EMAGE mouse gene expression spatial database

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EMAGE:13774

Mir199a-2 microRNA 199a-2 ( MGI:3618742)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019241. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019241_01	euxassay_019241_02	euxassay_019241_03	euxassay_019241_04

euxassay_019241_05	euxassay_019241_06	euxassay_019241_07	euxassay_019241_08	euxassay_019241_09

euxassay_019241_10	euxassay_019241_11	euxassay_019241_12	euxassay_019241_13	euxassay_019241_14

euxassay_019241_15	euxassay_019241_16	euxassay_019241_17	euxassay_019241_18	euxassay_019241_19

euxassay_019241_20	euxassay_019241_21	euxassay_019241_22	euxassay_019241_23	euxassay_019241_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13774_wholemount_strong.wlz

13774_wholemount_moderate.wlz

13774_wholemount_weak.wlz

13774_wholemount_possible.wlz

13774_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13774_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 24

exoccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 23

temporal bone

moderate

regional

moderate expression: see section 01 02 03 21 22 23

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

tail mesenchyme

moderate

regional

moderate expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70315
Entity Detected:	Mir199a-2, microRNA 199a-2 ( MGI:3618742)
Sequence:	sense strand is shown >T70315 CCCAGTGTTCAGACTACCTGTTC
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-199a-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13775 same assay
	EurExpress:euxassay_019241 same experiment
	MGI:4826234 same experiment
	MGI:4826235 same experiment
	EMAGE:13776 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source