EMAGE mouse gene expression spatial database

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EMAGE:13805

Hoxb4 homeobox B4 ( MGI:96185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019595. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019595_01	euxassay_019595_02	euxassay_019595_03	euxassay_019595_04

euxassay_019595_05	euxassay_019595_06	euxassay_019595_07	euxassay_019595_08	euxassay_019595_09

euxassay_019595_10	euxassay_019595_11	euxassay_019595_12	euxassay_019595_13	euxassay_019595_14

euxassay_019595_15	euxassay_019595_16	euxassay_019595_17	euxassay_019595_18	euxassay_019595_19

euxassay_019595_20	euxassay_019595_21	euxassay_019595_22	euxassay_019595_23	euxassay_019595_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13805_wholemount_strong.wlz

13805_wholemount_moderate.wlz

13805_wholemount_weak.wlz

13805_wholemount_possible.wlz

13805_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13805_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 13 15 17 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 13 15 17 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 10 21

spinal cord mantle layer

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

metanephros

strong

regional

strong expression: see section 11 14 15 21 22 23 moderate expression: see section 10 12 13 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55077
Entity Detected:	Hoxb4, homeobox B4 ( MGI:96185)
Sequence:	sense strand is shown >T55077 AATAAGGGAGCCGGGTAAAGTCCTTCGGAGACAAGATTCGAGTCTCTTGCTTTCTTCCTTTAAAAAAAAA AAAGAAAGAAAGAAAGAAGGAAAGAAAGAGAGAGAGAGAAAAGAAAAAGAAGAAAGGAAGGAAGCAAGAA AAGGAGGAAGAAAGGAAAAGACAGAAGAGAAATGGAGGAGTCTGCTGCGCCTGGTTTTCAGCTTTGGTGA AGATGGATCCAGGCTTCATCTTTAATCACGCCAGGCCCGGGCCCATCTGTCTTGTTTACTCTGCCGAGGA GAAGACGGGCGAGCGAGCTTCGGTGGCGACCATTACCTCGACACTTGGCTAACAAATGAGGCCAGGCTCG GCCGCTGCCGCCGCCTCTGCTGCTGTCGCTGCTGGATCACAGCCTGGATTTTTCTTTCTTTGTCCCCTAC TCCTGACACCCAGCGAATGCACCCTCAGACTGCCAGATAGCACAGTGTTTTGGCCACGGTAACAAACACA CACACATATAACTTTCCTCCCTGTCTGTACCCACTTTGGGGTGGGGGTGGGTGGGAAGACTGCTCACTCC CTTCCACCATAGACTTAGAAGGGGGAACAGAAGGGAATTGAAGGGCAGTCTGCACAACGTGGGTTCCCAA ATCCGAGCCCAAGAAATAAATGAAAATGAAAAAAGAAAGGTAGCAATTGGGACACCCAAGAAGGCCTTCT GCTAGAAGGTCCAGCTAGGCCTGGCAGGGTGAGGGGCAGTTGAGTTCTGGGAGCTGGGAATGTCTTCTGG GCAGTTCACAGTAGTAGAGTCAAGGCCTTCTCTTAGGTTACAAATGAATGTGAAATTAGGAAATAAAATA CTGTGGCCCTCCTACTCTGGAAGGACAATGTTGCAGAACCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AATAAGGGAGCCGGGTAAAG; Reverse Primer - name:unspecified, sequence:GGGTTCTGCAACATTGTCCT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13807 same embryo
	EMAGE:13803 same embryo
	EMAGE:13804 same embryo
	EMAGE:13806 same embryo
	EurExpress:euxassay_019595 same experiment
	MGI:4825425 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source