EMAGE mouse gene expression spatial database

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EMAGE:13742

Zhx3 zinc fingers and homeoboxes 3 ( MGI:2444772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019571. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019571_01	euxassay_019571_02	euxassay_019571_03	euxassay_019571_04

euxassay_019571_05	euxassay_019571_06	euxassay_019571_07	euxassay_019571_08	euxassay_019571_09

euxassay_019571_10	euxassay_019571_11	euxassay_019571_12	euxassay_019571_13	euxassay_019571_14

euxassay_019571_15	euxassay_019571_16	euxassay_019571_17	euxassay_019571_18	euxassay_019571_19

euxassay_019571_20	euxassay_019571_21	euxassay_019571_22	euxassay_019571_23	euxassay_019571_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13742_wholemount_strong.wlz

13742_wholemount_moderate.wlz

13742_wholemount_weak.wlz

13742_wholemount_possible.wlz

13742_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13742_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 08 09 10 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 07 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 10 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 11 20

cervico-thoracic ganglion

weak

regional

weak expression: see section 13 19

thoracic ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 13 14 18 19 21 weak expression: see section 12 20

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 17

metanephros

moderate

regional

moderate expression: see section 10 11 23 weak expression: see section 12 13 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55229
Entity Detected:	Zhx3, zinc fingers and homeoboxes 3 ( MGI:2444772)
Sequence:	sense strand is shown >T55229 TGAGCCTGAGGAAGATGGTTTGAACAAGTTGGTAGAACAGCCACCAAGCAAAGTAAGCTACAAAAAGACC GCACAGCAGCGTCATTTGCTTCGGCAGCTCTTTGTCCAAACACAGTGGCCAAGCAACCAGGACTATGACT CCATTATGGCCCAAACAGGGCTGCCCCGGCCAGAGGTGGTACGCTGGTTTGGAGATAGCAGGTATGCCCT TAAGAATGGCCAGCTCAAGTGGTACGAAGACTATAAGAGGGGAAACTTCCCACCAGGTTTGCTGGTCATA GCCCCTGGCAACCGAGAGCTGCTGCAAGATTATTACATGACACACAAGATGTTGTGTGAGGAAGATCTGC AGACCCTCTGTGATAAGACCCAGATGAGTGCCCAGCAGGTCAAGCAGTGGTTTGCTGAGAAGATGGGTGA AGAGACCCGGGCTGTGGCAGATATAAGCAGTGAGGATCAGGGCCCTAGAAATGGAGAGCCTGTGGCAGTT CACAAGGTGCTGGGTGATGCCTATTCGGAGCTGTCTGAGAACAGCGAATCATGGGAGCCAAGTGCTCCTG AGGCCAGCTCAGAGCCTTTTGACACTTCAAGTCCCCAGTCTGGACGTCAACTCGAAGCAGACTGAATTTG ATCTTGATTACTGTGAAGGACCGGCCAGTCTGGGATGCTGTATGCCATGTGGAAGGGCCAAAACTGCCAC ATATCATCTTGATGGCCAGCTGCCAGGCACCAGGAGAGCCTCTGGAGCCTCGCTGAACCCAGAACCTGCC ACCGCCCCAACCACCTTCCGCCTGACATTTCTGAGCAAATAGCAATGGTTTCTCATCAGCCCATCTTCCC ACAGAGCAGGACCTTTCCATGGCCTCCCAGGGACAATTCAGATTGCATATACCTAGACCCAGAATCAAGT TGTTAGCATATACAGCTGCCTATATTCATTTAATAAAGCATACTACTACAGACATTTTCAATACATAGGA ACTCGGGTTACCAAAACAGTACAGCTCTGACAATGCCATGTCTGTTTTCTGGGAAGCTGAGACCACAGGG AC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGAGCCTGAGGAAGATGGTT; Reverse Primer - name:unspecified, sequence:GTCCCTGTGGTCTCAGCTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13741 same embryo
	EMAGE:13743 same embryo
	EMAGE:13740 same embryo
	EurExpress:euxassay_019571 same experiment
	MGI:4829352 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source