EMAGE mouse gene expression spatial database

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EMAGE:13747

Mir183 microRNA 183 ( MGI:2676847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019222. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019222_01	euxassay_019222_02	euxassay_019222_03	euxassay_019222_04

euxassay_019222_05	euxassay_019222_06	euxassay_019222_07	euxassay_019222_08	euxassay_019222_09

euxassay_019222_10	euxassay_019222_11	euxassay_019222_12	euxassay_019222_13	euxassay_019222_14

euxassay_019222_15	euxassay_019222_16	euxassay_019222_17	euxassay_019222_18	euxassay_019222_19

euxassay_019222_20	euxassay_019222_21	euxassay_019222_22	euxassay_019222_23	euxassay_019222_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13747_wholemount_strong.wlz

13747_wholemount_moderate.wlz

13747_wholemount_weak.wlz

13747_wholemount_possible.wlz

13747_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13747_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 03 04 05 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17 18

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 16 17 18

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70295
Entity Detected:	Mir183, microRNA 183 ( MGI:2676847)
Sequence:	sense strand is shown >T70295 TATGGCACTGGTAGAATTCACT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-183 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13749 same embryo
	EMAGE:13746 same embryo
	EMAGE:13750 same embryo
	EMAGE:13745 same embryo
	EMAGE:13748 same embryo
	EurExpress:euxassay_019222 same experiment
	MGI:4826227 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source