EMAGE mouse gene expression spatial database

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EMAGE:13790

Tlx1 T-cell leukemia, homeobox 1 ( MGI:98769)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019585. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019585_01	euxassay_019585_02	euxassay_019585_03	euxassay_019585_04

euxassay_019585_05	euxassay_019585_06	euxassay_019585_07	euxassay_019585_08	euxassay_019585_09

euxassay_019585_10	euxassay_019585_11	euxassay_019585_12	euxassay_019585_13	euxassay_019585_14

euxassay_019585_15	euxassay_019585_16	euxassay_019585_17	euxassay_019585_18	euxassay_019585_19

euxassay_019585_20	euxassay_019585_21	euxassay_019585_22	euxassay_019585_23	euxassay_019585_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13790_wholemount_strong.wlz

13790_wholemount_moderate.wlz

13790_wholemount_weak.wlz

13790_wholemount_possible.wlz

13790_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13790_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

spleen primordium

strong

regional

strong expression: see section 02 03 04 05 06 07

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 17 weak expression: see section 07 08 16

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 09 17 18 moderate expression: see section 10 16

pons mantle layer

strong

regional

strong expression: see section 07 08 17 moderate expression: see section 16

facial vii ganglion

strong

regional

strong expression: see section 05 21 moderate expression: see section 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 18 19

ventral grey horn

weak

regional

weak expression: see section 11 12 15 16

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

tongue

weak

regional

weak expression: see section 10 11 12 13 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 15 weak expression: see section 10 11 14 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55203
Entity Detected:	Tlx1, T-cell leukemia, homeobox 1 ( MGI:98769)
Sequence:	sense strand is shown >T55203 ACGACTCCACCAAAATCACCAGCGTCACGTCCGTGGCTTCGGCCTGCGAGTGAGGACCCAAGGCCCGTTG AGGACTTTCCGGAGAACCAGAACTCTCGACACCCTTTCTGACTGCACGCAGGAGGGAAATGGGGGGCTTC TCAGCAAGGCTCCCAAGCACCGCCTCCACTCCCCAGCGGACACTTCCTGTCTTCGGTGGAAGAGGGCTGG GGATACAGGCAGAGACATCTTCCCAGAAGCCTGTGTGATCCTGCCCTCACTCTGGAACAGTTCAGAATCC TCTGTTTTCCTCTATTTCATAAAATTTACTGATTTTTAACATGGGACAGAGAGACCCAAGGGAAGTAGGG TCCGGAAGGCTTCTGGGATCCCCAGGCAGCCATCTGTACTAAAGCTGGAAACCTCTCTGTTCCTCTCCTC GGAGGAGAGCCCGGAGGTCCACACAGAGGTGATACCACTGTCCCTCCTGGTGTCACCCAGAGCTACACAC AGGGGCCTATGGCAGAGCATCAATGCACACACAGGATCACAGCAAATGACCCTTGTTGTAGGGCATAGTC TGGGGTGACTCTCAGACTCACCCAAACAGCACGGACCTCAAACACACGGCCATAGTCACACTGTGACACA CACAACAGCTAAACTTGGCCTGTCAGGCCCTCAGCCACACATCCCAGCATCACCCAGGTCACCCAGGTCA CCCAGGTATGCACAGACAGGCTTTCACATAAATGCAGCCCATTTCTCCAGATCCTGTTTGGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACGACTCCACCAAAATCACC; Reverse Primer - name:unspecified, sequence:CCCAAACAGGATCTGGAGAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13789 same embryo
	EMAGE:13788 same embryo
	EMAGE:13793 same embryo
	EMAGE:13792 same embryo
	EMAGE:13791 same embryo
	EurExpress:euxassay_019585 same experiment
	MGI:4828744 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source