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EMAGE:13826

Pou1f1 POU domain, class 1, transcription factor 1 ( MGI:97588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826
"Pseudo-wholemount" of euxassay_019621. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019621_01 euxassay_019621_02 euxassay_019621_03 euxassay_019621_04
EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826
euxassay_019621_05 euxassay_019621_06 euxassay_019621_07 euxassay_019621_08 euxassay_019621_09
EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826
euxassay_019621_10 euxassay_019621_11 euxassay_019621_12 euxassay_019621_13 euxassay_019621_14
EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826 EMAGE:13826
euxassay_019621_15 euxassay_019621_16 euxassay_019621_17 euxassay_019621_18 euxassay_019621_19
EMAGE:13826 EMAGE:13826 EMAGE:13826
euxassay_019621_20 euxassay_019621_21 euxassay_019621_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13826Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13826_wholemount_strong.wlz
13826_wholemount_moderate.wlz
13826_wholemount_weak.wlz
13826_wholemount_possible.wlz
13826_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13826_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pituitary gland
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 weak expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55153
Entity Detected:Pou1f1, POU domain, class 1, transcription factor 1 ( MGI:97588)
Sequence:sense strand is shown

>T55153
TTTCACCTCGGCTGATACCTTTATAACTCTGAATTCTGACGCTTCTGCTGCCCTGCCTCTGAGAATGCAC
CACAGTGCCGCTGAGTGTCTCCCAGCCTCCAACCACGCCACCAACGTGATGTCCACAGCGACAGGACTTC
ATTACTCTGTGCCTTCCTGTCATTATGGAAACCAGCCATCCACCTATGGAGTGATGGCAGGCAGTTTAAC
CCCTTGTCTTTACAAGTTTCCAGACCACACCCTGAGTCATGGATTTCCCCCCCTGCACCAGCCTCTGCTG
GCAGAGGACCCCGCGGCCTCTGAGTTTAAGCAGGAACTCAGGCGGAAAAGCAAATTGGTGGAAGAGCCAA
TAGACATGGACTCCCCAGAAATCCGAGAACTGGAGCAGTTTGCCAACGAGTTTAAAGTGAGAAGAATTAA
GTTAGGATACACCCAGACAAACGTGGGCGAAGCGCTGGCTGCTGTCCACGGCTCAGAATTCAGTCAAACA
ACCATCTGTCGATTTGAAAACTTGCAGCTCAGTTTCAAAAATGCTTGTAAACTCAAAGCAATTTTATCCA
AGTGGCTGGAGGAAGCTGAGCAGGTCGGAGCTTTGTACAATGAGAAGGTGGGAGCAAACGAAAGGAAGAG
GAAACGGAGGACAACCATCAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTTCACCTCGGCTGATACCT; Reverse Primer - name:unspecified, sequence:CTGATGGTTGTCCTCCGTTT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13828 same embryo
 EMAGE:13827 same embryo
 EMAGE:13824 same embryo
 EMAGE:13825 same embryo
 EurExpress:euxassay_019621 same experiment
 MGI:4827338 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS