EMAGE mouse gene expression spatial database

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EMAGE:13782

Prdm15 PR domain containing 15 ( MGI:1930121)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019611_01	euxassay_019611_02	euxassay_019611_03	euxassay_019611_04

euxassay_019611_05	euxassay_019611_06	euxassay_019611_07	euxassay_019611_08	euxassay_019611_09

euxassay_019611_10	euxassay_019611_11	euxassay_019611_12	euxassay_019611_13	euxassay_019611_14

euxassay_019611_15	euxassay_019611_16	euxassay_019611_17	euxassay_019611_18	euxassay_019611_19

euxassay_019611_20	euxassay_019611_21	euxassay_019611_22	euxassay_019611_23	euxassay_019611_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13782_wholemount_strong.wlz

13782_wholemount_moderate.wlz

13782_wholemount_weak.wlz

13782_wholemount_possible.wlz

13782_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13782_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 21 22 weak expression: see section 05 06 07 08

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 19

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18 19

neural retina

weak

regional

weak expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 15 weak expression: see section 09 10 12 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 11 weak expression: see section 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55162
Entity Detected:	Prdm15, PR domain containing 15 ( MGI:1930121)
Sequence:	sense strand is shown >T55162 GGACATGCTGAAAGAGCACATGCGTGTGCATGACAACATCCGGGAGTACCTGTGCGCCGAGTGTGGGAAA GGTATGAAGACCAAGCATGCACTGCGCCACCATATGAAGCTGCACAAAGGCATCAAGGAGTATGAGTGCA AGGAATGTCACCGCAAGTTTGCACAGAAGGTCAACATGCTCAAGCACTACAAACGGCACACAGGAATTAA AGACTTCATGTGTGAATTGTGTGGGAAGACATTCAGTGAGAGGAACACAATGGAGACACACAAGCTCATC CACACAGTAGGCAAGCAGTGGACTTGTTCCGTGTGTGACAAGAAGTACGTCACAGAGTACATGCTTCAGA AACACGTCCAGCTCACTCATGACAAGGTGGAAGCACAGAGTTGCCAACTATGTGGCACCAAAGTGTCAAC CCGGGCCTCCATGAGCAGACATATGCGACGCAAGCACCCCGAGGTCCTCGCTGTTCGGATTGACGATCTG GACCACCTCCCCGAGACCACCACCATCGATGCATCTTCCATCGGCATTGTCCAGCCTGCGCTGGGCCTGG AGCAGGAAGAACTGGCAGAAGGTAAGCATGGAAAGGCTGCCAAGCGCAGCCACAAGAGAAAGCAGAAGCC AGAGGAAGAGGCAGGGGCTCCAGTGCCTGAGGATACCACCTTCAGTGAATACCCCGAAAAGGAGCCAGAG TTCACAGGCAGTGTGGGTGACGAGACAAATTCTGCTGTACAGAGTATCCAACAGGTGGTGGTGACCCTAG GGGACCCCAATGTGACTGCTCCATCAAGCTCAGTTGGTCTAACCAACATCACCGTGACCCCCATCACCAC TGCAGCTGGAACTCAGTTTACCAATTTACAGCCAGTGGCTGTTGGACATCTTACCAACCCTGATCGCCAG CTACAGCTAGATAACTCCATCCTGACTGTGACCTTTGATACTGTCAGTGGCTCCGCCATGTTGCACAACC GGCAAAATGATGTCCAGATCCACCCACAGCCAGAAGCCACAAACCCTCAGTCAGTGGCCCACTTTATCAA CCTTACCACACTGGTAAACTCCATCACACCCTTGGGGAACCAACTCAGTGAGCAGCACCCACTCACCTGG CGAGCAGTGCCCCAGACAGATGTGCTGCAGCCGCCACAGGCCCCTGCAGCCCCGCAGCAGGCGGTGCAAC CCCAGGTACAGAATGAGCAGCAGCAGATGTACAGCTACTGATCTGTGGCTGCAGAATAGGCTGAGCTGCA GCAGTGGGGTTCTGAGGTCTTGAGGTGTTTGCTGGATACCAAACAGGAAGAGTTTGCAAATGTCAAGATC ACTGAGATTAGCATTACAGAGCTACCTATCAGCTTTGCTGGTGGTATGGCTTTGGCAAGCAGGAGCTTCT GCCATGTGTTTTCCCAAGGTGTTGCAGCCTGGCCAGACCACAGTGTCACCCTCAGAGGTCTGTAATTGTC ATGGGGCTCAGGGATACAGATAACAGGATCCTAGAAGCAGGCGTGATATTCCAAGGACCAGGGAGATTTG ATCTGTTTTCTTTAGCACTCCAGTGACAATAACTGAAATATGGCTAGCCTTCTGTGGCAACACACAGAAG CAAACGCAGGTTTCTCCTTGCTCCTGGATTCACAAGCAGGGAGAAGTCATTCTGAAGTCTCAGTTCTTAC GTCACAGAGCTGTGGCCTGAGGCCTCCTGTGAGAAGTATCATGAGGCCCCCAGCTCAGGCACAGAAGACA TCTTGGGAACATCTATAGACTGATTTTTATGAAGAGTCTGTTTTATTTCATAACCACGTTATGTATTATG GCCCTCTCGTGGTCAATATTGATTTCTTTTAATATGATAAAGTCGTTTGCACTGTAGGGTGTCAGTCCCT GTCCAGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GGACATGCTGAAAGAGCACA; Reverse Primer - name:unspecified, sequence:ACTGGACAGGGACTGACACC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13783 same embryo
	EMAGE:13786 same embryo
	EMAGE:13785 same embryo
	EMAGE:13784 same embryo
	EurExpress:euxassay_019611 same experiment
	MGI:4827399 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source