EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13729

Fubp3 far upstream element (FUSE) binding protein 3 ( MGI:2443699)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019536. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019536_01	euxassay_019536_02	euxassay_019536_03	euxassay_019536_04

euxassay_019536_05	euxassay_019536_06	euxassay_019536_07	euxassay_019536_08	euxassay_019536_09

euxassay_019536_10	euxassay_019536_11	euxassay_019536_12	euxassay_019536_13	euxassay_019536_14

euxassay_019536_15	euxassay_019536_16	euxassay_019536_17	euxassay_019536_18	euxassay_019536_19

euxassay_019536_20	euxassay_019536_21	euxassay_019536_22	euxassay_019536_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13729_wholemount_strong.wlz

13729_wholemount_moderate.wlz

13729_wholemount_weak.wlz

13729_wholemount_possible.wlz

13729_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13729_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 11 19 20 21

vibrissa

moderate

regional

moderate expression: see section 06 07 09 19 20 21 weak expression: see section 08

ventral grey horn

weak

regional

weak expression: see section 16 19 20

dorsal root ganglion

weak

regional

weak expression: see section 11 13 14 15 20 21

lower jaw incisor

moderate

regional

moderate expression: see section 16 17 weak expression: see section 11 12 13

lower jaw molar

weak

regional

weak expression: see section 09 19

upper jaw incisor

moderate

regional

moderate expression: see section 16 weak expression: see section 11 12 13

upper jaw molar

weak

regional

weak expression: see section 09 19

metanephros

weak

regional

weak expression: see section 10 11 12 13 14 21 22 23

lung

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55062
Entity Detected:	Fubp3, far upstream element (FUSE) binding protein 3 ( MGI:2443699)
Sequence:	sense strand is shown >T55062 TCTCCTCTGTCCCTCCACTCCATAACTGAGCTTGAGCAGAGGCTGCTTCTAGCTCTGGGCCTAGAAACCC TGACTATACCTAAGTGCCCTGGGTCTGCAGTGAGCCAGGACCACTCCAGCAGTAGTTCTTGCAGAGAGTG GCCAGGTTGGGCCTTTACCTGCACAGCACCTTTGGGGCCAAGCAAGTGGAGGGCCAGCTTGGCCTCTTTC TGTGTAGAAATGAGGAGGGCTCTCTCCCGTTCTCAGCCAAGAAGGAGCTCTCTTTCCTTGGACCTTGGGG GCAAGGCTCCCTCCCAGGGGGTACCTTTTGCCTTGTAGGAAGCTGGGGTTAGACCTGGTGACCCAGGAGA AGCCTTCCCCTTTCACCCCATACCCCCACATTCTGACCCTAGAACCTGAGGTGCTTAGCATTCAGTACCT GGTGGTATGAGACTCTGGTCTTGGGCTGACCCTCTCCCTCCCCCCACAGGAGCAGTAGATGGGCATCTGC CGCGGCTCTTCCCCGAAATCAGTGTGAGAGAAACCTGTTTGTTACAGAGATTTTTAGATTTGCAAACCTT TTGATGAAGACCTTTGTTTTGTTCTGTGAAACACTATATTTAGATTAACAGAAATTTTCTAAAAATTGGG AAAATTAGTTACTAAACATTGCTGGTAATTTTTACGTCATGATTTCTTTGTAAATGTGTGAGCTCCGGGG CTCTTGGAGCGAAACCTGCAAGCTCGGGTGCCCAAGCTCTCCTCGGAGCCTGGCATTTCATCACACGATG GCTTTGTCTTGTGTCTTTTTAGTTACAGTTTGGGGTGGGTTTG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCTCCTCTGTCCCTCCACTC; Reverse Primer - name:unspecified, sequence:CAAACCCACCCCAAACTGTA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13730 same embryo
	EMAGE:13728 same embryo
	EurExpress:euxassay_019536 same experiment
	MGI:4824942 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source