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EMAGE:13715

Foxn2 forkhead box N2 ( MGI:1347478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715
"Pseudo-wholemount" of euxassay_019499. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019499_01 euxassay_019499_02 euxassay_019499_03 euxassay_019499_04
EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715
euxassay_019499_05 euxassay_019499_06 euxassay_019499_07 euxassay_019499_08 euxassay_019499_09
EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715
euxassay_019499_10 euxassay_019499_11 euxassay_019499_12 euxassay_019499_13 euxassay_019499_14
EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715
euxassay_019499_15 euxassay_019499_16 euxassay_019499_17 euxassay_019499_18 euxassay_019499_19
EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715 EMAGE:13715
euxassay_019499_20 euxassay_019499_21 euxassay_019499_22 euxassay_019499_23 euxassay_019499_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13715Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13715_wholemount_strong.wlz
13715_wholemount_moderate.wlz
13715_wholemount_weak.wlz
13715_wholemount_possible.wlz
13715_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13715_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 08 09 10 11 18 19 20 21
vibrissa
weak weak
regionalweak expression: see section 06 07 08 21 22
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 weak expression: see section 17
telencephalon ventricular layer
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 06 07 08 09 11 12 13 14 16 17 18 20 21 22 23
midbrain ventricular layer
weak weak
regionalweak expression: see section 14 15 17 18
mandible
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24
lower jaw incisor
weak weak
regionalweak expression: see section 12 13 16 17
lower jaw molar
weak weak
regionalweak expression: see section 07 19 20
maxilla
weak weak
regionalweak expression: see section 06 07 08 09 10 18 19 20 21 22
upper jaw incisor
weak weak
regionalweak expression: see section 12 13 16 17
upper jaw molar
weak weak
regionalweak expression: see section 07 19 20
liver
moderate moderate
regionalmoderate expression: see section 13 14 17 18 19 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 20 21 22 23 24
testis
weak weak
regionalweak expression: see section 09 10 11 22 23 24
urethra of male
weak weak
regionalweak expression: see section 16
lung
weak weak
regionalweak expression: see section 07 08 09 12 13 14 15 16 17 18 19 20 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55058
Entity Detected:Foxn2, forkhead box N2 ( MGI:1347478)
Sequence:sense strand is shown

>T55058
GGCTCAGGTTAGTTTCCTGCTTTCAAATGTTTTTATGACTATGGACTTTTTTAAAGAGACAAATGTTTTT
ATTTTTACTTCTAATTTCTTTCTGGGCAATACTGTCAAATGAGAAGAAAAACAGAAGCCCTGTTTCTTAA
TTGACACCACTTAAGCTATAACAGTATTGATATACTCCCGTTTCCATGTTAGTTGTAATTAAATCTGGAT
AGCCGACTTAATGGAATTGGTGCTACTAAAATATAGACATTTTTCTTTTATTGATAATGCAGGATGAAGA
ATTTGGAATTTTTCTAATTCATAAATGTCATCTTTCTAGAAAACAGTTAAATGAAGTTAGTTTTTACTGC
AGTGAATAAAACACCAAGCAGAAGTGTGAAGTGTGGTCTTATCGTGGTACAAGTCTTAAAGAGATGGTAG
AGTTCAGTGGATAGCAGGGAATTGTGAAGCAGGTCACCACGCCCTTCTGGGTGGACCTGTCACAGGGTCA
CCACATTCCGTGCTTCACCAGCAGTATGTCTCTCAAGGCCCTGTGCAGCGGGCATGTTGTTCTGCAAATG
GAGGAAAATGGTCTGAAGTAGGAGGAAAGAGTTGTTCTCAGCTCCAAGAGAAAGACCTCTCCAGAATTCG
TGTTTAGGCAGTAACTTAAATTCCTTTCCCTCCTCCTTCAAATGGCCTCTTACTTAAAGCAACAGCCATT
AGCAAGGATGATGCATGCCCGTGCACTCAAGACTAATAAGCAGTGTTCTCTAACACCTTGCACTTGGCTC
TCTTACAAGGATTCGATTTCTCACTTTTCATTTGCTGCTGTGTCTAAGCATCCTCCTTCAAGGGGAGAGA
GAGCCTTTGAGATCTGGTCCCTTGTGATGTTCTTAGAGACAGATTATGCTCCTGTTTGGTGTGCTCAGAT
TGAAGCCTGGCTTGGAACCATCCAGGCGACGAATTGTATGGGCTAGAAATTCCCAATAGATAACAAGCTT
TGTGTATGAAGTAAACACGGCTTAGAAATCCAGGGACAGCCCAGGTCTCAGTGAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GGCTCAGGTTAGTTTCCTGCT; Reverse Primer - name:unspecified, sequence:ATCACTGAGACCTGGGCTGT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13714 same embryo
 EurExpress:euxassay_019499 same experiment
 MGI:4824910 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS