EMAGE mouse gene expression spatial database

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EMAGE:13713

Foxo3 forkhead box O3 ( MGI:1890081)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019517. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019517_01	euxassay_019517_02	euxassay_019517_03	euxassay_019517_04

euxassay_019517_05	euxassay_019517_06	euxassay_019517_07	euxassay_019517_08	euxassay_019517_09

euxassay_019517_10	euxassay_019517_11	euxassay_019517_12	euxassay_019517_13	euxassay_019517_14

euxassay_019517_15	euxassay_019517_16	euxassay_019517_17	euxassay_019517_18	euxassay_019517_19

euxassay_019517_20	euxassay_019517_21	euxassay_019517_22	euxassay_019517_23	euxassay_019517_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13713_wholemount_strong.wlz

13713_wholemount_moderate.wlz

13713_wholemount_weak.wlz

13713_wholemount_possible.wlz

13713_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13713_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 17 moderate expression: see section 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 09 moderate expression: see section 19 21 22 weak expression: see section 10 11 18 20

vibrissa

weak

regional

weak expression: see section 06 07 21 22 23

stomach

moderate

regional

moderate expression: see section 09 weak expression: see section 10 11

hindgut

weak

regional

weak expression: see section 15

midgut

moderate

regional

moderate expression: see section 09 17 18 19 weak expression: see section 10 11 12 13 14 15 16

liver

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 21 22 23 24 weak expression: see section 01 12 13 14 20

lung

strong

regional

strong expression: see section 09 10 11 13 17 18 19 21 22 23 24 moderate expression: see section 05 06 07 12 14 15 16 weak expression: see section 08 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55060
Entity Detected:	Foxo3, forkhead box O3 ( MGI:1890081)
Sequence:	sense strand is shown >T55060 CCTCATCTCCACACAGAACGTTGTTGGTTTGAATGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCT CAAAGCTGGGTACCAGGCTGAAGGATCACTGAGGAAAGGGGAAATGGGCAAAGCAGACCCTCAAACTGAC GGAAGACCTACAGAGAAAACCCTTTGCCAAATCTGCTCTCAGCAAGTGGACAGTGATCCGTTTACAGCTT GACACCTTTGAGACTCCCACGCCGCTTCCCTAACCCAGCAGAGACTGTTAGCAGCCCTGGCCCTGGGTGA AGCCCTTACCCGTGGAACAGAACTCTATAACATATGCAAAATCTAACTCATCTGCAAGTGACCTGCCAGC GTGGACAGCACCCATCAGCACCCCCCACTCCTTCAGAGCACACCGGGACCCCGTTGGCGACTCCGTCGTG TTTTACTATCATGATGGTTTAGGGGATATTTTAAGTGTCGTCTTGTGTTTGTTTCCTTTGACCCTCTGAG TTTTCTACACACAGTAACCTGCAGTATTTTTCTGTTGAAAATGTTAACTGTCCTTTCCCTAGCACACTTA AAAGCAGAAGGAAGGTGTTATATCAGTTCCCAGTCTGGCCTTGAGCATCGCAAGCTTTTGAG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCTCATCTCCACACAGAACG; Reverse Primer - name:unspecified, sequence:CTCAAAAGCTTGCGATGCTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824913 same experiment
	EurExpress:euxassay_019517 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source