EMAGE mouse gene expression spatial database

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EMAGE:13730

Bdp1 B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB ( MGI:1347077)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019513. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019513_01	euxassay_019513_02	euxassay_019513_03	euxassay_019513_04

euxassay_019513_05	euxassay_019513_06	euxassay_019513_07	euxassay_019513_08	euxassay_019513_09

euxassay_019513_10	euxassay_019513_11	euxassay_019513_12	euxassay_019513_13	euxassay_019513_14

euxassay_019513_15	euxassay_019513_16	euxassay_019513_17	euxassay_019513_18	euxassay_019513_19

euxassay_019513_20	euxassay_019513_21	euxassay_019513_22	euxassay_019513_23	euxassay_019513_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13730_wholemount_strong.wlz

13730_wholemount_moderate.wlz

13730_wholemount_weak.wlz

13730_wholemount_possible.wlz

13730_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13730_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 weak expression: see section 08 10 11 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 06 21 22 weak expression: see section 05 07 08 20

dorsal root ganglion

weak

regional

weak expression: see section 12 14 19 20 21

midgut

moderate

regional

moderate expression: see section 06 07 17 18 weak expression: see section 08 09 10 11 12 14 15 16 19

lower jaw incisor

weak

regional

weak expression: see section 11 12 13 16 17

upper jaw incisor

weak

regional

weak expression: see section 11 12 15 16 17

lung

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23

nucleus pulposus

weak

regional

weak expression: see section 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55012
Entity Detected:	Bdp1, B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB ( MGI:1347077)
Sequence:	sense strand is shown >T55012 CAAGAACAGCAGCCACTTCAAGTTAAACCAGCTCCCTTTCTGAGAAGTCGATTCAAAAAACCAAAACCAA ACCTGTCAAGAGCAGCTTTAAAGAGAGCAACCATAGAAGCAGAGCACTGTGTGCCTGGGAAGAAATCCGA AGCCTGCAAAGTGGAGGCCGCCATGCTACAGCAGGACAGTGACCAAGCTGCTCTCTCTCCTCAACATAAT GTACCTTCCCTGATGGCATCAAGAGAAAATGATAAATCCGGTCATGAGGAGGAGGAGGAGGCTGCGATAC TACCATGTACACAGACTGAAAAGGATGCTTCACCGCCAAATTCAAGTGAACCTAAAGAGGGTTCTCAGTT AACCCCAAACCAGGAAAATGGCTTACTTGTTCCTATTGGGACTCCCATGATGAATACGGTCACACAAGAA ACAAGGCAAAATGTTGTCCAGACAACTCTCCCAGTGAGGGGTCGACTTCAGAGACCAAGACCAAATGTGC AAAAGGCCAGACAGAGGCAAATAGTAGAAAAAGGCGAAGCGCGAGACATAGCTAAGAATGAAGGACCAGA ACTGCAAAAAGACGAGACTAGAACATGCCTGACTGTGGCAAACTCTTCTCACATTGAAAGTGGAGTTGCA GTTGACATGTCCTCCAGAGTGTCAGAGTGCCAAGTGAGTGAAAGTCAAGGCCACGCAGATCCTGTAGAAA ACTTAAGTGTTAATAAAGCCAGTGTTGTTCATGAACAGATGAGGCATGAAAATAAACCGTATGTTCCTAG TCCAGCACTGCTGATAAGAAGGCGGTTCCAAAAGGCTAAACCAAATTTAGGAGGAGCACGCCGTAAGGAT GAGCAACCAGGTGTGGAGAAAGGCCGGACAGACGAGAGCACAGCACTAACAGCAGAAGATCACCTGCTGC AGAAGGAAGACTGTGACACCCAGCTTTCTCTGCAAGCAAGGGAAAAGGCAGATATGCCTCTGGAGGTTTC AGTGAGAAAAGAGTGTATACATTCTGAAGAGTCGGGTTCTGACAGAAACGATGCTCAGCCGAACGCTGGA CCATCAGAAGGTTCCAGGGATGAGACTGCGAAAGAGC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAAGAACAGCAGCCACTTCA; Reverse Primer - name:unspecified, sequence:GCTCTTTCGCAGTCTCATCC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13728 same embryo
	EMAGE:13729 same embryo
	EurExpress:euxassay_019513 same experiment
	MGI:4823457 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source