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EMAGE:13712

Med14 mediator complex subunit 14 ( MGI:1349442)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712
"Pseudo-wholemount" of euxassay_019541. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019541_01 euxassay_019541_02 euxassay_019541_03 euxassay_019541_04
EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712
euxassay_019541_05 euxassay_019541_06 euxassay_019541_07 euxassay_019541_08 euxassay_019541_09
EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712
euxassay_019541_10 euxassay_019541_11 euxassay_019541_12 euxassay_019541_13 euxassay_019541_14
EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712
euxassay_019541_15 euxassay_019541_16 euxassay_019541_17 euxassay_019541_18 euxassay_019541_19
EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712 EMAGE:13712
euxassay_019541_20 euxassay_019541_21 euxassay_019541_22 euxassay_019541_23 euxassay_019541_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13712Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13712_wholemount_strong.wlz
13712_wholemount_moderate.wlz
13712_wholemount_weak.wlz
13712_wholemount_possible.wlz
13712_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13712_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 18 19 weak expression: see section 04 20 21 22 23 24
thymus primordium
weak weak
regionalweak expression: see section 11 12 13 14 15
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 16 17 18 weak expression: see section 08 09
vibrissa
moderate moderate
regionalmoderate expression: see section 03 04 05 06 19 20 21 weak expression: see section 02
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 23 weak expression: see section 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 13 weak expression: see section 07 08 09 14 15 16 17 18
heart valve
moderate moderate
regionalmoderate expression: see section 09 10 13 weak expression: see section 11 12
esophagus
moderate moderate
regionalmoderate expression: see section 11 12 13
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 11 14 15
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 18 19
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 11 14 15
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 18 19
lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 16 18 19 21 weak expression: see section 13 14 15 17 20
clavicle
moderate moderate
regionalmoderate expression: see section 09 10 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55104
Entity Detected:Med14, mediator complex subunit 14 ( MGI:1349442)
Sequence:sense strand is shown

>T55104
CAATCATCCACCCACATCAGACTGGCCTTCAGGAACATGTACTGCATTGACATATACTGCCGTAGTCGAG
GCGTCGTGGCAATACGGGACGGTGCCTATAGTCTTTTTGATAACAGCAAGTTAGTTGAAGGCTTCTATCC
TGCACCAGGATTAAAGACTTTCCTGAACATGTTTGTTGATAGCAATCAAGATGCTCGGAGAAGATCTGTA
AATGAGGATGATAACCCTCCTTCTCCTATAGGAGGTGACATGATGGACTCTTTAATATCACAGCTCCAGC
CACCTCAGCAACAGCCATTTCCAAAGCAGCCAGGGACATCAGGCGCTTACCCTCTTACTTCACCCCCTAC
ATCCTATCACAGCACAGTTAATCAGTCCCCCTCTATGATGCACACACAGTCTCCAGGAAATCTGCATGCT
GCCAGCTCCCCCAGTGGGGCTTTGAGAGCCCCATCACCAGCGTCATTTGTTCCAACTCCTCCCCCATCCT
CGCATGGAATCTCAATAGGACCAGGGGCCAGTTTTGCTAGTCCACATGGAACCCTTGACCCTAGTTCTCC
ATATACTATGGTGTCACCAAGTGGACGAGCAGGAAACTGGCCTGGGTCACCTCAAGTGTCCGGACCCTCA
CCAGCAACCCGTTTGCCTGGAATGTCACCAGCTAACCCATCTCTGCATTCTCCTGTTCCAGATGTTTCTC
ATTCTCCTCGAGCTGGCACAAGTTCCCAGACAATGCCAACCAACATGCCTCCACCTCGAAAACTACCTCA
GCGCTCCTGGGCAGCATCTATACCCACCATTCTCACTCATAGTGCCTTGAACATCTTACTGCTACCATCT
CCAACGCCAGGCCTTGTGCCTGGCCTGGCAGGTAGTTACCTTTGTTCCCCACTTGAGAGATTCCTTGGAT
CTGTCATCATGAGACGACACCTTCAAAGAATCATCCAACAGGAAACGTTGCAGCTGATCAATTCCAATGA
ACCTGGTGTAATCATGTTCAAGACAGATGCCCTGAAATGCAGAGTAGCCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAATCATCCACCCACATCAG; Reverse Primer - name:unspecified, sequence:GGGCTACTCTGCATTTCAGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4826151 same experiment
 EurExpress:euxassay_019541 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS