_04

euxassay_019533_05	euxassay_019533_06	euxassay_019533_07	euxassay_019533_08	euxassay_019533_09

euxassay_019533_10	euxassay_019533_11	euxassay_019533_12	euxassay_019533_13	euxassay_019533_14

euxassay_019533_15	euxassay_019533_16	euxassay_019533_17	euxassay_019533_18	euxassay_019533_19

euxassay_019533_20	euxassay_019533_21	euxassay_019533_22	euxassay_019533_23	euxassay_019533_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13710_wholemount_strong.wlz

13710_wholemount_moderate.wlz

13710_wholemount_weak.wlz

13710_wholemount_possible.wlz

13710_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13710_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 17 18 weak expression: see section 10 16

vibrissa

strong

regional

strong expression: see section 20 21 moderate expression: see section 04 weak expression: see section 05 06 19 22

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 17 weak expression: see section 09 10 11 16

pons mantle layer

weak

regional

weak expression: see section 08 18

ventral grey horn

moderate

regional

moderate expression: see section 12 weak expression: see section 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 11 12 18 19 weak expression: see section 13

metanephros

weak

regional

weak expression: see section 09 10 11 18 19 20 21

lung

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 06 07 08 12 13 14 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55026
Entity Detected:	Atf6b, activating transcription factor 6 beta ( MGI:105121)
Sequence:	sense strand is shown >T55026 CCTGCTCGTCCTCCTCCCTCAGCTCAGAGTCCTCACATCTTTCCACAGAGCCCCCCAGCCAGGTCCCTGG TGTAGGCGAGGTGCTGCATGTGAAGATGGAGTCCCTGGCACCCCCACTCTGCCTGCTGGGGGATGATCCA GCATCCCCCTTTGAAACGGTCCAGATCACTGTGGGCTCTGCCTCTGATGATCTTTCAGATATCCAGACCA AGCTGGAACCTGCCTCTCCGTCTTCTTCTGTCCACTCTGAGGCCTCCTTGCTGTCAGCAGACTCTCCCAG TCAGCCTTTTATAGGAGAGGAGGTTCTGGAAGTGAAGACAGAGTCTCCGTCCCCTCCAGGGTGCCTCCTG TGGGATGTCCCAGCCTCTTCGCTCGGAGCTGTCCAGATCAGCATGGGTCCATCCCCTGATAGTTCCTCAG GGAAAGCTCCGGCCACTCGGAAGCCTCCACTGCAGCCCAAGCCTGTGGTACTAACCACAGTTCCGGTGCC ACCTAGAGCTGGGCCTACCAGCGCTGCCGTCCTCCTGCAACCCCTGGTCCAGCAGCCTGCGGTGTCCCCA GTGGTCCTCATCCAAGGTGCTATCCGAGTCCAGCCTGAAGGGCCAGCTCCCGCAGCTCCCCGGCCTGAGA GGAAGAGCATTGTTCCAGCCCCTATGCCGGGGAACTCCTGCCCGCCTGAAGTGGATGCAAAGCTGTTGAA GCGGCAGCAGCGGATGATCAAGAATCGAGAGTCGGCCTGCCAGTCCCGCCGCAAGAAGAAAGAGTACCTG CAAGGCCTGGAGGCCCGGCTGCAGGCTGTGCTGGCCGACAACCAGCAGCTGCGCAGGGAGAACGCTGCCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCTGCTCGTCCTCCTCCCTCAGCTCA; Reverse Primer - name:unspecified, sequence:GGGCAGCGTTCTCCCTGCGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13709 same embryo
	EMAGE:13711 same embryo
	EMAGE:13708 same embryo
	EurExpress:euxassay_019533 same experiment
	MGI:4823293 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source