EMAGE mouse gene expression spatial database

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EMAGE:13821

Ctcfl CCCTC-binding factor (zinc finger protein)-like ( MGI:3652571)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019660. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019660_01	euxassay_019660_02	euxassay_019660_03	euxassay_019660_04

euxassay_019660_05	euxassay_019660_06	euxassay_019660_07	euxassay_019660_08	euxassay_019660_09

euxassay_019660_10	euxassay_019660_11	euxassay_019660_12	euxassay_019660_13	euxassay_019660_14

euxassay_019660_15	euxassay_019660_16	euxassay_019660_17	euxassay_019660_18	euxassay_019660_19

euxassay_019660_20	euxassay_019660_21	euxassay_019660_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13821_wholemount_strong.wlz

13821_wholemount_moderate.wlz

13821_wholemount_weak.wlz

13821_wholemount_possible.wlz

13821_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13821_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

moderate

single cell

moderate expression: see section 01 02 03 19 weak expression: see section 04 05 06 07 08 20 22

pons mantle layer

moderate

single cell

moderate expression: see section 08 10 11 14 15 16 17

facial vii ganglion

moderate

single cell

moderate expression: see section 04 05 06

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 07 19 weak expression: see section 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 17 19 20 weak expression: see section 18

vagus x ganglion

moderate

single cell

moderate expression: see section 08 weak expression: see section 17

ventral grey horn

moderate

single cell

moderate expression: see section 13 14 16 weak expression: see section 10 11 15

dorsal root ganglion

moderate

single cell

moderate expression: see section 13 15 16 17 18 weak expression: see section 07 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55029
Entity Detected:	Ctcfl, CCCTC-binding factor (zinc finger protein)-like ( MGI:3652571)
Sequence:	sense strand is shown >T55029 GCTACCACGATGCTTTCAATGAATTCTAAAGTCAACCTGAGAATCTTAATGCTGCGGAAAGATGAATTGG TAGAAACAATGCTCATTTAACTAGAAAAAAAATATATATATATCATAGCTCTTTCTTTGGCATAGAGCAA GGCTCACCTTTAGTTTTTGCCCCTCCTCCCCCATCTTGTAGGCCTATGGGTTAAACCCAGGGCTTGGCAC AATTTAGACAAGTGCCCCACCACCAGGCCATACCCCCAGCCCCCACACAACTTCCAAAAAAAATTAAATT ATCCAGGCTGGATTTGAACTCAATTTGGAGTCCAGCTGCACTTCAAACTGTCCATTCTCCTGCCTCCGTC TTGCAGGAATGACGAGCCTTTACCACCAGGCCGGGCTTGTCTTCTCTTAATTGTAGTCTTCGCCCGCTAT GAAATTACACTGGCATTGTTGCAAGCATTGGCTTTGTTC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCTACCACGATGCTTTCAATG; Reverse Primer - name:unspecified, sequence:GAACAAAGCCAATGCTTGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13822 same embryo
	EMAGE:13823 same embryo
	EMAGE:13820 same embryo
	EurExpress:euxassay_019660 same experiment
	MGI:4824099 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source