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EMAGE:13803

Ifi205 interferon activated gene 205 ( MGI:101847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13803 EMAGE:13803 EMAGE:13803 EMAGE:13803 EMAGE:13803
"Pseudo-wholemount" of euxassay_019604. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019604_01 euxassay_019604_02 euxassay_019604_03 euxassay_019604_04
EMAGE:13803 EMAGE:13803 EMAGE:13803 EMAGE:13803 EMAGE:13803
euxassay_019604_05 euxassay_019604_06 euxassay_019604_07 euxassay_019604_08 euxassay_019604_09
EMAGE:13803 EMAGE:13803 EMAGE:13803 EMAGE:13803 EMAGE:13803
euxassay_019604_10 euxassay_019604_11 euxassay_019604_12 euxassay_019604_13 euxassay_019604_14
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euxassay_019604_15 euxassay_019604_16 euxassay_019604_17 euxassay_019604_18 euxassay_019604_19
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euxassay_019604_20 euxassay_019604_21 euxassay_019604_22 euxassay_019604_23 euxassay_019604_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13803Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13803_wholemount_strong.wlz
13803_wholemount_moderate.wlz
13803_wholemount_weak.wlz
13803_wholemount_possible.wlz
13803_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13803_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
adrenal gland
weak weak
regionalweak expression: see section 13 14 21 22 23
thymus primordium
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 14 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55111
Entity Detected:Ifi205, interferon activated gene 205 ( MGI:101847)
Sequence:sense strand is shown

>T55111
TGAAAACACAGAACACAAGCTATGAAATAAAAGATGGTTCAGGAAGCATAGAAGTTGTGGGGAGTGGACA
ATGGCACAACATCAACTGTAAGGAAGGAGATAAGCTCCACCTCTTCTGCTTTCACCTGAAAAGAGAAAGA
GGACAACCAAAGTTAGTGTGTGGAGACCACAGTTTCGTCAAGGTCACCAAGGCTGGGAAAAAAAAAGAAG
CATCAACTGTCCAGTGAAGCACAAAAAATGAAGAAGAAAATGATTACCCAAAAGTTGGAATTAAGGTAGA
GATGCCAAAATAGAAATGTCACCTCTAAATGACAGCTTTAGTAGTATATCCAACCATTGATTAATCTTCA
TACCTGATTTCTGATTTTGTGTTTTCATTTGAAAAAATTCTTATTGTTCTGTTTTTCTATGAAAATAAAA
TTTGATTTCATTTCTCTACTGTAAAAATAATAAACATGTCTTTTTAAAGG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGAAAACACAGAACACAAGC; Reverse Primer - name:unspecified, sequence:CCTTTAAAAAGACATGTTTA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13804 same assay
 EMAGE:13805 same embryo
 EMAGE:13807 same embryo
 EurExpress:euxassay_019604 same experiment
 MGI:4825511 same experiment
 MGI:4825512 same experiment
 EMAGE:13806 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS