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EMAGE:1376

Rarb retinoic acid receptor, beta ( MGI:97857)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1376
0098b.jpg This embryo was treated with ProteinaseK for 30min.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1376Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1376_wholemount_strong_3D_1.wlz
1376_wholemount_moderate_3D_1.wlz
1376_wholemount_weak_3D_1.wlz
1376_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1376_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
Expression was detected in dorsal and ventral rhombencephalon.
future spinal cord
detected detected
Expression was detected in dorsal and ventral spinal cord.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3505986
Entity Detected:Rarb, retinoic acid receptor, beta ( MGI:97857)
Sequence:sense strand is shown

>MGI:3505986
CAGTGAGCTGGCCACCAAGTGCATTATTAAGATCGTGGAGTTCGCCAAGCGTCTGCCGGGCTTCACAGGT
CTGACCATCGCAGACCAGATCACCCTGCTCAAAGCCGCCTGCTTGGATATCTTGATTCTCAGAATTTGTA
CCAGGTATACCCCAGAGCAAGACACCATGACTTTCTCTGATGGCCTTACACTAAATCGAACTCAGATGCA
CAATGCTGGCTTCGGTCCTCTGACTGACCTTGTGTTCACCTTTGCCAACCAGCTCCTGCCTTTGGAAATG
GATGACACAGAAACAGGCCTTCTCAGTGCCATCTGTTTAATCTGTGGAGACCGCCAGGACCTTGAGGAAC
CAACAAAAGTAGACAAGCTCCAAGAACCACTGCTGGAAGCACTAAAGATTTACATTAGAAAACGACGACC
CAGCAAGCCTCACATGTTTCCAAAGATCTTAATGAAAATCACAGATCTCCGCAGCATCAGCGCGAAAGGT
GCCGAACGTGTAATTACCTTGAAAATGGAAATTCCTGGATCAATGCCACCTCTCATTCAGGAAATGCTGG
AGAATTCTGAAGGACATGAACCCTTGACCCCAAGTTCAAGTGGGAATATAGCAGAGCACAGTCCCAGCGT
GTCCCCCAGCTCAGTGGAGAACAGTGGAGTCAGTCAGTCACCACTGCTGCAGTGAGACATTTCCAGCTGT
TGCAGACATTCCC
nt 837 - nt 1549 of XM_127583.3
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: cgcggatcccagtgagctggccaccaagtgc 3'primer: cccaagcttgggaatgtctgcaacagctgg Editors Note: Original information from the authors states that these two primers were used to generate a 712nt fragment (nt1021-1733 in XM_127583). Pairwise comparisons of these two primer sequences against the 3 public versions of XM_126744 only identifies a 712nt fragment in XM_127583.3 which extends from nt837-1549. This is therefore indicated above as the nucleotide sequence of the probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508703 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE