EMAGE mouse gene expression spatial database

EMAGE:13811

Gpr133 G protein-coupled receptor 133 ( MGI:3041203)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019260. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019260_01	euxassay_019260_02	euxassay_019260_03	euxassay_019260_04

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euxassay_019260_20	euxassay_019260_21	euxassay_019260_22	euxassay_019260_23

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 09 10 18 19 20

thymus primordium

strong

regional

strong expression: see section 16 17 moderate expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35191
Entity Detected:	Gpr133, G protein-coupled receptor 133 ( MGI:3041203)
Sequence:	sense strand is shown >T35191 TCCCTTGTTATCGTATGCACAGAGCAACATCCCAGCAAGCCTGCATTAAATGACATGTAAAGATGGGGCT AGAGGACATAGCCTCAGCCTTGAAGGAGACTCACAGTCCTGAGGGCTACTCAATGTCTTATGGTTCTCCA GAGGGTCCACAGAGGTGGTAGCCGAGCCATGTAGGGGAATGAAGCCAGCACCAATTGGAATGTACATCTT CACGTAAGGGGACACTAATTGAGCCACCACCCACCCTAGCTGTACAGCCAGTTCTGGACTCTCCGCCTTT GCCCGTTTGGAGTCGCCCACTATCCCTTGTATGGATCTTTGTGAAGACAGTCTAAAGGAGCCACTTCATG GGACCCGATGCTGTCTATTCTCTGCAAGTTGTTCCTTGATGGACCTAAAGTCAGCCTTAGAATATTCTTT TCAAACACGAGCCTCTCTACTGTCCATGACCTCCTTGGGGGCTGAGCTTCCCTGCTGGGTGTCCCCCTGT ACCTGTATGTCCTGGCTTTCTGGGAGTGTTGCTACCCCTAGGGTCTGTGTTCTCATTATTTAGACGTGCC TCCTCCGTAAGAGCCAACAGGCATCAGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78968. Forward Primer - name:078968_F_cDNA_Gpr133, sequence:TCCCTTGTTATCGTATGCACAG; Reverse Primer - name:078968_N_SP6_cDNA_Gpr133, sequence:GTCTGATGCCTGTTGGCTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13813 same embryo
	EMAGE:13812 same embryo
	EurExpress:euxassay_019260 same experiment
	MGI:4825173 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source