Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13755

Gtf2h2 general transcription factor II H, polypeptide 2 ( MGI:1345669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755
"Pseudo-wholemount" of euxassay_019537. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019537_01 euxassay_019537_02 euxassay_019537_03 euxassay_019537_04
EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755
euxassay_019537_05 euxassay_019537_06 euxassay_019537_07 euxassay_019537_08 euxassay_019537_09
EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755
euxassay_019537_10 euxassay_019537_11 euxassay_019537_12 euxassay_019537_13 euxassay_019537_14
EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755
euxassay_019537_15 euxassay_019537_16 euxassay_019537_17 euxassay_019537_18 euxassay_019537_19
EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755 EMAGE:13755
euxassay_019537_20 euxassay_019537_21 euxassay_019537_22 euxassay_019537_23 euxassay_019537_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13755Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13755_wholemount_strong.wlz
13755_wholemount_moderate.wlz
13755_wholemount_weak.wlz
13755_wholemount_possible.wlz
13755_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13755_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 12 13 14 15
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18 19
pancreas
weak weak
regionalweak expression: see section 10 11 12 13 18 19
vibrissa
weak weak
regionalweak expression: see section 05 06 07 20 21 22
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 17 18 19 21 weak expression: see section 06 16 20
lower jaw incisor
weak weak
regionalweak expression: see section 10 11 12 15 16
upper jaw incisor
weak weak
regionalweak expression: see section 11 12 15 16
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 09 10 11 12 13 14 15 17 18 19 21 22 23 weak expression: see section 04 08 20 24
metanephros
weak weak
regionalweak expression: see section 09 10 11 12 13 19 20 21 22 23
lung
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55068
Entity Detected:Gtf2h2, general transcription factor II H, polypeptide 2 ( MGI:1345669)
Sequence:sense strand is shown

>T55068
AGCTGAGGACCGAGAGTTGAATAAGCATATATATCATTATAAGCACCATGGATGAAGAACCTGAGAGAAC
CAAGCGGTGGGAAGGAGGCTATGAGAGAACCTGGGAAATTCTTAAAGAAGATGAAACTGGATCACTTAAA
GCTACAATAGAAGATATTCTCTTCAAGGCAAAGAGGAAAAGAGTGTTTGAGCACCATGGACAAGTCCGAC
TTGGAATGATGCGCCACCTGTATGTGGTGGTGGATGGATCGAGAACAATGGAAGATCAGGATTTAAAGCC
CAATAGACTGACTTGCACTTTAAAGTTGCTGGAATACTTTGTAGAAGAATATTTTGATCAAAACCCTATC
AGTCAGATTGGAATAATTGTAACTAAGAGTAAAAGAGCTGAAAAACTGACTGAACTCTCAGGAAACCCAA
GGAAACATATAACATCTTTGAAGAAAGCTGTAGATATGACCTGCCATGGAGAACCATCGCTCTATAATTC
CTTAAGCATGGCTATGCAGACCCTAAAACACATGCCTGGACATACAAGTAGAGAAGTGCTCATCATCTTC
AGTAGCCTCACCACCTGTGATCCATCTAATATTTACGATCTCATCAAGACCCTGAAGACAGCTAAAATTA
GAGTGTCTGTTATTGGATTATCTGCGGAGGTTCGAGTTTGTACTGTACTTGCTCGTGAAACTGGTGGCAC
ATACCATGTTATCTTAGATGAAACCCATTACAAGGAGTTGTTGGCACATCATGTGAGCCCCCTTCCTGCC
AGCTCAAGCTCCGAGTGCTCACTCATTCGCATGGGATTCCCTCAGCATACCATTGCTTCTTTGTCTGATC
AGGATGCAAAACCATCCTTCAGCATGGCGCATTTGGATAACAACAGCACTGAGCCAGGGCTTACACTGGG
AGGCTACTTCTGC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGCTGAGGACCGAGAGTTGA; Reverse Primer - name:unspecified, sequence:GCAGAAGTAGCCTCCCAGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13754 same embryo
 EMAGE:13753 same embryo
 EMAGE:13752 same embryo
 EurExpress:euxassay_019537 same experiment
 MGI:4825274 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS