EMAGE mouse gene expression spatial database

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EMAGE:13758

Hmgb2 high mobility group box 2 ( MGI:96157)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_019251_01	euxassay_019251_02	euxassay_019251_03	euxassay_019251_04	euxassay_019251_05

euxassay_019251_06	euxassay_019251_07	euxassay_019251_08	euxassay_019251_09	euxassay_019251_10

euxassay_019251_11	euxassay_019251_12	euxassay_019251_13	euxassay_019251_14	euxassay_019251_15

euxassay_019251_16	euxassay_019251_17	euxassay_019251_18	euxassay_019251_19	euxassay_019251_20

euxassay_019251_21	euxassay_019251_22	euxassay_019251_23	euxassay_019251_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13758_wholemount_strong.wlz

13758_wholemount_moderate.wlz

13758_wholemount_weak.wlz

13758_wholemount_possible.wlz

13758_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13758_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 12 13 17 18 19 20 21 22 23 24

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 09 10 11 12 15

thyroid gland

strong

regional

strong expression: see section 10 11 15 16

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

rest of cerebellum marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

spinal cord ventricular layer

strong

regional

strong expression: see section 15 16 moderate expression: see section 13 14 weak expression: see section 12

neural retina

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 04 22 23 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

heart valve

moderate

regional

moderate expression: see section 13 14 weak expression: see section 11

heart ventricle

moderate

regional

moderate expression: see section 08 09 10 13 14 15 16 17 weak expression: see section 11 12

esophagus

moderate

regional

moderate expression: see section 12 13

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

lower jaw molar

moderate

regional

moderate expression: see section 07 19 20

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16 17

upper jaw molar

moderate

regional

moderate expression: see section 07 19 20

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metanephros

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19 20 21

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trachea

moderate

regional

moderate expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T114
Entity Detected:	Hmgb2, high mobility group box 2 ( MGI:96157)
Sequence:	sense strand is shown >T114 AAGAAGCATCCCGACTCGTCGGTGAACTTCGCCGAGTTCTCCAAGAAATGCTCCGAGAGATGGAAGACCA TGTCTGCAAAGGAAAAGTCCAAGTTTGAAGATTTGGCCAAGAGCGACAAAGCTCGTTATGACAGGGAGAT GAAGAACTATGTTCCTCCCAATAGGGGATAAGAAAGGAAAGAAAAAAGACCCCAATGCTCCGAAGAGACC ACCGTCTGCCTTCTTCCTGTTTTGCTCTGAAAATCGCCCAAAGATCAAAATTGAACACCCAGGCCTGTCT ATTGGAGATACTGCGAAGAAACTGGGTGAGATGTGGTCTGAGCAATCTGCCAAAGATAAACAACCGTATG AGCAGAAAGCAGCTAAACTAAAGGAGAAGTATGAAAAGGATATTGCTGCATACCGTGCCAAGGGCAAAAG TGAAGCAGGAAAGAAGGGTCCTGGTAGGCCAACAGGCTCAAAGAAGAAGAACGAACCAGAAGATGAGGAG GAGGAAGAAGAGGAGGAAGAGGAGGAAGATGACGAGGAAGAAGAG
Notes:	The probe template was PCR amplified from IMAGE:614347 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:614347 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13756 same embryo
	EMAGE:13757 same embryo
	EurExpress:euxassay_019251 same experiment
	MGI:4825391 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source